ID   LCFA_BACSU              Reviewed;         560 AA.
AC   P94547;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   04-NOV-2026, entry version 134.
DE   RecName: Full=Long-chain-fatty-acid--CoA ligase;
DE            EC=6.2.1.3;
DE   AltName: Full=Long-chain acyl-CoA synthetase;
GN   Name=lcfA; OrderedLocusNames=BSU28560;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Bacillati; Bacillota; Bacilli; Caryophanales; Bacillaceae;
OC   Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969504; DOI=10.1099/13500872-142-11-3067;
RA   Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J.,
RA   Emmerson P.T., Harwood C.R.;
RT   "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus subtilis
RT   chromosome containing genes responsible for stress responses, the
RT   utilization of plant cell walls and primary metabolism.";
RL   Microbiology 142:3067-3078(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a long-chain fatty acid + ATP + CoA = a long-chain fatty acyl-
CC         CoA + AMP + diphosphate; Xref=Rhea:RHEA:15421, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57287, ChEBI:CHEBI:57560,
CC         ChEBI:CHEBI:83139, ChEBI:CHEBI:456215; EC=6.2.1.3;
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; Z75208; CAA99571.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14816.1; -; Genomic_DNA.
DR   PIR; D69649; D69649.
DR   RefSeq; NP_390734.1; NC_000964.3.
DR   RefSeq; WP_004399166.1; NC_000964.3.
DR   AlphaFoldDB; P94547; -.
DR   SMR; P94547; -.
DR   FunCoup; P94547; 457.
DR   STRING; 224308.BSU28560; -.
DR   PaxDb; 224308-BSU28560; -.
DR   EnsemblBacteria; CAB14816; CAB14816; BSU_28560.
DR   GeneID; 936505; -.
DR   KEGG; bsu:BSU28560; -.
DR   PATRIC; fig|224308.179.peg.3103; -.
DR   eggNOG; COG0318; Bacteria.
DR   InParanoid; P94547; -.
DR   OMA; AAAHCHA; -.
DR   OrthoDB; 9803968at2; -.
DR   PhylomeDB; P94547; -.
DR   BioCyc; BSUB:BSU28560-MONOMER; -.
DR   Proteomes; UP000001570; Chromosome.
DR   AbasyAtlas; P94547; -.
DR   GO; GO:0016405; F:CoA-ligase activity; IBA:GO_Central.
DR   GO; GO:0004467; F:long-chain fatty acid-CoA ligase activity; IEA:UniProtKB-EC.
DR   CDD; cd05936; FC-FACS_FadD_like; 1.
DR   FunFam; 3.30.300.30:FF:000008; 2,3-dihydroxybenzoate-AMP ligase; 1.
DR   Gene3D; 3.30.300.30; -; 1.
DR   Gene3D; 3.40.50.980; -; 2.
DR   Gene3D; 2.30.38.10; Luciferase, Domain 3; 1.
DR   InterPro; IPR045851; AMP-b_sf.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_synth/lig_dom.
DR   InterPro; IPR050237; ATP-dep_AMP-bd_enzyme.
DR   PANTHER; PTHR43767; LONG-CHAIN-FATTY-ACID--COA LIGASE; 1.
DR   PANTHER; PTHR43767:SF9; LONG-CHAIN-FATTY-ACID--COA LIGASE; 1.
DR   Pfam; PF00501; AMP-binding; 1.
DR   Pfam; PF13193; AMP-binding_C; 1.
DR   SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Fatty acid metabolism; Ligase; Lipid metabolism;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..560
FT                   /note="Long-chain-fatty-acid--CoA ligase"
FT                   /id="PRO_0000193124"
SQ   SEQUENCE   560 AA;  62692 MW;  416A664E5926C7EA CRC64;
     MQSQKPWLAE YPNDIPHELP LPNKTLQSIL TDSAARFPDK TAISFYGKKL TFHDILTDAL
     KLAAFLQCNG LQKGDRVAVM LPNCPQTVIS YYGVLFAGGI VVQTNPLYTE HELEYQLRDA
     QVSVIITLDL LFPKAIKMKT LSIVDQILIT SVKDYLPFPK NILYPLTQKQ KVHIDFDKTA
     NIHTFASCMK QEKTELLTIP KIDPEHDIAV LQYTGGTTGA PKGVMLTHQN ILANTEMCAA
     WMYDVKEGAE KVLGIVPFFH VYGLTAVMNY SIKLGFEMIL LPKFDPLETL KIIDKHKPTL
     FPGAPTIYIG LLHHPELQHY DLSSIKSCLS GSAALPVEVK QKFEKVTGGK LVEGYGLSEA
     SPVTHANFIW GKNKPGSIGC PWPSTDAAIY SEETGELAAP YEHGEIIVKG PQVMKGYWNK
     PEETAAVLRD GWLFTGDMGY MDEEGFFYIA DRKKDIIIAG GYNIYPREVE EALYEHEAIQ
     EIVVAGVPDS YRGETVKAFV VLKKGAKADT EELDAFARSR LAPYKVPKAY EFRKELPKTA
     VGKILRRRLL EEETENHHIK
//