ID   P41_MYCPN               Reviewed;         357 AA.
AC   P75470; O08089;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   04-NOV-2026, entry version 85.
DE   RecName: Full=Wheel complex protein P41 {ECO:0000305|PubMed:26633540};
DE   AltName: Full=Cytoskeletal protein P41 {ECO:0000303|PubMed:17163973};
GN   OrderedLocusNames=MPN_311; ORFNames=F10_orf357, MP525;
OS   Mycoplasmoides pneumoniae (strain ATCC 29342 / M129 / Subtype 1)
OS   (Mycoplasma pneumoniae).
OC   Bacteria; Bacillati; Mycoplasmatota; Mycoplasmoidales; Mycoplasmoidaceae;
OC   Mycoplasmoides.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129 / Subtype 1;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=ATCC 29342 / M129 / Subtype 1;
RX   PubMed=9098066; DOI=10.1128/jb.179.8.2668-2677.1997;
RA   Krause D.C., Proft T., Hedreyda C.T., Hilbert H., Plagens H., Herrmann R.;
RT   "Transposon mutagenesis reinforces the correlation between Mycoplasma
RT   pneumoniae cytoskeletal protein HMW2 and cytadherence.";
RL   J. Bacteriol. 179:2668-2677(1997).
RN   [3]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 29342 / M129 / Subtype 1;
RX   PubMed=15466048; DOI=10.1128/jb.186.20.6944-6955.2004;
RA   Kenri T., Seto S., Horino A., Sasaki Y., Sasaki T., Miyata M.;
RT   "Use of fluorescent-protein tagging to determine the subcellular
RT   localization of mycoplasma pneumoniae proteins encoded by the cytadherence
RT   regulatory locus.";
RL   J. Bacteriol. 186:6944-6955(2004).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 29342 / M129 / Subtype 1;
RX   PubMed=17163973; DOI=10.1111/j.1365-2958.2006.05507.x;
RA   Hasselbring B.M., Krause D.C.;
RT   "Cytoskeletal protein P41 is required to anchor the terminal organelle of
RT   the wall-less prokaryote Mycoplasma pneumoniae.";
RL   Mol. Microbiol. 63:44-53(2007).
RN   [5]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 29342 / M129 / Subtype 1;
RX   PubMed=17693502; DOI=10.1128/jb.00867-07;
RA   Hasselbring B.M., Krause D.C.;
RT   "Proteins P24 and P41 function in the regulation of terminal-organelle
RT   development and gliding motility in Mycoplasma pneumoniae.";
RL   J. Bacteriol. 189:7442-7449(2007).
RN   [6]
RP   FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 29342 / M129 / Subtype 1;
RX   PubMed=26633540; DOI=10.1371/journal.ppat.1005299;
RA   Nakane D., Kenri T., Matsuo L., Miyata M.;
RT   "Systematic Structural Analyses of Attachment Organelle in Mycoplasma
RT   pneumoniae.";
RL   PLoS Pathog. 11:e1005299-e1005299(2015).
CC   -!- FUNCTION: Stably anchors the attachment organelle to the cell body and
CC       for their spatial positioning (PubMed:17163973, PubMed:17693502).
CC       Required for stable accumulation of P28 (also called HMW2-s, a short
CC       protein produced by upstream MPN_310) (PubMed:17163973). Required for
CC       correct localization of P24 (PubMed:17693502). Ectopic expression of
CC       P41 restores gliding velocity but not gliding frequency to a P41-P24
CC       disruption, attachment organelles do not initiate as frequently as in
CC       wild-type; inclusion of P24 restores a wild-type phenotype
CC       (PubMed:17693502). May play a role in attachment organelle initiation
CC       and its coordination with the cell cycle (Probable) (PubMed:17163973,
CC       PubMed:17693502). Component of the wheel complex, part of the
CC       cytoskeleton-like structure in the attachment organelle which
CC       stabilizes the shape of the wall-less Mycoplasma (PubMed:26633540). The
CC       attachment organelle is involved in gliding cell motility
CC       (PubMed:17163973) and adhesion to host cells.
CC       {ECO:0000269|PubMed:17163973, ECO:0000269|PubMed:17693502,
CC       ECO:0000269|PubMed:26633540, ECO:0000305|PubMed:17163973,
CC       ECO:0000305|PubMed:17693502}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:Q9ZB78}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, attachment organelle
CC       {ECO:0000269|PubMed:15466048, ECO:0000269|PubMed:17693502,
CC       ECO:0000269|PubMed:26633540}. Cytoplasm, cytoskeleton
CC       {ECO:0000269|PubMed:26633540}. Note=Localizes to the proximal end of
CC       the attachment organelle, behind HMW2 and P65 (PubMed:15466048,
CC       PubMed:17693502). With MPN_387, P200, TopJ, P24 and possibly Lon
CC       protease, forms the wheel (or bowl) complex in the attachment organelle
CC       (PubMed:26633540). {ECO:0000269|PubMed:15466048,
CC       ECO:0000269|PubMed:17693502, ECO:0000269|PubMed:26633540}.
CC   -!- INDUCTION: Part of the crl operon (cytadherence regulatory locus) with
CC       MPN_309 (P65), MPN_310 (HMW2 and P28/HMW2-s), MPN_311 (P41) and MPN_312
CC       (P24). {ECO:0000305|PubMed:9098066}.
CC   -!- DISRUPTION PHENOTYPE: Decreased gliding movement, loss of expression of
CC       protein P41 and downstream protein P24, decreased accumulation of P28
CC       (also called HMW2-s, a short protein produced by upstream HMW2). Cells
CC       are coccoid chains rather than filamentous, and form many additional,
CC       DNA-free small cell fragments that are detached attachment organelles
CC       (with P1, P30 and P65 proteins). Terminal organelle formation often
CC       initiates away from cell poles. Complementation by P41 alone, but not
CC       by P24, restores wild-type cell morphology and stability to the
CC       terminal organelle (but not wild-type levels of P28), but does not
CC       restore wild-type gliding frequency. {ECO:0000269|PubMed:17163973}.
CC   -!- SIMILARITY: Belongs to the P41 protein family. {ECO:0000305}.
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DR   EMBL; U00089; AAB96173.1; -; Genomic_DNA.
DR   EMBL; U59896; AAB52528.1; -; Genomic_DNA.
DR   PIR; S73851; S73851.
DR   RefSeq; WP_010874667.1; NC_000912.1.
DR   AlphaFoldDB; P75470; -.
DR   SMR; P75470; -.
DR   IntAct; P75470; 2.
DR   STRING; 272634.MPN_311; -.
DR   PaxDb; 272634-MPN_311; -.
DR   EnsemblBacteria; AAB96173; AAB96173; MPN_311.
DR   KEGG; mpn:MPN_311; -.
DR   PATRIC; fig|272634.6.peg.335; -.
DR   HOGENOM; CLU_775731_0_0_14; -.
DR   OMA; FQGNFPK; -.
DR   OrthoDB; 9960111at2; -.
DR   BioCyc; MPNE272634:G1GJ3-497-MONOMER; -.
DR   Proteomes; UP000000808; Chromosome.
DR   GO; GO:0033099; C:attachment organelle; IEA:UniProtKB-SubCell.
DR   GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR   InterPro; IPR055086; MG491_central.
DR   Pfam; PF22373; MG491_central; 1.
PE   1: Evidence at protein level;
KW   Cell projection; Cytadherence; Cytoplasm; Cytoskeleton; Reference proteome;
KW   Virulence.
FT   CHAIN           1..357
FT                   /note="Wheel complex protein P41"
FT                   /id="PRO_0000210461"
SQ   SEQUENCE   357 AA;  40580 MW;  F828443341D3D7F9 CRC64;
     MTNDYQQLNY LVETDDEADI IIANLVKQLN ELKEILLSLD NQDLEINQVN HRTTVHNTSS
     NTSNNSTSNN IADGNYNSNF FHNFSKETLQ TQAKRGFLLL ERCSLVGLQQ LELEYLNVLG
     RSFESHQDKI NFLVNLRDLT NDHLLDTEKI VSTLEQIFNV IGGTEYTPIL NSFFNQSLND
     PDPIQREIGL KQFVANLRQR FKTLLQKTNN SIQQLEAEIQ IPTTHIKSDE VMFGPPDMNE
     RLVLNDSETD AILRSIEAEL ESALQNKKQV VVTAVPPIAA NLATDSISQE TLEHNLNNTE
     TVNTTTVTVT SASETQVRKP QISLRPIFQG NFPKRLSRED IQRYAHQLQE LEQSNEG
//