ID   MDLA_ECOLI              Reviewed;         590 AA.
AC   P77265; P30751; Q2MBX8;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   02-SEP-2026, entry version 190.
DE   RecName: Full=Multidrug resistance-like ATP-binding protein MdlA;
DE            EC=7.6.2.2;
GN   Name=mdlA; Synonyms=mdl; OrderedLocusNames=b0448, JW0438;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=TAP90 / ATCC 47037;
RX   PubMed=7904973; DOI=10.1016/0378-1119(93)90470-n;
RA   Allikmets R., Gerrard B.C., Court D., Dean M.C.;
RT   "Cloning and organization of the abc and mdl genes of Escherichia coli:
RT   relationship to eukaryotic multidrug resistance.";
RL   Gene 136:231-236(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RA   Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M.,
RA   Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D.,
RA   Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.;
RT   "Sequence of minutes 4-25 of Escherichia coli.";
RL   Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + xenobioticSide 1 = ADP + phosphate +
CC         xenobioticSide 2.; EC=7.6.2.2;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:15919996}; Multi-pass membrane protein
CC       {ECO:0000255|PROSITE-ProRule:PRU00441, ECO:0000269|PubMed:15919996}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. Drug exporter-2
CC       (TC 3.A.1.117) family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC36870.1; Type=Frameshift; Note=Fuses together mdlA and mdlB.; Evidence={ECO:0000305};
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DR   EMBL; L08627; AAC36870.1; ALT_FRAME; Unassigned_DNA.
DR   EMBL; U82664; AAB40204.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73551.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76228.1; -; Genomic_DNA.
DR   PIR; H64774; H64774.
DR   RefSeq; NP_414982.1; NC_000913.3.
DR   RefSeq; WP_001235649.1; NZ_CP009789.1.
DR   AlphaFoldDB; P77265; -.
DR   SMR; P77265; -.
DR   BioGRID; 4261677; 158.
DR   DIP; DIP-10173N; -.
DR   FunCoup; P77265; 298.
DR   IntAct; P77265; 1.
DR   STRING; 511145.b0448; -.
DR   TCDB; 3.A.1.106.13; the atp-binding cassette (abc) superfamily.
DR   PaxDb; 511145-b0448; -.
DR   EnsemblBacteria; AAC73551; AAC73551; b0448.
DR   GeneID; 945092; -.
DR   KEGG; ecj:JW0438; -.
DR   KEGG; eco:b0448; -.
DR   KEGG; ecoc:C3026_02195; -.
DR   PATRIC; fig|1411691.4.peg.1828; -.
DR   EchoBASE; EB1579; -.
DR   eggNOG; COG1132; Bacteria.
DR   HOGENOM; CLU_000604_84_6_6; -.
DR   InParanoid; P77265; -.
DR   OMA; CRLYEPQ; -.
DR   OrthoDB; 9806127at2; -.
DR   PhylomeDB; P77265; -.
DR   BioCyc; EcoCyc:MDLA-MONOMER; -.
DR   PRO; PR:P77265; -.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016020; C:membrane; ISM:EcoCyc.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0008559; F:ABC-type xenobiotic transporter activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; ISM:EcoCyc.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR   GO; GO:0046618; P:xenobiotic export from cell; IC:EcoCyc.
DR   CDD; cd18541; ABC_6TM_TmrB_like; 1.
DR   FunFam; 1.20.1560.10:FF:000011; Multidrug ABC transporter ATP-binding protein; 1.
DR   FunFam; 3.40.50.300:FF:000221; Multidrug ABC transporter ATP-binding protein; 1.
DR   Gene3D; 1.20.1560.10; ABC transporter type 1, transmembrane domain; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR011527; ABC1_TM_dom.
DR   InterPro; IPR036640; ABC1_TM_sf.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR039421; Type_1_exporter.
DR   NCBIfam; NF008055; PRK10789.1; 1.
DR   PANTHER; PTHR43394:SF1; ATP-BINDING CASSETTE SUB-FAMILY B MEMBER 10, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43394; ATP-DEPENDENT PERMEASE MDL1, MITOCHONDRIAL; 1.
DR   Pfam; PF00664; ABC_membrane; 1.
DR   Pfam; PF00005; ABC_tran; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF90123; ABC transporter transmembrane region; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS50929; ABC_TM1F; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW   Nucleotide-binding; Reference proteome; Translocase; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..590
FT                   /note="Multidrug resistance-like ATP-binding protein MdlA"
FT                   /id="PRO_0000092492"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        134..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        155..175
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        248..268
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        280..300
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   DOMAIN          18..303
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   DOMAIN          337..570
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   BINDING         369..376
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   CONFLICT        145
FT                   /note="G -> A (in Ref. 1; AAC36870)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        234..235
FT                   /note="NM -> KL (in Ref. 1; AAC36870)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        318
FT                   /note="A -> R (in Ref. 1; AAC36870)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        371
FT                   /note="T -> N (in Ref. 1; AAC36870)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        390
FT                   /note="E -> P (in Ref. 1; AAC36870)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        410..411
FT                   /note="SR -> TG (in Ref. 1; AAC36870)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   590 AA;  66017 MW;  AD1CEBE41030200D CRC64;
     MRLFAQLSWY FRREWRRYLG AVALLVIIAM LQLVPPKVVG IVVDGVTEQH FTTGQILMWI
     ATMVLIAVVV YLLRYVWRVL LFGASYQLAV ELREDYYRQL SRQHPEFYLR HRTGDLMARA
     TNDVDRVVFA AGEGVLTLVD SLVMGCAVLI MMSTQISWQL TLFSLLPMPV MAIMIKRNGD
     ALHERFKLAQ AAFSSLNDRT QESLTSIRMI KAFGLEDRQS ALFAADAEDT GKKNMRVARI
     DARFDPTIYI AIGMANLLAI GGGSWMVVQG SLTLGQLTSF MMYLGLMIWP MLALAWMFNI
     VERGSAAYSR IRAMLAEAPV VNDGSEPVPE GRGELDVNIH QFTYPQTDHP ALENVNFALK
     PGQMLGICGP TGSGKSTLLS LIQRHFDVSE GDIRFHDIPL TKLQLDSWRS RLAVVSQTPF
     LFSDTVANNI ALGCPNATQQ EIEHVARLAS VHDDILRLPQ GYDTEVGERG VMLSGGQKQR
     ISIARALLVN AEILILDDAL SAVDGRTEHQ ILHNLRQWGQ GRTVIISAHR LSALTEASEI
     IVMQHGHIAQ RGNHDVLAQQ SGWYRDMYRY QQLEAALDDA PENREEAVDA
//