ID   MT_CHICK                Reviewed;          63 AA.
AC   P68497; P09576;
DT   23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   18-JUN-2025, entry version 99.
DE   RecName: Full=Metallothionein;
DE            Short=MT;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC   Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC   Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Liver;
RX   PubMed=2448773; DOI=10.1073/pnas.85.2.309;
RA   McCormick C.C., Fullmer C.S., Garvey J.S.;
RT   "Amino acid sequence and comparative antigenicity of chicken
RT   metallothionein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 85:309-313(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=White leghorn;
RX   PubMed=3340548; DOI=10.1093/nar/16.2.537;
RA   Wei D., Andrews G.K.;
RT   "Molecular cloning of chicken metallothionein. Deduction of the complete
RT   amino acid sequence and analysis of expression using cloned cDNA.";
RL   Nucleic Acids Res. 16:537-553(1988).
RN   [3]
RP   PROTEIN SEQUENCE.
RX   PubMed=2645390; DOI=10.1093/jn/119.2.309;
RA   Fernando L.P., Wei D., Andrews G.K.;
RT   "Structure and expression of chicken metallothionein.";
RL   J. Nutr. 119:309-318(1989).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2806910; DOI=10.1016/0378-1119(89)90349-1;
RA   Fernando L.P., Andrews G.K.;
RT   "Cloning and expression of an avian metallothionein-encoding gene.";
RL   Gene 81:177-183(1989).
CC   -!- FUNCTION: Metallothioneins have a high content of cysteine residues
CC       that bind various heavy metals.
CC   -!- DOMAIN: Class I metallothioneins contain 2 metal-binding domains: four
CC       divalent ions are chelated within cluster A of the alpha domain and are
CC       coordinated via cysteinyl thiolate bridges to 11 cysteine ligands.
CC       Cluster B, the corresponding region within the beta domain, can ligate
CC       three divalent ions to 9 cysteines.
CC   -!- SIMILARITY: Belongs to the metallothionein superfamily. Type 1 family.
CC       {ECO:0000305}.
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DR   EMBL; X06749; CAA29924.1; -; mRNA.
DR   EMBL; X13545; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A34958; A34958.
DR   RefSeq; NP_990606.1; NM_205275.2.
DR   AlphaFoldDB; P68497; -.
DR   SMR; P68497; -.
DR   FunCoup; P68497; 17.
DR   STRING; 9031.ENSGALP00000042113; -.
DR   PaxDb; 9031-ENSGALP00000042113; -.
DR   Ensembl; ENSGALT00010057893.1; ENSGALP00010035125.1; ENSGALG00010023744.1.
DR   GeneID; 396212; -.
DR   KEGG; gga:396212; -.
DR   CTD; 84560; -.
DR   VEuPathDB; HostDB:geneid_396212; -.
DR   eggNOG; KOG4738; Eukaryota.
DR   GeneTree; ENSGT00950000182967; -.
DR   HOGENOM; CLU_171204_2_0_1; -.
DR   InParanoid; P68497; -.
DR   OMA; KECKCSS; -.
DR   PhylomeDB; P68497; -.
DR   Reactome; R-GGA-5661231; Metallothioneins bind metals.
DR   PRO; PR:P68497; -.
DR   Proteomes; UP000000539; Chromosome 11.
DR   Bgee; ENSGALG00000028451; Expressed in kidney and 14 other cell types or tissues.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IBA:GO_Central.
DR   GO; GO:0008270; F:zinc ion binding; ISS:AgBase.
DR   GO; GO:0071276; P:cellular response to cadmium ion; IBA:GO_Central.
DR   GO; GO:0071280; P:cellular response to copper ion; IBA:GO_Central.
DR   GO; GO:0071294; P:cellular response to zinc ion; IBA:GO_Central.
DR   GO; GO:0010273; P:detoxification of copper ion; IBA:GO_Central.
DR   GO; GO:0006882; P:intracellular zinc ion homeostasis; IBA:GO_Central.
DR   GO; GO:0070555; P:response to interleukin-1; IDA:AgBase.
DR   GO; GO:0032496; P:response to lipopolysaccharide; ISS:AgBase.
DR   FunFam; 4.10.10.10:FF:000001; Metallothionein; 1.
DR   Gene3D; 4.10.10.10; Metallothionein Isoform II; 1.
DR   InterPro; IPR017854; Metalthion_dom_sf.
DR   InterPro; IPR023587; Metalthion_dom_sf_vert.
DR   InterPro; IPR000006; Metalthion_vert.
DR   InterPro; IPR018064; Metalthion_vert_metal_BS.
DR   PANTHER; PTHR23299; METALLOTHIONEIN; 1.
DR   PANTHER; PTHR23299:SF24; METALLOTHIONEIN-1X; 1.
DR   Pfam; PF00131; Metallothio; 1.
DR   PRINTS; PR00860; MTVERTEBRATE.
DR   SUPFAM; SSF57868; Metallothionein; 1.
DR   PROSITE; PS00203; METALLOTHIONEIN_VRT; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Metal-binding; Metal-thiolate cluster;
KW   Reference proteome; Zinc.
FT   CHAIN           1..63
FT                   /note="Metallothionein"
FT                   /id="PRO_0000197260"
FT   REGION          1..30
FT                   /note="Beta"
FT   REGION          31..63
FT                   /note="Alpha"
FT   BINDING         6
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /ligand_note="in cluster B"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         8
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /ligand_note="in cluster B"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         8
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /ligand_note="in cluster B"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         14
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /ligand_note="in cluster B"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         16
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /ligand_note="in cluster B"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         16
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="3"
FT                   /ligand_note="in cluster B"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         20
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="3"
FT                   /ligand_note="in cluster B"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         22
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /ligand_note="in cluster B"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         25
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="1"
FT                   /ligand_note="in cluster B"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         25
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="3"
FT                   /ligand_note="in cluster B"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         27
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="2"
FT                   /ligand_note="in cluster B"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         30
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="3"
FT                   /ligand_note="in cluster B"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         34
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="4"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         35
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="4"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         35
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="5"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         37
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="5"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         38
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="5"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         38
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="6"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         42
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="6"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         45
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="4"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         45
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="6"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         49
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="4"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         51
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="5"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         51
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="7"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         59
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="7"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         61
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="7"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         62
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="6"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
FT   BINDING         62
FT                   /ligand="a divalent metal cation"
FT                   /ligand_id="ChEBI:CHEBI:60240"
FT                   /ligand_label="7"
FT                   /ligand_note="in cluster A"
FT                   /evidence="ECO:0000250|UniProtKB:P02795"
SQ   SEQUENCE   63 AA;  6462 MW;  0062422501B9CC84 CRC64;
     MDPQDCTCAA GDSCSCAGSC KCKNCRCRSC RKSCCSCCPA GCNNCAKGCV CKEPASSKCS
     CCH
//