ID   GTFC_AMYOR              Reviewed;         409 AA.
AC   P96560;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   28-JAN-2026, entry version 73.
DE   RecName: Full=Glycosyltransferase GtfC;
DE            EC=2.4.1.-;
GN   Name=gtfC;
OS   Amycolatopsis orientalis (Nocardia orientalis).
OC   Bacteria; Bacillati; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC   Pseudonocardiaceae; Amycolatopsis.
OX   NCBI_TaxID=31958;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9115410; DOI=10.1016/s1074-5521(97)90288-x;
RA   Solenberg P.J., Matsushima P., Stack D.R., Wilkie S.C., Thompson R.C.,
RA   Baltz R.H.;
RT   "Production of hybrid glycopeptide antibiotics in vitro and in Streptomyces
RT   toyocaensis.";
RL   Chem. Biol. 4:195-202(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PATHWAY.
RX   PubMed=9545426; DOI=10.1016/s1074-5521(98)90060-6;
RA   van Wageningen A., Kirkpatrick P., Williams D., Harris B., Kershaw J.,
RA   Lennard N., Jones M., Jones S., Solenberg P.;
RT   "Sequencing and analysis of genes involved in the biosynthesis of a
RT   vancomycin group antibiotic.";
RL   Chem. Biol. 5:155-162(1998).
RN   [3]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=11294642; DOI=10.1021/bi010050w;
RA   Losey H.C., Peczuh M.W., Chen Z., Eggert U.S., Dong S.D., Pelczer I.,
RA   Kahne D., Walsh C.T.;
RT   "Tandem action of glycosyltransferases in the maturation of vancomycin and
RT   teicoplanin aglycones: novel glycopeptides.";
RL   Biochemistry 40:4745-4755(2001).
RN   [4]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=15070728; DOI=10.1073/pnas.0400277101;
RA   Lu W., Oberthuer M., Leimkuhler C., Tao J., Kahne D., Walsh C.T.;
RT   "Characterization of a regiospecific epivancosaminyl transferase GtfA and
RT   enzymatic reconstitution of the antibiotic chloroeremomycin.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:4390-4395(2004).
CC   -!- FUNCTION: Catalyzes the attachment of dTDP-L-4-epi-vancosamine to
CC       chloroorienticin B to form chloroeremomycin in the biosynthesis of
CC       glycopeptide antibiotic chloroeremomycin, a member of the vancomycin
CC       group of antibiotics. Also able to use dTDP-L-4-epi-vancosamine and
CC       devancoaminyl-vancomycin (DVV) to create epivancomycin. Acts downstream
CC       of GtfA. {ECO:0000269|PubMed:11294642, ECO:0000269|PubMed:15070728}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=dTDP-beta-L-vancosamine + devancoaminyl-vancomycin =
CC         epivancomycin + dTDP + H(+); Xref=Rhea:RHEA:45904, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58369, ChEBI:CHEBI:75953, ChEBI:CHEBI:76839,
CC         ChEBI:CHEBI:85491; Evidence={ECO:0000269|PubMed:11294642,
CC         ECO:0000269|PubMed:15070728};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=chloroorienticin B + dTDP-beta-L-vancosamine =
CC         chloroeremomycin + dTDP + H(+); Xref=Rhea:RHEA:45908,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:58369, ChEBI:CHEBI:75963,
CC         ChEBI:CHEBI:76839, ChEBI:CHEBI:85488;
CC         Evidence={ECO:0000269|PubMed:11294642, ECO:0000269|PubMed:15070728};
CC   -!- PATHWAY: Antibiotic biosynthesis; vancomycin biosynthesis.
CC       {ECO:0000269|PubMed:9545426}.
CC   -!- MISCELLANEOUS: In A.orientalis different glycosyltransferases are
CC       involved in biosynthesis of the vancomycin group of antibiotics. GtfA
CC       (AC P96558), GtfB (AC P96559) and GtfC are involved in biosynthesis of
CC       antibiotic chloroeremomycin, while GtfD (AC Q9AFC7) and GtfE (AC
CC       G4V4R9) are involved in biosynthesis of vancomycin.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
CC       {ECO:0000305}.
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DR   EMBL; U84349; AAB49294.1; -; Genomic_DNA.
DR   EMBL; AJ223998; CAA11776.1; -; Genomic_DNA.
DR   PIR; T30586; T30586.
DR   AlphaFoldDB; P96560; -.
DR   SMR; P96560; -.
DR   CAZy; GT1; Glycosyltransferase Family 1.
DR   KEGG; ag:CAA11776; -.
DR   UniPathway; UPA00162; -.
DR   GO; GO:0016758; F:hexosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0008194; F:UDP-glycosyltransferase activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0033072; P:vancomycin biosynthetic process; IDA:UniProtKB.
DR   CDD; cd03784; GT1_Gtf-like; 1.
DR   FunFam; 3.40.50.2000:FF:000292; Glycosyltransferase GtfE; 1.
DR   FunFam; 3.40.50.2000:FF:000009; Sterol 3-beta-glucosyltransferase UGT80A2; 1.
DR   Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR   InterPro; IPR010610; EryCIII-like_C.
DR   InterPro; IPR050426; Glycosyltransferase_28.
DR   InterPro; IPR004276; GlycoTrans_28_N.
DR   InterPro; IPR002213; UDP_glucos_trans.
DR   PANTHER; PTHR48050; STEROL 3-BETA-GLUCOSYLTRANSFERASE; 1.
DR   PANTHER; PTHR48050:SF13; STEROL 3-BETA-GLUCOSYLTRANSFERASE UGT80A2; 1.
DR   Pfam; PF06722; EryCIII-like_C; 1.
DR   Pfam; PF03033; Glyco_transf_28; 1.
DR   SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; Glycosyltransferase; Transferase.
FT   CHAIN           1..409
FT                   /note="Glycosyltransferase GtfC"
FT                   /id="PRO_0000430438"
SQ   SEQUENCE   409 AA;  42920 MW;  3244092F8E26F82B CRC64;
     MRVLLSTAGS RGDVEPLVAL AVRLQGLGVE ARMCASPASA ERLAEVGVPH VPVGLQLEGM
     LLQEGMPPPS PEEERRLAAK AIDMQFDEVP AAAEGCAAVV AAGELAAAAA VRSVAEMLGI
     PYFYAAYSPN YLPSPHHAPP EDERTTPGVT DNKVLWDERG QRFAKRYGDT LNSRRASVGL
     PPVEDVFGYG YSERPWLATD PILAPLPPDF DAVQTGTWIL PDERPLSAEL EAFLAAGSPP
     VYLGFGSASG PGIDDAARVA IEAIRAHGRR IVLLSGWADL VRPDDGADCF SVDEVNLQVL
     FSRAAAAIHH GSAGTEHLAT LAGIPQIVIP RHTDQPYYAE RVADLGIGVA LEGPVPTFDA
     MSAAVATALA PETRARATAV AGTIRTDGAA VAARLLLDAV SREKSAVLA
//