ID   NDUS3_SOLTU             Reviewed;         190 AA.
AC   P80261;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2003, sequence version 3.
DT   02-SEP-2026, entry version 117.
DE   RecName: Full=NADH dehydrogenase [ubiquinone] iron-sulfur protein 3;
DE            EC=7.1.1.2;
DE   AltName: Full=Complex I-27kD;
DE            Short=CI-27kD;
DE   AltName: Full=NADH dehydrogenase subunit 9;
DE   AltName: Full=NADH-ubiquinone oxidoreductase 27 kDa subunit;
GN   Name=NAD9;
OS   Solanum tuberosum (Potato).
OG   Mitochondrion.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE, AND RNA
RP   EDITING.
RC   TISSUE=Tuber;
RX   PubMed=8078764; DOI=10.1093/nar/22.16.3304;
RA   Grohmann L., Thieck O., Herz U., Schroeder W., Brennicke A.;
RT   "Translation of nad9 mRNAs in mitochondria from Solanum tuberosum is
RT   restricted to completely edited transcripts.";
RL   Nucleic Acids Res. 22:3304-3311(1994).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-17.
RC   STRAIN=cv. Bintje; TISSUE=Tuber;
RX   PubMed=8294484; DOI=10.1016/s0021-9258(17)42163-6;
RA   Herz U., Schroeder W., Liddell A., Leaver C.J., Brennicke A., Grohmann L.;
RT   "Purification of the NADH:ubiquinone oxidoreductase (complex I) of the
RT   respiratory chain from the inner mitochondrial membrane of Solanum
RT   tuberosum.";
RL   J. Biol. Chem. 269:2263-2269(1994).
CC   -!- FUNCTION: Core subunit of the mitochondrial membrane respiratory chain
CC       NADH dehydrogenase (Complex I) that is believed to belong to the
CC       minimal assembly required for catalysis. Complex I functions in the
CC       transfer of electrons from NADH to the respiratory chain. The immediate
CC       electron acceptor for the enzyme is believed to be ubiquinone (By
CC       similarity). {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + NADH + 5 H(+)(in) = a ubiquinol + NAD(+) + 4
CC         H(+)(out); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBUNIT: Complex I is composed of about 45 different subunits. This is
CC       a component of the iron-sulfur (IP) fragment of the enzyme (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane; Peripheral membrane
CC       protein; Matrix side.
CC   -!- RNA EDITING: Modified_positions=31 {ECO:0000269|PubMed:8078764}, 56
CC       {ECO:0000269|PubMed:8078764}, 100 {ECO:0000269|PubMed:8078764}, 110
CC       {ECO:0000269|PubMed:8078764}, 122 {ECO:0000269|PubMed:8078764}, 133
CC       {ECO:0000269|PubMed:8078764}, 147 {ECO:0000269|PubMed:8078764};
CC   -!- SIMILARITY: Belongs to the complex I 30 kDa subunit family.
CC       {ECO:0000305}.
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DR   EMBL; X79774; CAA56168.1; ALT_SEQ; Genomic_DNA.
DR   PIR; S48062; S48062.
DR   AlphaFoldDB; P80261; -.
DR   SMR; P80261; -.
DR   FunCoup; P80261; 1760.
DR   STRING; 4113.P80261; -.
DR   PaxDb; 4113-P80261; -.
DR   InParanoid; P80261; -.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0045271; C:respiratory chain complex I; IBA:GO_Central.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   FunFam; 3.30.460.80:FF:000005; NADH dehydrogenase subunit 9; 1.
DR   Gene3D; 3.30.460.80; NADH:ubiquinone oxidoreductase, 30kDa subunit; 1.
DR   HAMAP; MF_01357; NDH1_NuoC; 1.
DR   InterPro; IPR010218; NADH_DH_suC.
DR   InterPro; IPR037232; NADH_quin_OxRdtase_su_C/D-like.
DR   InterPro; IPR001268; NADH_UbQ_OxRdtase_30kDa_su.
DR   InterPro; IPR020396; NADH_UbQ_OxRdtase_CS.
DR   NCBIfam; TIGR01961; NuoC_fam; 1.
DR   NCBIfam; NF004733; PRK06074.1-5; 1.
DR   PANTHER; PTHR10884:SF14; NADH DEHYDROGENASE [UBIQUINONE] IRON-SULFUR PROTEIN 3, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR10884; NADH DEHYDROGENASE UBIQUINONE IRON-SULFUR PROTEIN 3; 1.
DR   Pfam; PF00329; Complex1_30kDa; 1.
DR   SUPFAM; SSF143243; Nqo5-like; 1.
DR   PROSITE; PS00542; COMPLEX1_30K; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Electron transport; Membrane; Mitochondrion;
KW   Mitochondrion inner membrane; NAD; Oxidoreductase; Respiratory chain;
KW   RNA editing; Translocase; Transport; Ubiquinone.
FT   CHAIN           1..190
FT                   /note="NADH dehydrogenase [ubiquinone] iron-sulfur protein
FT                   3"
FT                   /id="PRO_0000118643"
SQ   SEQUENCE   190 AA;  22883 MW;  A150CF461B493A53 CRC64;
     MDNQFIFKYS WETLPKKWVK KMERSEHGNR FDTNTDYLFQ LLCFMKLHTY TRVQVLIDIC
     GVDYPSRKQR FEVVYNLLSI RYNSRIRVQT SADEVTRISS VVSLFPSAGW WEREVWDMFG
     VFSINHPDLR RILTDYGFEG HPLRKDFPLS GYVEVRYDDP EKRVVSEPIE MTQEFRYFDF
     ASPWEQRSDG
//