ID   HES5_MOUSE              Reviewed;         167 AA.
AC   P70120; Q8BVI1;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   04-NOV-2026, entry version 188.
DE   RecName: Full=Transcription factor HES-5;
DE   AltName: Full=Hairy and enhancer of split 5;
GN   Name=Hes5; Synonyms=Hes-5;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/Sv; TISSUE=Liver;
RX   PubMed=7836401; DOI=10.1074/jbc.270.3.1342;
RA   Takebayashi K., Akazawa C., Nakanishi S., Kageyama R.;
RT   "Structure and promoter analysis of the gene encoding the mouse helix-loop-
RT   helix factor HES-5. Identification of the neural precursor cell-specific
RT   promoter element.";
RL   J. Biol. Chem. 270:1342-1349(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Eye, and Olfactory bulb;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   FUNCTION AS NEUROGENESIS REPRESSOR.
RX   PubMed=23160044; DOI=10.1038/nn.3264;
RA   Tiberi L., van den Ameele J., Dimidschstein J., Piccirilli J., Gall D.,
RA   Herpoel A., Bilheu A., Bonnefont J., Iacovino M., Kyba M., Bouschet T.,
RA   Vanderhaeghen P.;
RT   "BCL6 controls neurogenesis through Sirt1-dependent epigenetic repression
RT   of selective Notch targets.";
RL   Nat. Neurosci. 15:1627-1635(2012).
CC   -!- FUNCTION: Transcriptional repressor of genes that require a bHLH
CC       protein for their transcription. Plays an important role as
CC       neurogenesis negative regulator. {ECO:0000269|PubMed:23160044}.
CC   -!- SUBUNIT: Transcription repression requires formation of a complex with
CC       a corepressor protein of the Groucho/TLE family. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- DOMAIN: Has a particular type of basic domain (presence of a helix-
CC       interrupting proline) that binds to the N-box (CACNAG), rather than the
CC       canonical E-box (CANNTG).
CC   -!- DOMAIN: The C-terminal WRPW motif is a transcriptional repression
CC       domain necessary for the interaction with Groucho/TLE family members,
CC       transcriptional corepressors recruited to specific target DNA by Hairy-
CC       related proteins. {ECO:0000250}.
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DR   EMBL; D32132; BAA06858.1; -; Genomic_DNA.
DR   EMBL; AK078170; BAC37159.1; -; mRNA.
DR   EMBL; AK087508; BAC39904.1; -; mRNA.
DR   CCDS; CCDS19018.1; -.
DR   PIR; A55438; A55438.
DR   RefSeq; NP_034549.1; NM_010419.4.
DR   AlphaFoldDB; P70120; -.
DR   SMR; P70120; -.
DR   BioGRID; 200278; 2.
DR   ELM; P70120; -.
DR   FunCoup; P70120; 1236.
DR   STRING; 10090.ENSMUSP00000051118; -.
DR   iPTMnet; P70120; -.
DR   PhosphoSitePlus; P70120; -.
DR   PaxDb; 10090-ENSMUSP00000051118; -.
DR   PeptideAtlas; P70120; -.
DR   Antibodypedia; 26785; 257 antibodies from 40 providers.
DR   DNASU; 15208; -.
DR   Ensembl; ENSMUST00000049621.7; ENSMUSP00000051118.7; ENSMUSG00000048001.8.
DR   GeneID; 15208; -.
DR   KEGG; mmu:15208; -.
DR   UCSC; uc008wck.1; mouse.
DR   AGR; MGI:104876; -.
DR   CTD; 388585; -.
DR   MGI; MGI:104876; Hes5.
DR   VEuPathDB; HostDB:ENSMUSG00000048001; -.
DR   eggNOG; ENOG502S6S1; Eukaryota.
DR   GeneTree; ENSGT00940000162836; -.
DR   HOGENOM; CLU_068550_3_1_1; -.
DR   InParanoid; P70120; -.
DR   OMA; APNAISM; -.
DR   OrthoDB; 6085656at2759; -.
DR   PhylomeDB; P70120; -.
DR   BioGRID-ORCS; 15208; 1 hit in 79 CRISPR screens.
DR   PRO; PR:P70120; -.
DR   Proteomes; UP000000589; Chromosome 4.
DR   RNAct; P70120; protein.
DR   Bgee; ENSMUSG00000048001; Expressed in medial ganglionic eminence and 160 other cell types or tissues.
DR   ExpressionAtlas; P70120; baseline and differential.
DR   GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003682; F:chromatin binding; ISO:MGI.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:UniProtKB.
DR   GO; GO:0003690; F:double-stranded DNA binding; ISO:GO_Central.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:GO_Central.
DR   GO; GO:0009952; P:anterior/posterior pattern specification; IBA:GO_Central.
DR   GO; GO:0048708; P:astrocyte differentiation; IDA:UniProtKB.
DR   GO; GO:0007420; P:brain development; IMP:UniProtKB.
DR   GO; GO:0043010; P:camera-type eye development; IMP:UniProtKB.
DR   GO; GO:0051216; P:cartilage development; IMP:UniProtKB.
DR   GO; GO:0007155; P:cell adhesion; IGI:MGI.
DR   GO; GO:0048469; P:cell maturation; IGI:MGI.
DR   GO; GO:0022010; P:central nervous system myelination; IMP:MGI.
DR   GO; GO:0072049; P:comma-shaped body morphogenesis; IEP:UniProtKB.
DR   GO; GO:0090162; P:establishment of epithelial cell polarity; IGI:MGI.
DR   GO; GO:0021861; P:forebrain radial glial cell differentiation; ISO:GO_Central.
DR   GO; GO:0021781; P:glial cell fate commitment; IMP:MGI.
DR   GO; GO:0060122; P:inner ear receptor cell stereocilium organization; IGI:MGI.
DR   GO; GO:0072282; P:metanephric nephron tubule morphogenesis; IEP:UniProtKB.
DR   GO; GO:0048712; P:negative regulation of astrocyte differentiation; IDA:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of DNA-templated transcription; IMP:UniProtKB.
DR   GO; GO:2000978; P:negative regulation of forebrain neuron differentiation; ISO:GO_Central.
DR   GO; GO:0045608; P:negative regulation of inner ear auditory receptor cell differentiation; IMP:MGI.
DR   GO; GO:2000981; P:negative regulation of inner ear receptor cell differentiation; IMP:UniProtKB.
DR   GO; GO:0045665; P:negative regulation of neuron differentiation; IMP:MGI.
DR   GO; GO:0048715; P:negative regulation of oligodendrocyte differentiation; IDA:UniProtKB.
DR   GO; GO:2000974; P:negative regulation of pro-B cell differentiation; ISS:UniProtKB.
DR   GO; GO:2000737; P:negative regulation of stem cell differentiation; ISS:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:MGI.
DR   GO; GO:0021915; P:neural tube development; IGI:MGI.
DR   GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR   GO; GO:0097150; P:neuronal stem cell population maintenance; IMP:UniProtKB.
DR   GO; GO:0007219; P:Notch signaling pathway; IDA:MGI.
DR   GO; GO:0014003; P:oligodendrocyte development; IMP:MGI.
DR   GO; GO:0030513; P:positive regulation of BMP signaling pathway; IGI:MGI.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; ISS:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of DNA-templated transcription; IMP:UniProtKB.
DR   GO; GO:0045747; P:positive regulation of Notch signaling pathway; IMP:UniProtKB.
DR   GO; GO:0046427; P:positive regulation of receptor signaling pathway via JAK-STAT; IDA:UniProtKB.
DR   GO; GO:0048661; P:positive regulation of smooth muscle cell proliferation; ISS:UniProtKB.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IGI:MGI.
DR   GO; GO:0065003; P:protein-containing complex assembly; IDA:UniProtKB.
DR   GO; GO:0045595; P:regulation of cell differentiation; IMP:MGI.
DR   GO; GO:0050678; P:regulation of epithelial cell proliferation; IGI:MGI.
DR   GO; GO:0031641; P:regulation of myelination; IMP:MGI.
DR   GO; GO:0050767; P:regulation of neurogenesis; IGI:MGI.
DR   GO; GO:0072050; P:S-shaped body morphogenesis; IEP:UniProtKB.
DR   GO; GO:0007224; P:smoothened signaling pathway; IDA:MGI.
DR   GO; GO:0072086; P:specification of loop of Henle identity; IEP:UniProtKB.
DR   GO; GO:0021537; P:telencephalon development; IGI:MGI.
DR   CDD; cd11461; bHLH-O_HES5; 1.
DR   FunFam; 4.10.280.10:FF:000033; Transcription factor HES-5; 1.
DR   Gene3D; 4.10.280.10; Helix-loop-helix DNA-binding domain; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR050370; HES_HEY.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR003650; Orange_dom.
DR   PANTHER; PTHR10985; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR, HES-RELATED; 1.
DR   Pfam; PF07527; Hairy_orange; 1.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SMART; SM00511; ORANGE; 1.
DR   SUPFAM; SSF47459; HLH, helix-loop-helix DNA-binding domain; 1.
DR   PROSITE; PS50888; BHLH; 1.
DR   PROSITE; PS51054; ORANGE; 1.
PE   1: Evidence at protein level;
KW   Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..167
FT                   /note="Transcription factor HES-5"
FT                   /id="PRO_0000127212"
FT   DOMAIN          16..72
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   DOMAIN          88..119
FT                   /note="Orange"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00380"
FT   REGION          124..167
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           164..167
FT                   /note="WRPW motif"
FT   COMPBIAS        124..138
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        139..160
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        22
FT                   /note="P -> S (in Ref. 2; BAC37159)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        94
FT                   /note="W -> L (in Ref. 2; BAC37159)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   167 AA;  18425 MW;  CA0E697094F4E760 CRC64;
     MAPSTVAVEM LSPKEKNRLR KPVVEKMRRD RINSSIEQLK LLLEQEFARH QPNSKLEKAD
     ILEMAVSYLK HSKAFAAAAG PKSLHQDYSE GYSWCLQEAV QFLTLHAASD TQMKLLYHFQ
     RPPAPAAPAK EPPAPGAAPQ PARSSAKAAA AAVSTSRQPA CGLWRPW
//