ID   CDC5L_ARATH             Reviewed;         844 AA.
AC   P92948; B9DFT0; O04498; Q6R0C5;
DT   09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT   09-FEB-2010, sequence version 2.
DT   02-SEP-2026, entry version 180.
DE   RecName: Full=Cell division cycle 5-like protein;
DE            Short=Cdc5-like protein;
DE   AltName: Full=Atypical R2R3-MYB transcription factor CDC5;
DE   AltName: Full=MOS4-associated complex protein 1;
DE            Short=MAC protein 1;
DE   AltName: Full=Protein MYB DOMAIN CELL DIVISION CYCLE 5;
DE            Short=AtMYBCD5;
GN   Name=CDC5; Synonyms=MAC1, MYBCD5; OrderedLocusNames=At1g09770;
GN   ORFNames=F21M12.15;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC   STRAIN=cv. Columbia;
RX   PubMed=8917598; DOI=10.1073/pnas.93.23.13371;
RA   Hirayama T., Shinozaki K.;
RT   "A cdc5+ homolog of a higher plant, Arabidopsis thaliana.";
RL   Proc. Natl. Acad. Sci. U.S.A. 93:13371-13376(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND GENE FAMILY.
RC   STRAIN=cv. Columbia;
RX   PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA   Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA   Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA   Qu L.-J.;
RT   "The MYB transcription factor superfamily of Arabidopsis: expression
RT   analysis and phylogenetic comparison with the rice MYB family.";
RL   Plant Mol. Biol. 60:107-124(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RG   The Arabidopsis Information Resource (TAIR);
RT   "Community-consensus reference genome of Arabidopsis thaliana Col-0.";
RL   Submitted (FEB-2026) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-504.
RC   STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX   PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA   Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA   Shinozaki K.;
RT   "Analysis of multiple occurrences of alternative splicing events in
RT   Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL   DNA Res. 16:155-164(2009).
RN   [8]
RP   GENE FAMILY.
RX   PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA   Stracke R., Werber M., Weisshaar B.;
RT   "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL   Curr. Opin. Plant Biol. 4:447-456(2001).
RN   [9]
RP   DISRUPTION PHENOTYPE, FUNCTION, COMPONENT OF THE MAC COMPLEX, AND
RP   INTERACTION WITH PRL1 AND MOS4.
RX   PubMed=17575050; DOI=10.1101/gad.1559607;
RA   Palma K., Zhao Q., Cheng Y.T., Bi D., Monaghan J., Cheng W., Zhang Y.,
RA   Li X.;
RT   "Regulation of plant innate immunity by three proteins in a complex
RT   conserved across the plant and animal kingdoms.";
RL   Genes Dev. 21:1484-1493(2007).
RN   [10]
RP   DISRUPTION PHENOTYPE, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=17298883; DOI=10.1016/j.plaphy.2006.12.003;
RA   Lin Z., Yin K., Wang X., Liu M., Chen Z., Gu H., Qu L.J.;
RT   "Virus induced gene silencing of AtCDC5 results in accelerated cell death
RT   in Arabidopsis leaves.";
RL   Plant Physiol. Biochem. 45:87-94(2007).
RN   [11]
RP   IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF THE MAC COMPLEX, AND
RP   INTERACTION WITH PRP19A.
RX   PubMed=19629177; DOI=10.1371/journal.ppat.1000526;
RA   Monaghan J., Xu F., Gao M., Zhao Q., Palma K., Long C., Chen S., Zhang Y.,
RA   Li X.;
RT   "Two Prp19-like U-box proteins in the MOS4-associated complex play
RT   redundant roles in plant innate immunity.";
RL   PLoS Pathog. 5:E1000526-E1000526(2009).
RN   [12]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-343, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19376835; DOI=10.1104/pp.109.138677;
RA   Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA   Grossmann J., Gruissem W., Baginsky S.;
RT   "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT   chloroplast kinase substrates and phosphorylation networks.";
RL   Plant Physiol. 150:889-903(2009).
CC   -!- FUNCTION: Component of the MAC complex that probably regulates defense
CC       responses through transcriptional control and thereby is essential for
CC       plant innate immunity. Possesses a sequence specific DNA sequence
CC       'CTCAGCG' binding activity. Involved in mRNA splicing and cell cycle
CC       control. May also play a role in the response to DNA damage.
CC       {ECO:0000250|UniProtKB:Q99459, ECO:0000269|PubMed:17298883,
CC       ECO:0000269|PubMed:17575050, ECO:0000269|PubMed:8917598}.
CC   -!- SUBUNIT: Component of the multiprotein assembly MOS4-associated complex
CC       (MAC) at least composed of MOS4, CDC5, PRL1 and PRP19. Interacts with
CC       PRL1, MOS4 and PRP19A. Associated with the spliceosome.
CC       {ECO:0000269|PubMed:17575050, ECO:0000269|PubMed:19629177}.
CC   -!- INTERACTION:
CC       P92948; Q949S9: MOS4; NbExp=3; IntAct=EBI-1382948, EBI-1382943;
CC       P92948; Q42384: PRL1; NbExp=2; IntAct=EBI-1382948, EBI-1382964;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC       ECO:0000269|PubMed:17298883}.
CC   -!- TISSUE SPECIFICITY: Expressed extensively in shoot and root meristems.
CC       {ECO:0000269|PubMed:8917598}.
CC   -!- DISRUPTION PHENOTYPE: Accelerated cell death. Enhanced susceptibility
CC       to virulent and avirulent pathogens. {ECO:0000269|PubMed:17298883,
CC       ECO:0000269|PubMed:17575050}.
CC   -!- SIMILARITY: Belongs to the CEF1 family. {ECO:0000305}.
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DR   EMBL; D58424; BAA09598.1; -; mRNA.
DR   EMBL; AY519553; AAS10023.1; -; mRNA.
DR   EMBL; AC000132; AAB60730.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE28490.1; -; Genomic_DNA.
DR   EMBL; OZ408683; CAO7026736.1; -; Genomic_DNA.
DR   EMBL; AY093057; AAM13056.1; -; mRNA.
DR   EMBL; BT008801; AAP68240.1; -; mRNA.
DR   EMBL; AK316890; BAH19597.1; -; mRNA.
DR   PIR; F86231; F86231.
DR   RefSeq; NP_172448.1; NM_100849.3.
DR   AlphaFoldDB; P92948; -.
DR   SMR; P92948; -.
DR   BioGRID; 22747; 25.
DR   FunCoup; P92948; 4646.
DR   IntAct; P92948; 7.
DR   STRING; 3702.P92948; -.
DR   GlyGen; P92948; 1 site.
DR   iPTMnet; P92948; -.
DR   PaxDb; 3702-P92948; -.
DR   ProteomicsDB; 222807; -.
DR   GeneID; 837506; -.
DR   KEGG; ath:AT1G09770; -.
DR   Araport; AT1G09770; -.
DR   TAIR; AT1G09770; CDC5.
DR   eggNOG; KOG0050; Eukaryota.
DR   HOGENOM; CLU_009082_1_0_1; -.
DR   InParanoid; P92948; -.
DR   OMA; CVAMGNE; -.
DR   PhylomeDB; P92948; -.
DR   CD-CODE; 4299E36E; Nucleolus.
DR   PRO; PR:P92948; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   GO; GO:0005576; C:extracellular region; HDA:TAIR.
DR   GO; GO:0005634; C:nucleus; EXP:UniProtKB.
DR   GO; GO:0000974; C:Prp19 complex; IBA:GO_Central.
DR   GO; GO:0005681; C:spliceosomal complex; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0042742; P:defense response to bacterium; IMP:TAIR.
DR   GO; GO:0050832; P:defense response to fungus; IMP:TAIR.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of DNA-templated transcription; TAS:TAIR.
DR   CDD; cd00167; SANT; 1.
DR   CDD; cd11659; SANT_CDC5_II; 1.
DR   FunFam; 1.10.10.60:FF:000021; CDC5 cell division cycle 5-like; 1.
DR   FunFam; 1.10.10.60:FF:000091; CDC5 cell division cycle 5-like; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 2.
DR   InterPro; IPR047242; CDC5L/Cef1.
DR   InterPro; IPR021786; Cdc5p/Cef1_C.
DR   InterPro; IPR009057; Homeodomain-like_sf.
DR   InterPro; IPR017930; Myb_dom.
DR   InterPro; IPR001005; SANT/Myb.
DR   InterPro; IPR047240; SANT_CDC5L_II.
DR   PANTHER; PTHR45885; CELL DIVISION CYCLE 5-LIKE PROTEIN; 1.
DR   PANTHER; PTHR45885:SF1; CELL DIVISION CYCLE 5-LIKE PROTEIN; 1.
DR   Pfam; PF11831; Myb_Cef; 1.
DR   Pfam; PF13921; Myb_DNA-bind_6; 1.
DR   SMART; SM00717; SANT; 2.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS51294; HTH_MYB; 2.
PE   1: Evidence at protein level;
KW   Activator; Cell cycle; Coiled coil; DNA damage; DNA repair; DNA-binding;
KW   Immunity; Innate immunity; mRNA processing; mRNA splicing; Nucleus;
KW   Phosphoprotein; Plant defense; Reference proteome; Repeat; Spliceosome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..844
FT                   /note="Cell division cycle 5-like protein"
FT                   /id="PRO_0000391630"
FT   DOMAIN          2..57
FT                   /note="HTH myb-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   DOMAIN          58..107
FT                   /note="HTH myb-type 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   DNA_BIND        30..53
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   DNA_BIND        81..103
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT   REGION          113..147
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          379..514
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          145..189
FT                   /evidence="ECO:0000255"
FT   COILED          685..723
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        113..126
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        406..416
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        433..442
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        446..475
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        500..513
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         343
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:19376835"
FT   CONFLICT        177
FT                   /note="E -> K (in Ref. 2; AAS10023)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        460..461
FT                   /note="HE -> QQ (in Ref. 1; BAA09598)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        476
FT                   /note="S -> G (in Ref. 7; BAH19597)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   844 AA;  95767 MW;  B640967BB8AB4614 CRC64;
     MRIMIKGGVW KNTEDEILKA AVMKYGKNQW ARISSLLVRK SAKQCKARWY EWLDPSIKKT
     EWTREEDEKL LHLAKLLPTQ WRTIAPIVGR TPSQCLERYE KLLDAACTKD ENYDAADDPR
     KLRPGEIDPN PEAKPARPDP VDMDEDEKEM LSEARARLAN TRGKKAKRKA REKQLEEARR
     LASLQKRREL KAAGIDGRHR KRKRKGIDYN AEIPFEKRAP AGFYDTADED RPADQVKFPT
     TIEELEGKRR ADVEAHLRKQ DVARNKIAQR QDAPAAILQA NKLNDPEVVR KRSKLMLPPP
     QISDHELEEI AKMGYASDLL AENEELTEGS AATRALLANY SQTPRQGMTP MRTPQRTPAG
     KGDAIMMEAE NLARLRDSQT PLLGGENPEL HPSDFTGVTP RKKEIQTPNP MLTPSMTPGG
     AGLTPRIGLT PSRDGSSFSM TPKGTPFRDE LHINEDMDMH ESAKLERQRR EEARRSLRSG
     LTGLPQPKNE YQIVAQPPPE ESEEPEEKIE EDMSDRIARE KAEEEARQQA LLKKRSKVLQ
     RDLPRPPAAS LAVIRNSLLS ADGDKSSVVP PTPIEVADKM VREELLQLLE HDNAKYPLDD
     KAEKKKGAKN RTNRSASQVL AIDDFDENEL QEADKMIKEE GKFLCVSMGH ENKTLDDFVE
     AHNTCVNDLM YFPTRSAYEL SSVAGNADKV AAFQEEMENV RKKMEEDEKK AEHMKAKYKT
     YTKGHERRAE TVWTQIEATL KQAEIGGTEV ECFKALKRQE EMAASFRKKN LQEEVIKQKE
     TESKLQTRYG NMLAMVEKAE EIMVGFRAQA LKKQEDVEDS HKLKEAKLAT GEEEDIAIAM
     EASA
//