ID   QUEA_BRUME              Reviewed;         363 AA.
AC   P65948; Q8YHB3;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   02-SEP-2026, entry version 99.
DE   RecName: Full=S-adenosylmethionine:tRNA ribosyltransferase-isomerase {ECO:0000255|HAMAP-Rule:MF_00113};
DE            EC=2.4.99.17 {ECO:0000255|HAMAP-Rule:MF_00113};
DE   AltName: Full=Queuosine biosynthesis protein QueA {ECO:0000255|HAMAP-Rule:MF_00113};
GN   Name=queA {ECO:0000255|HAMAP-Rule:MF_00113};
GN   OrderedLocusNames=BMEI0889;
OS   Brucella melitensis biotype 1 (strain ATCC 23456 / CCUG 17765 / NCTC 10094
OS   / 16M).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
OC   Hyphomicrobiales; Brucellaceae; Brucella/Ochrobactrum group; Brucella;
OC   Brucella melitensis group.
OX   NCBI_TaxID=224914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23456 / CCUG 17765 / NCTC 10094 / 16M;
RX   PubMed=11756688; DOI=10.1073/pnas.221575398;
RA   DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA   Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA   Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA   Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA   Haselkorn R., Kyrpides N.C., Overbeek R.;
RT   "The genome sequence of the facultative intracellular pathogen Brucella
RT   melitensis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC   -!- FUNCTION: Transfers and isomerizes the ribose moiety from AdoMet to the
CC       7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give
CC       epoxyqueuosine (oQ-tRNA). {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=7-aminomethyl-7-carbaguanosine(34) in tRNA + S-adenosyl-L-
CC         methionine = epoxyqueuosine(34) in tRNA + adenine + L-methionine + 2
CC         H(+); Xref=Rhea:RHEA:32155, Rhea:RHEA-COMP:10342, Rhea:RHEA-
CC         COMP:18582, ChEBI:CHEBI:15378, ChEBI:CHEBI:16708, ChEBI:CHEBI:57844,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:82833, ChEBI:CHEBI:194443;
CC         EC=2.4.99.17; Evidence={ECO:0000255|HAMAP-Rule:MF_00113};
CC   -!- PATHWAY: tRNA modification; tRNA-queuosine biosynthesis.
CC       {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00113}.
CC   -!- SIMILARITY: Belongs to the QueA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00113}.
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DR   EMBL; AE008917; AAL52070.1; -; Genomic_DNA.
DR   PIR; AC3363; AC3363.
DR   RefSeq; WP_002964220.1; NC_003317.1.
DR   AlphaFoldDB; P65948; -.
DR   SMR; P65948; -.
DR   GeneID; 93016566; -.
DR   GeneID; 99643705; -.
DR   KEGG; bme:BMEI0889; -.
DR   KEGG; bmel:DK63_532; -.
DR   PATRIC; fig|224914.52.peg.555; -.
DR   eggNOG; COG0809; Bacteria.
DR   UniPathway; UPA00392; -.
DR   Proteomes; UP000000419; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051075; F:S-adenosylmethionine:tRNA ribosyltransferase-isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008616; P:tRNA queuosine(34) biosynthetic process; IEA:UniProtKB-UniRule.
DR   FunFam; 3.40.1780.10:FF:000001; S-adenosylmethionine:tRNA ribosyltransferase-isomerase; 1.
DR   Gene3D; 2.40.10.240; QueA-like; 1.
DR   Gene3D; 3.40.1780.10; QueA-like; 1.
DR   HAMAP; MF_00113; QueA; 1.
DR   InterPro; IPR003699; QueA.
DR   InterPro; IPR042118; QueA_dom1.
DR   InterPro; IPR042119; QueA_dom2.
DR   InterPro; IPR036100; QueA_sf.
DR   NCBIfam; NF001140; PRK00147.1; 1.
DR   NCBIfam; TIGR00113; queA; 1.
DR   PANTHER; PTHR30307; S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE; 1.
DR   PANTHER; PTHR30307:SF0; S-ADENOSYLMETHIONINE:TRNA RIBOSYLTRANSFERASE-ISOMERASE; 1.
DR   Pfam; PF02547; Queuosine_synth; 1.
DR   SUPFAM; SSF111337; QueA-like; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Queuosine biosynthesis; Reference proteome;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..363
FT                   /note="S-adenosylmethionine:tRNA ribosyltransferase-
FT                   isomerase"
FT                   /id="PRO_0000165386"
SQ   SEQUENCE   363 AA;  40174 MW;  972A658AA3E91CFD CRC64;
     MRVDLFDFDL PEERIALRPV EPRDHAKLLH VRPGEPFEDR HVYDLPDLLQ PGDALVFNDT
     KVIPAQLEGM RERTGNISQV SATLHMRVGP DRWKAFLRPA KRVKEGDRIR FGHSGTSCFL
     GTLDATVAEK GDSGEALLVF DLSGAVLDEA IAAVGHIPLP PYIASKRPED ERDRKDYQTV
     YAREEGAVAA PTAGLHFTPD LLEKIKARGI EEHFVTLHVG AGTFLPVKAD DTGDHKMHAE
     IGHVSQRTAS ALNAVHERGG RIICVGTTSL RLIESATGED GVVRPWSGAT DIFITPGYRF
     RAVDLLMTNF HLPRSTLFML VSAFSGLDTM HAAYNYAIAD GYRFYSYGDA SLLERIDHDR
     HSA
//