ID XTH_TOBAC Reviewed; 295 AA. AC P93349; DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot. DT 01-MAY-1997, sequence version 1. DT 04-NOV-2026, entry version 109. DE RecName: Full=Probable xyloglucan endotransglucosylase/hydrolase protein; DE EC=2.4.1.207; DE Flags: Precursor; GN Name=XTH; Synonyms=EXGT; OS Nicotiana tabacum (Common tobacco). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; OC Nicotiana. OX NCBI_TaxID=4097; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RA Nishitani K., Okazawa K., Takeda S., Asada K., Kato I.; RT "Endo-xyloglucan transferase (EXGT)."; RL Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases. RN [2] RP SUBCELLULAR LOCATION. RX PubMed=11266580; DOI=10.1093/pcp/pce034; RA Yokoyama R., Nishitani K.; RT "Endoxyloglucan transferase is localized both in the cell plate and in the RT secretory pathway destined for the apoplast in tobacco cells."; RL Plant Cell Physiol. 42:292-300(2001). CC -!- FUNCTION: Catalyzes xyloglucan endohydrolysis (XEH) and/or CC endotransglycosylation (XET). Cleaves and religates xyloglucan CC polymers, an essential constituent of the primary cell wall, and CC thereby participates in cell wall construction of growing tissues. CC -!- CATALYTIC ACTIVITY: CC Reaction=breaks a beta-(1->4) bond in the backbone of a xyloglucan and CC transfers the xyloglucanyl segment on to O-4 of the non-reducing CC terminal glucose residue of an acceptor, which can be a xyloglucan or CC an oligosaccharide of xyloglucan.; EC=2.4.1.207; CC -!- SUBCELLULAR LOCATION: Secreted, cell wall CC {ECO:0000269|PubMed:11266580}. Secreted, extracellular space, apoplast CC {ECO:0000269|PubMed:11266580}. CC -!- PTM: Contains at least one intrachain disulfide bond essential for its CC enzymatic activity. {ECO:0000250}. CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 16 family. XTH group 1 CC subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D86730; BAA13163.1; -; mRNA. DR RefSeq; NP_001312331.1; NM_001325402.1. DR AlphaFoldDB; P93349; -. DR SMR; P93349; -. DR STRING; 4097.P93349; -. DR CAZy; GH16; Glycoside Hydrolase Family 16. DR GlyCosmos; P93349; 1 site, No reported glycans. DR PaxDb; 4097-P93349; -. DR GeneID; 107785465; -. DR KEGG; nta:107785465; -. DR OMA; ADHIRYI; -. DR OrthoDB; 4781at2759; -. DR PhylomeDB; P93349; -. DR BRENDA; 2.4.1.207; 3645. DR Proteomes; UP000790787; Chromosome 7. DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell. DR GO; GO:0009505; C:plant-type cell wall; IBA:GO_Central. DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro. DR GO; GO:0030247; F:polysaccharide binding; ISS:UniProtKB. DR GO; GO:0016762; F:xyloglucan:xyloglucosyl transferase activity; IBA:GO_Central. DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-ARBA. DR GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IBA:GO_Central. DR GO; GO:0010411; P:xyloglucan metabolic process; IBA:GO_Central. DR CDD; cd02176; GH16_XET; 1. DR FunFam; 2.60.120.200:FF:000025; Xyloglucan endotransglucosylase/hydrolase; 1. DR Gene3D; 2.60.120.200; -; 1. DR InterPro; IPR000757; Beta-glucanase-like. DR InterPro; IPR044791; Beta-glucanase/XTH. DR InterPro; IPR013320; ConA-like_dom_sf. DR InterPro; IPR008263; GH16_AS. DR InterPro; IPR010713; XET_C. DR InterPro; IPR016455; XTH. DR PANTHER; PTHR31062; XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED; 1. DR Pfam; PF00722; Glyco_hydro_16; 1. DR Pfam; PF06955; XET_C; 1. DR PIRSF; PIRSF005604; XET; 1. DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1. DR PROSITE; PS01034; GH16_1; 1. DR PROSITE; PS51762; GH16_2; 1. PE 2: Evidence at transcript level; KW Apoplast; Cell wall; Cell wall biogenesis/degradation; Disulfide bond; KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal; KW Transferase. FT SIGNAL 1..21 FT /evidence="ECO:0000255" FT CHAIN 22..295 FT /note="Probable xyloglucan endotransglucosylase/hydrolase FT protein" FT /id="PRO_0000011840" FT DOMAIN 22..220 FT /note="GH16" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01098" FT ACT_SITE 106 FT /note="Nucleophile" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10064" FT ACT_SITE 110 FT /note="Proton donor" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10064" FT BINDING 110 FT /ligand="xyloglucan" FT /ligand_id="ChEBI:CHEBI:18233" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT BINDING 123..125 FT /ligand="xyloglucan" FT /ligand_id="ChEBI:CHEBI:18233" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT BINDING 133..135 FT /ligand="xyloglucan" FT /ligand_id="ChEBI:CHEBI:18233" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT BINDING 199..200 FT /ligand="xyloglucan" FT /ligand_id="ChEBI:CHEBI:18233" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT BINDING 204 FT /ligand="xyloglucan" FT /ligand_id="ChEBI:CHEBI:18233" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT BINDING 281 FT /ligand="xyloglucan" FT /ligand_id="ChEBI:CHEBI:18233" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT SITE 108 FT /note="Important for catalytic activity" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT CARBOHYD 114 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT DISULFID 228..239 FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT DISULFID 276..289 FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" SQ SEQUENCE 295 AA; 33882 MW; D379980DC2DA1765 CRC64; MGVKGLLFSI VLINLSLLGL CGYPRKPVDV PFWKNYEPSW ASHHIKYLSG GSTVDLVLDR SSGAGFQSKK SYLFGHFSMK LKLVGGDSAG VVTAFYLSSN NAEHDEIDFE FLGNRTGQPY ILQTNVFTGG KGDREQRIYL WFDPTKGYHS YSVLWNTFQI VIFVDDVPIR AFKNSKDLGV KFPFNQPMKI YSSLWDADDW ATRGGLEKTD WSNAPFTASY TSFHVDGCEA ATPQEVQVCN TKGMRWWDQK AFQDLDALQY RRLRWVRQKY TIYNYCTDRK RYPTLPPECT KDRDI //