ID   RLUD_BORBU              Reviewed;         326 AA.
AC   P70870; O51050;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   02-SEP-2026, entry version 115.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase D {ECO:0000250|UniProtKB:P33643};
DE            EC=5.4.99.23 {ECO:0000250|UniProtKB:P33643};
DE   AltName: Full=23S rRNA pseudouridine(1911/1915/1917) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase D;
DE   AltName: Full=rRNA-uridine isomerase D;
GN   Name=rluD {ECO:0000250|UniProtKB:P33643};
GN   Synonyms=yfiI {ECO:0000303|Ref.1}; OrderedLocusNames=BB_0018;
OS   Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS   (Borrelia burgdorferi).
OC   Bacteria; Pseudomonadati; Spirochaetota; Spirochaetia; Spirochaetales;
OC   Borreliaceae; Borreliella.
OX   NCBI_TaxID=224326;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=HB19;
RA   Boursaux-Eude C., Margarita D., Belfaiza J., Old I.G., Saint-Girons I.;
RT   "Homologues of helicase genes, priA and ruvAB in the left telomeric region
RT   of the linear chromosome of Borrelia burgdorferi.";
RL   Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=9403685; DOI=10.1038/37551;
RA   Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA   Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA   Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA   Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA   van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA   Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA   Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA   Smith H.O., Venter J.C.;
RT   "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL   Nature 390:580-586(1997).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC       positions 1911, 1915 and 1917 in 23S ribosomal RNA.
CC       {ECO:0000250|UniProtKB:P33643}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(1911/1915/1917) in 23S rRNA =
CC         pseudouridine(1911/1915/1917) in 23S rRNA; Xref=Rhea:RHEA:42524,
CC         Rhea:RHEA-COMP:10097, Rhea:RHEA-COMP:10098, ChEBI:CHEBI:65314,
CC         ChEBI:CHEBI:65315; EC=5.4.99.23;
CC         Evidence={ECO:0000250|UniProtKB:P33643};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P33643}.
CC       Note=Associates with late stage pre-50S ribosomal subunits.
CC       {ECO:0000250|UniProtKB:P33643}.
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
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DR   EMBL; Y09142; CAA70352.1; -; Genomic_DNA.
DR   EMBL; AE000783; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; B70102; B70102.
DR   RefSeq; WP_002658364.1; NC_001318.1.
DR   AlphaFoldDB; P70870; -.
DR   SMR; P70870; -.
DR   GeneID; 46847259; -.
DR   Proteomes; UP000001807; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0160140; F:23S rRNA pseudouridine(1911/1915/1917) synthase activity; ISS:UniProtKB.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:TreeGrafter.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR050188; RluA_PseudoU_synthase.
DR   NCBIfam; TIGR00005; rluA_subfam; 1.
DR   PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR   PANTHER; PTHR21600:SF44; RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE D; 1.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Isomerase; Reference proteome; rRNA processing.
FT   CHAIN           1..326
FT                   /note="Ribosomal large subunit pseudouridine synthase D"
FT                   /id="PRO_0000162683"
FT   ACT_SITE        144
FT                   /evidence="ECO:0000250"
FT   CONFLICT        172
FT                   /note="T -> I (in Ref. 1; CAA70352)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        268
FT                   /note="K -> E (in Ref. 1; CAA70352)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        299..326
FT                   /note="FVIFLSNFYKSDELNLIIDNLVLFLRDF -> ICYFFVKFLQE (in Ref.
FT                   1; CAA70352)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   326 AA;  38067 MW;  52266C0AF8BFEA3C CRC64;
     MIRVKREFTV KENALRLDLY LSGNFEVFTR SQIKRRNVEA FKKSNGKFLK IKLSKPVFKD
     DEILIEFDEE SSQIDCLRPS NIPIAIIYED SNVIVLNKPQ GILSHPGISH WDDTVVNFLL
     YHIKSLKINF NEEKIRPGIV HRLDKDTSGV LICAKNISTL RFLAQQFKDK RTNKVYIAIV
     KGNFNSFFGS IESFIDRDKH NRKKFSVSKD RGKKALTEYR LLLNFGEYSL LALKPKTGRT
     HQLRVHMKYL NFPILGDEVY GRVDGSLKKI TLMLHSYKLE IDVGKNSFKK FISEFPKRFV
     IFLSNFYKSD ELNLIIDNLV LFLRDF
//