ID P93067_BRAOL Unreviewed; 1025 AA. AC P93067; DT 01-MAY-1997, integrated into UniProtKB/TrEMBL. DT 01-MAY-1997, sequence version 1. DT 04-NOV-2026, entry version 146. DE RecName: Full=Calcium-transporting ATPase {ECO:0000256|RuleBase:RU361146}; DE EC=7.2.2.10 {ECO:0000256|RuleBase:RU361146}; OS Brassica oleracea (Wild cabbage). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica. OX NCBI_TaxID=3712 {ECO:0000313|EMBL:CAA68234.1}; RN [1] {ECO:0000313|EMBL:CAA68234.1} RP NUCLEOTIDE SEQUENCE. RC TISSUE=Inflorescence {ECO:0000313|EMBL:CAA68234.1}; RX PubMed=9009223; DOI=10.1016/S0014-5793(96)01448-2; RA Malmstrom S., Askerlund P., Palmgren M.G.; RT "A calmodulin-stimulated Ca2+-ATPase from plant vacuolar membranes with a RT putative regulatory domain at its N-terminus."; RL FEBS Lett. 400:324-328(1997). CC -!- FUNCTION: Catalyzes the hydrolysis of ATP coupled with the transport of CC calcium. {ECO:0000256|RuleBase:RU361146}. CC -!- CATALYTIC ACTIVITY: CC Reaction=Ca(2+)(in) + ATP + H2O = Ca(2+)(out) + ADP + phosphate + H(+); CC Xref=Rhea:RHEA:18105, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, CC ChEBI:CHEBI:29108, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, CC ChEBI:CHEBI:456216; EC=7.2.2.10; CC Evidence={ECO:0000256|ARBA:ARBA00048694, CC ECO:0000256|RuleBase:RU361146}; CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141, CC ECO:0000256|RuleBase:RU361146}; Multi-pass membrane protein CC {ECO:0000256|ARBA:ARBA00004141, ECO:0000256|RuleBase:RU361146}. CC -!- SIMILARITY: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) CC family. Type IIB subfamily. {ECO:0000256|ARBA:ARBA00006124, CC ECO:0000256|RuleBase:RU361146}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; X99972; CAA68234.1; -; mRNA. DR PIR; T14453; T14453. DR AlphaFoldDB; P93067; -. DR BioCyc; MetaCyc:MONOMER-14662; -. DR GO; GO:0005886; C:plasma membrane; IEA:TreeGrafter. DR GO; GO:0005524; F:ATP binding; IEA:InterPro. DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro. DR GO; GO:0005516; F:calmodulin binding; IEA:InterPro. DR GO; GO:0005388; F:P-type calcium transporter activity; IEA:UniProtKB-EC. DR CDD; cd02081; P-type_ATPase_Ca_PMCA-like; 1. DR FunFam; 1.20.1110.10:FF:000039; Calcium-transporting ATPase; 1. DR FunFam; 1.20.5.170:FF:000026; Calcium-transporting ATPase; 1. DR FunFam; 2.70.150.10:FF:000006; Calcium-transporting ATPase; 1. DR FunFam; 3.40.1110.10:FF:000011; Calcium-transporting ATPase; 1. DR FunFam; 3.40.50.1000:FF:000144; copper-transporting ATPase 1 isoform X2; 1. DR Gene3D; 1.20.5.170; -; 1. DR Gene3D; 3.40.1110.10; Calcium-transporting ATPase, cytoplasmic domain N; 1. DR Gene3D; 2.70.150.10; Calcium-transporting ATPase, cytoplasmic transduction domain A; 1. DR Gene3D; 1.20.1110.10; Calcium-transporting ATPase, transmembrane domain; 1. DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 1. DR InterPro; IPR006068; ATPase_P-typ_cation-transptr_C. DR InterPro; IPR004014; ATPase_P-typ_cation-transptr_N. DR InterPro; IPR023299; ATPase_P-typ_cyto_dom_N. DR InterPro; IPR018303; ATPase_P-typ_P_site. DR InterPro; IPR023298; ATPase_P-typ_TM_dom_sf. DR InterPro; IPR008250; ATPase_P-typ_transduc_dom_A_sf. DR InterPro; IPR059000; ATPase_P-type_domA. DR InterPro; IPR024750; Ca_ATPase_N_dom. DR InterPro; IPR036412; HAD-like_sf. DR InterPro; IPR023214; HAD_sf. DR InterPro; IPR006408; P-type_ATPase_IIB. DR InterPro; IPR001757; P_typ_ATPase. DR InterPro; IPR044492; P_typ_ATPase_HD_dom. DR NCBIfam; TIGR01517; ATPase-IIB_Ca; 1. DR NCBIfam; TIGR01494; ATPase_P-type; 3. DR PANTHER; PTHR24093:SF462; CALCIUM-TRANSPORTING ATPASE 11, PLASMA MEMBRANE-TYPE-RELATED; 1. DR PANTHER; PTHR24093; CATION TRANSPORTING ATPASE; 1. DR Pfam; PF12515; CaATP_NAI; 1. DR Pfam; PF13246; Cation_ATPase; 1. DR Pfam; PF00689; Cation_ATPase_C; 1. DR Pfam; PF00690; Cation_ATPase_N; 1. DR Pfam; PF00122; E1-E2_ATPase; 1. DR PRINTS; PR00119; CATATPASE. DR PRINTS; PR00120; HATPASE. DR SFLD; SFLDG00002; C1.7:_P-type_atpase_like; 1. DR SFLD; SFLDS00003; Haloacid_Dehalogenase; 1. DR SFLD; SFLDF00027; p-type_atpase; 1. DR SMART; SM00831; Cation_ATPase_N; 1. DR SUPFAM; SSF81653; Calcium ATPase, transduction domain A; 1. DR SUPFAM; SSF81665; Calcium ATPase, transmembrane domain M; 1. DR SUPFAM; SSF56784; HAD-like; 1. DR SUPFAM; SSF81660; Metal cation-transporting ATPase, ATP-binding domain N; 1. DR PROSITE; PS00154; ATPASE_E1_E2; 1. PE 2: Evidence at transcript level; KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU361146}; KW Calcium {ECO:0000256|ARBA:ARBA00022837, ECO:0000256|RuleBase:RU361146}; KW Calcium transport {ECO:0000256|ARBA:ARBA00022568, KW ECO:0000256|RuleBase:RU361146}; KW Calmodulin-binding {ECO:0000256|ARBA:ARBA00022860}; KW Ion transport {ECO:0000256|ARBA:ARBA00023065, KW ECO:0000256|RuleBase:RU361146}; Magnesium {ECO:0000256|ARBA:ARBA00022842}; KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU361146}; KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}; KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, KW ECO:0000256|RuleBase:RU361146}; KW Translocase {ECO:0000256|ARBA:ARBA00022967}; KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, KW ECO:0000256|RuleBase:RU361146}; KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, KW ECO:0000256|RuleBase:RU361146}; KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU361146}. FT TRANSMEM 169..188 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361146" FT TRANSMEM 194..214 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361146" FT TRANSMEM 351..373 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361146" FT TRANSMEM 393..422 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361146" FT TRANSMEM 809..829 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361146" FT TRANSMEM 835..853 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361146" FT TRANSMEM 881..899 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361146" FT TRANSMEM 911..929 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361146" FT TRANSMEM 941..965 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361146" FT TRANSMEM 980..1000 FT /note="Helical" FT /evidence="ECO:0000256|RuleBase:RU361146" FT DOMAIN 111..186 FT /note="Cation-transporting P-type ATPase N-terminal" FT /evidence="ECO:0000259|SMART:SM00831" SQ SEQUENCE 1025 AA; 111770 MW; 9D6585FD5BED783F CRC64; MSNLLKDFQV EAKNPSLEAR QRWRSSVSIV KNRARRFRMI SNLEKLAENE KKRCQIQEKI RVAFYVQKAA LQFIDAGTRR EYKLTDEVRQ AGFHVEADEL ASMVRNHDTR SLTKSGGAEG IAQKLSVSLT EGVRSNDLDI REKIYGANRY AEKPARSFLT FVWEALQDVT LIILMVCAVV SIGVGVATEG FPKGMYDGTG ILLSIILVVM VTAISDYRQS LQFRDLDREK KKINIQVTRD GNRQEVSIDD LVVGDVVHLS IGDRVPADGV FISGYNLEID ESSLSGESEP SHVNKEKPFL LSGTKVQNGS AKMLVTTVGM RTEWGKLMET LSEGGEDETP LQVKLNGVAT IIGKIGLGFA VLTFVVLCVR FVIGKAAAGG ISEWSSEDAL TLLDYFAIAV TIIVVAVPEG LPLAVTLSLA FAMKQLMKDR ALVRHLAACE TMGSSTCICT DKTGTLTTNH MVVNKVWICE NIKERREENF ELNLSEQVKN ILIQAIFQNT GSEVVKDKEG KTQILGSPTE RAILEFGLLL GGDVEMQGRE HKILKIEPFN SDKKKMSVLT SHSGGKVRAF CKGASEIVLR MCEKVVDSSG KSVPLSEEKI AAVSEVIEGF ASEALRTLCL VYTDLDEAPS GDLPDGGYTL VAVVGIKDPV RPGVRKAVQT CQNAGITVRM VTGDNISTAK AIAKECGILT AGGVAIEGSE FRNLPPHEMR AILPKIQVMA RSLPLDKHTL VNNLRKIGEV VAVTGDGTND APALHESDIG LAMGIAGTEV AKENADVIIM DDNFATIVNV ARWGRAVYIN IQKFVQFQLT VNVVALIINF VSACITGSAP LTAVHLLWVN MIMDTLGALA LATEPPNEGL MKRQPIGRTA SFITRAMWRN IIGQSIYQLI VLGILNFYGK QILDLNGPDS TAVLNTIIFN SFVFCQVFNE VNSREIEKIN VFAGMFSSWV FVAVMTATTG FQLIIVELLG AFASTVPLSW QHWLLCIVIG SISMILAVGL KCIPVESNSH HDGYELLPSG PSDSA //