ID   RIP_PLETG               Reviewed;          28 AA.
AC   P83245;
DT   10-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   02-SEP-2026, entry version 40.
DE   RecName: Full=Ribosome-inactivating protein pleuturegin;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Fragment;
OS   Pleurotus tuber-regium (King tuber oyster mushroom) (Lentinus
OS   tuber-regium).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Pleurotineae; Pleurotaceae; Pleurotus.
OX   NCBI_TaxID=716892;
RN   [1]
RP   PROTEIN SEQUENCE, AND FUNCTION.
RC   TISSUE=Sclerotium;
RX   PubMed=11676502; DOI=10.1006/bbrc.2001.5816;
RA   Wang H.X., Ng T.B.;
RT   "Isolation of pleuturegin, a novel ribosome-inactivating protein from fresh
RT   sclerotia of the edible mushroom Pleurotus tuber-regium.";
RL   Biochem. Biophys. Res. Commun. 288:718-721(2001).
CC   -!- FUNCTION: Inhibits protein synthesis in animal cells. Does not possess
CC       ribonuclease activity. {ECO:0000269|PubMed:11676502}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of the N-glycosidic bond at one specific
CC         adenosine on the 28S rRNA.; EC=3.2.2.22;
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P83245; -.
DR   GO; GO:0030598; F:rRNA N-glycosylase activity; IDA:UniProtKB.
DR   GO; GO:0017148; P:negative regulation of translation; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Hydrolase; Protein synthesis inhibitor; Toxin.
FT   CHAIN           1..>28
FT                   /note="Ribosome-inactivating protein pleuturegin"
FT                   /id="PRO_0000221421"
FT   NON_TER         28
SQ   SEQUENCE   28 AA;  2961 MW;  49FCAB47D3DBC98A CRC64;
     ARTQPGNIAP VGDFTLYPNA PRQGHIVA
//