ID   CP51_WHEAT              Reviewed;         453 AA.
AC   P93596; P93595;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1997, sequence version 1.
DT   02-SEP-2026, entry version 136.
DE   RecName: Full=Obtusifoliol 14-alpha demethylase;
DE            EC=1.14.14.154;
DE   AltName: Full=CYPLI;
DE   AltName: Full=Cytochrome P450 51;
DE   AltName: Full=Cytochrome P450-LIA1;
DE   Flags: Fragment;
GN   Name=CYP51;
OS   Triticum aestivum (Wheat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX   NCBI_TaxID=4565;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Darius; TISSUE=Shoot;
RX   PubMed=9016788; DOI=10.1006/bbrc.1996.5873;
RA   Cabello-Hurtado F., Zimmerlin A., Rahier A., Taton M., Derose R.,
RA   Nedelkina S., Batard Y., Durst F., Pallett K.E., Werck-Reichhart D.;
RT   "Cloning and functional expression in yeast of a cDNA coding for an
RT   obtusifoliol 14alpha-demethylase (CYP51) in wheat.";
RL   Biochem. Biophys. Res. Commun. 230:381-385(1997).
CC   -!- FUNCTION: Catalyzes the 14-alpha demethylation of obtusifoliol to 4
CC       alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 14alpha-methyl steroid + 3 reduced [NADPH--hemoprotein
CC         reductase] + 3 O2 = a Delta(14) steroid + formate + 3 oxidized
CC         [NADPH--hemoprotein reductase] + 4 H2O + 4 H(+);
CC         Xref=Rhea:RHEA:54028, Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:138029, ChEBI:CHEBI:138031; EC=1.14.14.154;
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413;
CC   -!- PATHWAY: Steroid biosynthesis; zymosterol biosynthesis; zymosterol from
CC       lanosterol: step 1/6.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}. Membrane; Single-pass
CC       membrane protein.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR   EMBL; Y09291; CAA70475.1; -; mRNA.
DR   EMBL; Y09292; CAA70476.1; -; mRNA.
DR   PIR; T06475; T06475.
DR   AlphaFoldDB; P93596; -.
DR   SMR; P93596; -.
DR   STRING; 4565.P93596; -.
DR   PaxDb; 4565-P93596; -.
DR   eggNOG; KOG0684; Eukaryota.
DR   UniPathway; UPA00770; UER00754.
DR   Proteomes; UP000019116; Unplaced.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0008398; F:sterol 14-demethylase activity; IEA:UniProtKB-EC.
DR   CDD; cd11042; CYP51-like; 1.
DR   FunFam; 1.10.630.10:FF:000028; Cytochrome p450 51g1; 1.
DR   Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR   InterPro; IPR050529; CYP51A1-like.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   PANTHER; PTHR24304:SF2; 24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE; 1.
DR   PANTHER; PTHR24304; CYTOCHROME P450 FAMILY 7; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00465; EP450IV.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; Cytochrome P450; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Heme; Iron; Lipid biosynthesis; Lipid metabolism; Membrane; Metal-binding;
KW   Methyltransferase; Monooxygenase; Oxidoreductase; Reference proteome;
KW   Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis;
KW   Sterol metabolism; Transferase.
FT   CHAIN           <1..453
FT                   /note="Obtusifoliol 14-alpha demethylase"
FT                   /id="PRO_0000052014"
FT   BINDING         395
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250"
FT   VARIANT         150
FT                   /note="Missing (in clone W516)"
FT   VARIANT         175
FT                   /note="L -> Q (in clone W516)"
FT   VARIANT         443
FT                   /note="N -> S (in clone W516)"
FT   VARIANT         450
FT                   /note="I -> V (in clone W516)"
FT   NON_TER         1
SQ   SEQUENCE   453 AA;  51644 MW;  12A3D00952E3907D CRC64;
     RPPPTIPGAP VVGGLLRFLR GPIPLIRAEY ARLGPVFTVP ILTRRITFLI GPDVSAHFFK
     SNESDMSQQE VYRFNVPTFG PGVVFDVDYQ VRQEQFRFFT EALRANKLRS YVDQMVAEAE
     EYFSKWGESG TVDLKYELEH LIILTASRCL LGREVREKLF DDVSALFHDL DNGMLPISVI
     FPYLPIPAHR RRDQARTRLA EIFATIIKSR KASGQSEEDM LQCFIDSKYK NGRQTTESEV
     TGLLIAALFA GQHTSSITST WTGAYLLKFQ QYFAEAVEEQ KEVMKRHGDK IDHDILAEMD
     VLYRCIKEAL RLHPPLIMLL RQSHSDFSVT TREGKEFDIP KGHIVATSPA FANRLPHIFK
     NPDSYDPDRF AAGREEDKVA GAFSYISFGG GRHGCLGEPF AYLQIKAIWT HLLRNFEFEL
     VSPFPENDWN AMVVGIKGEV MVNYKRRKLI VDN
//