ID   SIXA_ECOLI              Reviewed;         161 AA.
AC   P76502; P77784;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   10-JUN-2026, entry version 169.
DE   RecName: Full=Phosphohistidine phosphatase SixA;
DE            EC=3.1.3.-;
DE   AltName: Full=RX6;
GN   Name=sixA; Synonyms=yfcW; OrderedLocusNames=b2340, JW2337;
OS   Escherichia coli (strain K12).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=9489669; DOI=10.1046/j.1365-2958.1998.00703.x;
RA   Ogino H., Matsubara M., Kato N., Nakamura Y., Mizuno T.;
RT   "An Escherichia coli protein that exhibits phosphohistidine phosphatase
RT   activity towards the HPt domain of the ArcB sensor involved in the
RT   multistep His-Asp phosphorelay.";
RL   Mol. Microbiol. 27:573-585(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA   Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA   Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA   Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT   genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT   its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO 80.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Exhibits phosphohistidine phosphatase activity towards the
CC       HPt domain of the ArcB sensor involved in the multistep His-Asp
CC       phosphorelay.
CC   -!- INTERACTION:
CC       P76502; Q2EES9: torI; NbExp=3; IntAct=EBI-548621, EBI-9154838;
CC   -!- SIMILARITY: Belongs to the SixA phosphatase family. {ECO:0000305}.
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DR   EMBL; D86298; BAA24878.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75400.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA16194.2; -; Genomic_DNA.
DR   PIR; B65007; B65007.
DR   RefSeq; NP_416842.1; NC_000913.3.
DR   RefSeq; WP_001195819.1; NZ_CP009789.1.
DR   PDB; 1UJB; X-ray; 2.06 A; A=1-161.
DR   PDB; 1UJC; X-ray; 1.90 A; A=1-161.
DR   AlphaFoldDB; P76502; -.
DR   SMR; P76502; -.
DR   BioGRID; 4260532; 16.
DR   BioGRID; 851156; 8.
DR   DIP; DIP-10885N; -.
DR   FunCoup; P76502; 150.
DR   IntAct; P76502; 10.
DR   STRING; 511145.b2340; -.
DR   jPOST; P76502; -.
DR   PaxDb; 511145-b2340; -.
DR   EnsemblBacteria; AAC75400; AAC75400; b2340.
DR   GeneID; 93774836; -.
DR   GeneID; 946815; -.
DR   KEGG; ecj:JW2337; -.
DR   KEGG; eco:b2340; -.
DR   KEGG; ecoc:C3026_13025; -.
DR   PATRIC; fig|1411691.4.peg.4392; -.
DR   EchoBASE; EB3878; -.
DR   eggNOG; COG2062; Bacteria.
DR   HOGENOM; CLU_084603_1_1_6; -.
DR   InParanoid; P76502; -.
DR   OMA; MQVLIMR; -.
DR   OrthoDB; 92610at2; -.
DR   PhylomeDB; P76502; -.
DR   BioCyc; EcoCyc:G7211-MONOMER; -.
DR   BRENDA; 3.9.1.3; 2026.
DR   EvolutionaryTrace; P76502; -.
DR   PRO; PR:P76502; -.
DR   Proteomes; UP000000625; Chromosome.
DR   AbasyAtlas; P76502; -.
DR   GO; GO:0005737; C:cytoplasm; IDA:EcoliWiki.
DR   GO; GO:0101006; F:protein histidine phosphatase activity; IDA:EcoliWiki.
DR   GO; GO:0035971; P:peptidyl-histidine dephosphorylation; IDA:EcoCyc.
DR   GO; GO:0070297; P:regulation of phosphorelay signal transduction system; IMP:EcoCyc.
DR   CDD; cd07067; HP_PGM_like; 1.
DR   FunFam; 3.40.50.1240:FF:000004; Phosphohistidine phosphatase SixA; 1.
DR   Gene3D; 3.40.50.1240; Phosphoglycerate mutase-like; 1.
DR   InterPro; IPR013078; His_Pase_superF_clade-1.
DR   InterPro; IPR029033; His_PPase_superfam.
DR   InterPro; IPR004449; SixA.
DR   NCBIfam; TIGR00249; sixA; 1.
DR   Pfam; PF00300; His_Phos_1; 1.
DR   SUPFAM; SSF53254; Phosphoglycerate mutase-like; 1.
DR   PDBsum; 1UJB; -.
DR   PDBsum; 1UJC; -.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase; Protein phosphatase; Reference proteome.
FT   CHAIN           1..161
FT                   /note="Phosphohistidine phosphatase SixA"
FT                   /id="PRO_0000214568"
FT   CONFLICT        80
FT                   /note="T -> K (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   STRAND          2..7
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   HELIX           8..10
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   STRAND          15..17
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   HELIX           18..20
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   HELIX           25..40
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   STRAND          47..50
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   HELIX           54..66
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   HELIX           77..79
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   HELIX           85..98
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   STRAND          102..107
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   HELIX           111..119
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   HELIX           123..127
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   STRAND          133..138
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   STRAND          144..150
FT                   /evidence="ECO:0007829|PDB:1UJC"
FT   HELIX           152..154
FT                   /evidence="ECO:0007829|PDB:1UJC"
SQ   SEQUENCE   161 AA;  17208 MW;  98D9470A4EBAAA57 CRC64;
     MQVFIMRHGD AALDAASDSV RPLTTNGCDE SRLMANWLKG QKVEIERVLV SPFLRAEQTL
     EEVGDCLNLP SSAEVLPELT PCGDVGLVSA YLQALTNEGV ASVLVISHLP LVGYLVAELC
     PGETPPMFTT SAIASVTLDE SGNGTFNWQM SPCNLKMAKA I
//