ID   YFCU_ECOLI              Reviewed;         881 AA.
AC   P77196; P77532;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 3.
DT   18-JUN-2025, entry version 159.
DE   RecName: Full=Putative outer membrane usher protein YfcU;
DE   Flags: Precursor;
GN   Name=yfcU; Synonyms=yfcT; OrderedLocusNames=b4661, JW2334/JW2335;
GN   ORFNames=b2337, b2338;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA   Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA   Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA   Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT   genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT   its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=20345943; DOI=10.1111/j.1462-2920.2010.02202.x;
RA   Korea C.G., Badouraly R., Prevost M.C., Ghigo J.M., Beloin C.;
RT   "Escherichia coli K-12 possesses multiple cryptic but functional chaperone-
RT   usher fimbriae with distinct surface specificities.";
RL   Environ. Microbiol. 12:1957-1977(2010).
CC   -!- FUNCTION: Part of the yfcOPQRSUV fimbrial operon. Could contribute to
CC       adhesion to various surfaces in specific environmental niches.
CC       Increases adhesion to eukaryotic T24 bladder epithelial cells in the
CC       absence of fim genes. Probably involved in the export and assembly of
CC       fimbrial subunits across the outer membrane.
CC       {ECO:0000269|PubMed:20345943}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- INDUCTION: Expression is negatively regulated by H-NS and subjected to
CC       cAMP receptor protein (CRP)-mediated catabolite repression.
CC       {ECO:0000269|PubMed:20345943}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the operon under classical laboratory
CC       conditions does not result in any major effect on E.coli capacity to
CC       form biofilms compared with the wild-type strain.
CC       {ECO:0000269|PubMed:20345943}.
CC   -!- MISCELLANEOUS: The operon is cryptic under classical laboratory
CC       conditions, but is functional when constitutively expressed.
CC       {ECO:0000305|PubMed:20345943}.
CC   -!- SIMILARITY: Belongs to the fimbrial export usher family. {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. This sequence is
CC       interrupted by a stop codon. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA16191.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAA16192.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
CC       Sequence=U00096; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP009048; BAA16191.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AP009048; BAA16192.1; ALT_SEQ; Genomic_DNA.
DR   PIR; G65006; G65006.
DR   PIR; H65006; H65006.
DR   AlphaFoldDB; P77196; -.
DR   SMR; P77196; -.
DR   BioGRID; 4261776; 11.
DR   BioGRID; 4262083; 227.
DR   DIP; DIP-11989N; -.
DR   FunCoup; P77196; 188.
DR   IntAct; P77196; 20.
DR   KEGG; ecj:JW2334; -.
DR   KEGG; ecj:JW2335; -.
DR   EchoBASE; EB3876; -.
DR   eggNOG; COG3188; Bacteria.
DR   HOGENOM; CLU_009120_2_2_6; -.
DR   InParanoid; P77196; -.
DR   PhylomeDB; P77196; -.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IBA:GO_Central.
DR   GO; GO:0015473; F:fimbrial usher porin activity; IBA:GO_Central.
DR   GO; GO:0009297; P:pilus assembly; IBA:GO_Central.
DR   Gene3D; 2.60.40.2070; -; 1.
DR   Gene3D; 2.60.40.3110; -; 1.
DR   Gene3D; 3.10.20.410; -; 1.
DR   Gene3D; 2.60.40.2610; Outer membrane usher protein FimD, plug domain; 1.
DR   InterPro; IPR000015; Fimb_usher.
DR   InterPro; IPR018030; Fimbrial_membr_usher_CS.
DR   InterPro; IPR042186; FimD_plug_dom.
DR   InterPro; IPR025949; PapC-like_C.
DR   InterPro; IPR043142; PapC-like_C_sf.
DR   InterPro; IPR025885; PapC_N.
DR   InterPro; IPR037224; PapC_N_sf.
DR   NCBIfam; NF011812; PRK15284.1; 1.
DR   PANTHER; PTHR30451; OUTER MEMBRANE USHER PROTEIN; 1.
DR   PANTHER; PTHR30451:SF10; OUTER MEMBRANE USHER PROTEIN YFCU-RELATED; 1.
DR   Pfam; PF13953; PapC_C; 1.
DR   Pfam; PF13954; PapC_N; 1.
DR   Pfam; PF00577; Usher; 1.
DR   SUPFAM; SSF141729; FimD N-terminal domain-like; 1.
DR   PROSITE; PS01151; FIMBRIAL_USHER; 1.
PE   5: Uncertain;
KW   Cell outer membrane; Fimbrium biogenesis; Membrane; Reference proteome;
KW   Signal; Transmembrane; Transmembrane beta strand; Transport.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..881
FT                   /note="Putative outer membrane usher protein YfcU"
FT                   /id="PRO_0000009332"
SQ   SEQUENCE   881 AA;  97490 MW;  48B392C1AE2C9111 CRC64;
     MPDHSLFRLR ILPWCIALAM SGSYSSVWAE DDIQFDSRFL ELKGDTKIDL KRFSSQGYVE
     PGKYNLQVQL NKQPLAEEYD IYWYAGEDDV SKSYACLTPE LVAQFGLKED VAKNLQWSHD
     GKCLKPGQLE GVEIKADLSQ SALVISLPQA YLEYTWPDWD PPSRWDDGIS GIIADYSITA
     QTRHEENGGD DSNEISGNGT VGVNLGPWRM RADWQTNYQH TRSNDDDDEF GGDDTQKKWE
     WSRYYAWRAL PSLKAKLALG EDYLNSDIFD GFNYVGGSVS TDDQMLPPNL RGYAPDISGV
     AHTTAKVTVS QMGRVIYETQ VPAGPFRIQD LGDSVSGTLH IRIEEQNGQV QEYDISTASM
     PYLTRPGQVR YKIMMGRPQE WGHHVEGGFF SGAEASWGIA NGWSLYGGAL GDENYQSAAL
     GVGRDLSTFG AVAFDVTHSH TKLDKDTAYG KGSLDGNSFR VSYSKDFDQL NSRVTFAGYR
     FSEENFMTMS EYLDASDSEM VRTGNDKEMY TATYNQNFRD AGVSVYLNYT RHTYWDREEQ
     TNYNIMLSHY FNMGSIRNMS VSLTGYRYEY DNRADKGMYI SLSMPWGDNS TVSYNGNYGS
     GTDSSQVGYF SRVDDATHYQ LNIGTSDKHT SVDGYYSHDG SLAQVDLSAN YHEGQYTSAG
     LSLQGGATLT THGGALHRTQ NMGGTRLLID ADGVADVPVE GNGAAVYTNM FGKAVVSDVN
     NYYRNQAYID LNKLPENAEA TQSVVQATLT EGAIGYRKFA VISGQKAMAV LRLQDGSHPP
     FGAEVKNDNE QTVGLVDDDG SVYLAGVKPG EHMSVFWSGV AHCDINLPDP LPADLFNGLL
     LPCQHKGNVA PVVPDDIKPV IQEQTQQVTP TDPPVSVSAN Q
//