ID   HC3L_ACIFR              Reviewed;         122 AA.
AC   P80509;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   09-APR-2025, entry version 51.
DE   RecName: Full=Cytochrome c3 hydrogenase large chain;
DE            Short=Hydrogenase;
DE            EC=1.12.2.1;
DE   Flags: Fragments;
GN   Name=hoxG;
OS   Acidithiobacillus ferrooxidans (Thiobacillus ferrooxidans).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Acidithiobacillia;
OC   Acidithiobacillales; Acidithiobacillaceae; Acidithiobacillus.
OX   NCBI_TaxID=920;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=ATCC 19859 / BCRC 13033 / JCM 3863 / NCIMB 9490;
RX   PubMed=8661919; DOI=10.1007/s002030050329;
RA   Fischer J., Quentmeier A., Kostka S., Kraft R., Friedrich C.G.;
RT   "Purification and characterization of the hydrogenase from Thiobacillus
RT   ferrooxidans.";
RL   Arch. Microbiol. 165:289-296(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 Fe(III)-[cytochrome c3] + H2 = 2 Fe(II)-[cytochrome c3] + 2
CC         H(+); Xref=Rhea:RHEA:20625, Rhea:RHEA-COMP:11576, Rhea:RHEA-
CC         COMP:11577, ChEBI:CHEBI:15378, ChEBI:CHEBI:18276, ChEBI:CHEBI:29033,
CC         ChEBI:CHEBI:29034; EC=1.12.2.1;
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC   -!- CAUTION: The order of the peptides shown is uncertain. It is also
CC       unknown if gaps exist between the peptides. {ECO:0000305}.
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DR   GO; GO:0047806; F:cytochrome-c3 hydrogenase activity; IEA:UniProtKB-EC.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Iron; Oxidoreductase.
FT   CHAIN           1..>122
FT                   /note="Cytochrome c3 hydrogenase large chain"
FT                   /id="PRO_0000083913"
FT   NON_CONS        20..21
FT                   /evidence="ECO:0000305"
FT   NON_CONS        29..30
FT                   /evidence="ECO:0000305"
FT   NON_CONS        35..36
FT                   /evidence="ECO:0000305"
FT   NON_CONS        42..43
FT                   /evidence="ECO:0000305"
FT   NON_CONS        59..60
FT                   /evidence="ECO:0000305"
FT   NON_CONS        72..73
FT                   /evidence="ECO:0000305"
FT   NON_CONS        78..79
FT                   /evidence="ECO:0000305"
FT   NON_CONS        87..88
FT                   /evidence="ECO:0000305"
FT   NON_CONS        98..99
FT                   /evidence="ECO:0000305"
FT   NON_CONS        107..108
FT                   /evidence="ECO:0000305"
FT   NON_TER         122
SQ   SEQUENCE   122 AA;  13152 MW;  EB4FAF365348DA0B CRC64;
     XTAVQTLDIS PVGRVEGDLD SXSILEYFRN AILARFGGGL GKYAPFTGTN YEIGVTISGD
     KDPQAGLVVT PRESTTXGLN EPFYIKPYVS XGYIPHEDGA LAHXIEVDGV VVEAYTQAEL
     FR
//