ID   T1R3_MYCPN              Reviewed;         206 AA.
AC   P75433;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   02-SEP-2026, entry version 116.
DE   RecName: Full=Putative type I restriction enzyme MpnIIP endonuclease subunit C-terminal part {ECO:0000303|PubMed:12654995, ECO:0000303|PubMed:8948633};
DE            Short=R protein C-terminal part;
DE   AltName: Full=Putative type-1 restriction enzyme MpnORFDP R protein part 3;
DE            Short=MpnORFDBP;
GN   OrderedLocusNames=MPN_345; ORFNames=H91_orf206, MP491;
OS   Mycoplasmoides pneumoniae (strain ATCC 29342 / M129 / Subtype 1)
OS   (Mycoplasma pneumoniae).
OC   Bacteria; Bacillati; Mycoplasmatota; Mycoplasmoidales; Mycoplasmoidaceae;
OC   Mycoplasmoides.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129 / Subtype 1;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 15531 / FH / type 2;
RX   PubMed=20543037; DOI=10.1128/aem.00024-10;
RA   Krishnakumar R., Assad-Garcia N., Benders G.A., Phan Q., Montague M.G.,
RA   Glass J.I.;
RT   "Targeted chromosomal knockouts in Mycoplasma pneumoniae.";
RL   Appl. Environ. Microbiol. 76:5297-5299(2010).
RN   [4]
RP   DISCUSSION OF SEQUENCE.
RC   STRAIN=ATCC 29342 / M129 / Subtype 1;
RX   PubMed=23300489; DOI=10.1371/journal.pgen.1003191;
RA   Lluch-Senar M., Luong K., Llorens-Rico V., Delgado J., Fang G., Spittle K.,
RA   Clark T.A., Schadt E., Turner S.W., Korlach J., Serrano L.;
RT   "Comprehensive methylome characterization of Mycoplasma genitalium and
RT   Mycoplasma pneumoniae at single-base resolution.";
RL   PLoS Genet. 9:e1003191-e1003191(2013).
CC   -!- FUNCTION: The C-terminal section of a putative type I restriction
CC       enzyme that if reconstituted might recognize 5'-GAN(7)TAY-3' and cleave
CC       a random distance away (Probable). Subunit R is required for both
CC       nuclease and ATPase activities, but not for modification
CC       (PubMed:12654995). {ECO:0000303|PubMed:12654995,
CC       ECO:0000305|PubMed:8948633}.
CC   -!- DISRUPTION PHENOTYPE: Non-essential, it can be deleted.
CC       {ECO:0000269|PubMed:20543037}.
CC   -!- CAUTION: Could be the product of a pseudogene; in this organism the
CC       endonuclease subunit carries 2 frameshift mutations (resulting in 3
CC       short ORFs) and is probably not expressed.
CC       {ECO:0000305|PubMed:23300489, ECO:0000305|PubMed:8948633}.
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DR   EMBL; U00089; AAB96139.1; -; Genomic_DNA.
DR   PIR; S73817; S73817.
DR   AlphaFoldDB; P75433; -.
DR   SMR; P75433; -.
DR   STRING; 272634.MPN_345; -.
DR   PaxDb; 272634-MPN_345; -.
DR   EnsemblBacteria; AAB96139; AAB96139; MPN_345.
DR   KEGG; mpn:MPN_345; -.
DR   HOGENOM; CLU_004848_5_0_14; -.
DR   PRO; PR:P75433; -.
DR   Proteomes; UP000000808; Chromosome.
DR   Gene3D; 3.90.1570.50; -; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR040980; SWI2_SNF2.
DR   InterPro; IPR051268; Type-I_R_enzyme_R_subunit.
DR   PANTHER; PTHR30195:SF16; TYPE I RESTRICTION ENZYME ENDONUCLEASE SUBUNIT; 1.
DR   PANTHER; PTHR30195; TYPE I SITE-SPECIFIC DEOXYRIBONUCLEASE PROTEIN SUBUNIT M AND R; 1.
DR   Pfam; PF18766; SWI2_SNF2; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE   5: Uncertain;
KW   Reference proteome; Restriction system.
FT   CHAIN           1..206
FT                   /note="Putative type I restriction enzyme MpnIIP
FT                   endonuclease subunit C-terminal part"
FT                   /id="PRO_0000077264"
SQ   SEQUENCE   206 AA;  23957 MW;  E69EB18CF7185F20 CRC64;
     MRWSDSENNK IDYIEDFATH FLNKNALLNV ICKFCVFRSN SDLWVMRPYQ ICATERILEK
     IKEDNRNSKN SKNASKGGCI WHSTGSGKTL TSFKAVQLAS EIDFVDKVLF VVDRKDLDNQ
     TIEEYEKFQA GSVSETENTN DLKEKILDDS TATRAIVTTI HKLKRLIDQR SKLKDEDLKK
     KNIVLIFDEC HRSQFGKMKQ EIDEFF
//