ID   HYFG_ECOLI              Reviewed;         555 AA.
AC   P77329; P76974;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   02-SEP-2026, entry version 171.
DE   RecName: Full=Hydrogenase-4 component G;
DE            EC=1.-.-.-;
GN   Name=hyfG {ECO:0000303|PubMed:9387241};
GN   OrderedLocusNames=b2487, JW2472;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND POSSIBLE FUNCTION.
RC   STRAIN=K12;
RX   PubMed=9387241; DOI=10.1099/00221287-143-11-3633;
RA   Andrews S.C., Berks B.C., McClay J., Ambler A., Quail M.A., Golby P.,
RA   Guest J.R.;
RT   "A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-
RT   translocating formate hydrogenlyase system.";
RL   Microbiology 143:3633-3647(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA   Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA   Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA   Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT   genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT   its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Possible component of hydrogenase 4.
CC       {ECO:0000305|PubMed:9387241}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC       Note=Binds 1 [4Fe-4S] cluster.;
CC   -!- INTERACTION:
CC       P77329; P0A6F5: groEL; NbExp=4; IntAct=EBI-548413, EBI-543750;
CC   -!- SIMILARITY: Belongs to the complex I 49 kDa subunit family.
CC       {ECO:0000305}.
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DR   EMBL; M63654; AAB88569.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75540.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA16375.2; -; Genomic_DNA.
DR   PIR; F65024; F65024.
DR   RefSeq; NP_416982.1; NC_000913.3.
DR   RefSeq; WP_001102321.1; NZ_CP009789.1.
DR   AlphaFoldDB; P77329; -.
DR   SMR; P77329; -.
DR   BioGRID; 4260765; 7.
DR   ComplexPortal; CPX-6028; Formate hydrogenlyase-H/Hydrogenase-4 complex.
DR   DIP; DIP-9989N; -.
DR   FunCoup; P77329; 80.
DR   IntAct; P77329; 7.
DR   STRING; 511145.b2487; -.
DR   TCDB; 3.D.1.9.1; the h+ or na+-translocating nadh dehydrogenase (ndh) family.
DR   PaxDb; 511145-b2487; -.
DR   EnsemblBacteria; AAC75540; AAC75540; b2487.
DR   GeneID; 946964; -.
DR   KEGG; ecj:JW2472; -.
DR   KEGG; eco:b2487; -.
DR   KEGG; ecoc:C3026_13800; -.
DR   PATRIC; fig|1411691.4.peg.4252; -.
DR   EchoBASE; EB3967; -.
DR   eggNOG; COG3261; Bacteria.
DR   eggNOG; COG3262; Bacteria.
DR   HOGENOM; CLU_015134_3_1_6; -.
DR   InParanoid; P77329; -.
DR   OMA; VGIVEGW; -.
DR   OrthoDB; 9801496at2; -.
DR   PhylomeDB; P77329; -.
DR   BioCyc; EcoCyc:MONOMER0-150; -.
DR   PRO; PR:P77329; -.
DR   Proteomes; UP000000625; Chromosome.
DR   AbasyAtlas; P77329; -.
DR   GO; GO:0009326; C:formate dehydrogenase complex; NAS:ComplexPortal.
DR   GO; GO:0016020; C:membrane; NAS:ComplexPortal.
DR   GO; GO:0051540; F:metal cluster binding; ISM:EcoCyc.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0048038; F:quinone binding; IEA:InterPro.
DR   GO; GO:0019645; P:anaerobic electron transport chain; IBA:GO_Central.
DR   GO; GO:0009061; P:anaerobic respiration; NAS:ComplexPortal.
DR   GO; GO:0015944; P:formate oxidation; NAS:ComplexPortal.
DR   GO; GO:0006007; P:glucose catabolic process; NAS:ComplexPortal.
DR   FunFam; 1.10.645.10:FF:000004; Hydrogenase 3, large subunit; 1.
DR   Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR   Gene3D; 3.30.460.80; NADH:ubiquinone oxidoreductase, 30kDa subunit; 1.
DR   InterPro; IPR052197; ComplexI_49kDa-like.
DR   InterPro; IPR001135; NADH_Q_OxRdtase_suD.
DR   InterPro; IPR037232; NADH_quin_OxRdtase_su_C/D-like.
DR   InterPro; IPR001268; NADH_UbQ_OxRdtase_30kDa_su.
DR   InterPro; IPR014029; NADH_UbQ_OxRdtase_49kDa_CS.
DR   InterPro; IPR001501; Ni-dep_hyd_lsu.
DR   InterPro; IPR029014; NiFe-Hase_large.
DR   PANTHER; PTHR43485:SF1; FORMATE HYDROGENLYASE SUBUNIT 5-RELATED; 1.
DR   PANTHER; PTHR43485; HYDROGENASE-4 COMPONENT G; 1.
DR   Pfam; PF00329; Complex1_30kDa; 1.
DR   Pfam; PF00346; Complex1_49kDa; 1.
DR   Pfam; PF00374; NiFeSe_Hases; 1.
DR   SUPFAM; SSF56762; HydB/Nqo4-like; 1.
DR   SUPFAM; SSF143243; Nqo5-like; 1.
DR   PROSITE; PS00535; COMPLEX1_49K; 1.
PE   1: Evidence at protein level;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; NAD; Oxidoreductase;
KW   Reference proteome.
FT   CHAIN           1..555
FT                   /note="Hydrogenase-4 component G"
FT                   /id="PRO_0000118626"
SQ   SEQUENCE   555 AA;  63383 MW;  A5D410E6E74C05CD CRC64;
     MNVNSSSNRG EAILAALKTQ FPGAVLDEER QTPEQVTITV KINLLPDVVQ YLYYQHDGWL
     PVLFGNDERT LNGHYAVYYA LSMEGAEKCW IVVKALVDAD SREFPSVTPR VPAAVWGERE
     IRDMYGLIPV GLPDQRRLVL PDDWPEDMHP LRKDAMDYRL RPEPTTDSET YPFINEGNSD
     ARVIPVGPLH ITSDEPGHFR LFVDGEQIVD ADYRLFYVHR GMEKLAETRM GYNEVTFLSD
     RVCGICGFAH SVAYTNSVEN ALGIEVPQRA HTIRSILLEV ERLHSHLLNL GLSCHFVGFD
     TGFMQFFRVR EKSMTMAELL IGSRKTYGLN LIGGVRRDIL KEQRLQTLKL VREMRADVSE
     LVEMLLATPN MEQRTQGIGI LDRQIARDLR FDHPYADYGN IPKTLFTFTG GDVFSRVMVR
     VKETFDSLAM LEFALDNMPD TPLLTEGFSY KPHAFALGFV EAPRGEDVHW SMLGDNQKLF
     RWRCRAATYA NWPVLRYMLR GNTVSDAPLI IGSLDPCYSC TDRVTLVDVR KRQSKTVPYK
     EIERYGIDRN RSPLK
//