ID   PHS_BRUME               Reviewed;          97 AA.
AC   P65722; Q8YEL4;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   02-SEP-2026, entry version 95.
DE   RecName: Full=Putative pterin-4-alpha-carbinolamine dehydratase {ECO:0000255|HAMAP-Rule:MF_00434};
DE            Short=PHS {ECO:0000255|HAMAP-Rule:MF_00434};
DE            EC=4.2.1.96 {ECO:0000255|HAMAP-Rule:MF_00434};
DE   AltName: Full=4-alpha-hydroxy-tetrahydropterin dehydratase {ECO:0000255|HAMAP-Rule:MF_00434};
DE   AltName: Full=Pterin carbinolamine dehydratase {ECO:0000255|HAMAP-Rule:MF_00434};
DE            Short=PCD {ECO:0000255|HAMAP-Rule:MF_00434};
GN   Name=phhB; OrderedLocusNames=BMEI1864;
OS   Brucella melitensis biotype 1 (strain ATCC 23456 / CCUG 17765 / NCTC 10094
OS   / 16M).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
OC   Hyphomicrobiales; Brucellaceae; Brucella/Ochrobactrum group; Brucella;
OC   Brucella melitensis group.
OX   NCBI_TaxID=224914;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23456 / CCUG 17765 / NCTC 10094 / 16M;
RX   PubMed=11756688; DOI=10.1073/pnas.221575398;
RA   DelVecchio V.G., Kapatral V., Redkar R.J., Patra G., Mujer C., Los T.,
RA   Ivanova N., Anderson I., Bhattacharyya A., Lykidis A., Reznik G.,
RA   Jablonski L., Larsen N., D'Souza M., Bernal A., Mazur M., Goltsman E.,
RA   Selkov E., Elzer P.H., Hagius S., O'Callaghan D., Letesson J.-J.,
RA   Haselkorn R., Kyrpides N.C., Overbeek R.;
RT   "The genome sequence of the facultative intracellular pathogen Brucella
RT   melitensis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:443-448(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin =
CC         (6R)-L-erythro-6,7-dihydrobiopterin + H2O; Xref=Rhea:RHEA:11920,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15642, ChEBI:CHEBI:43120; EC=4.2.1.96;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00434};
CC   -!- SIMILARITY: Belongs to the pterin-4-alpha-carbinolamine dehydratase
CC       family. {ECO:0000255|HAMAP-Rule:MF_00434}.
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DR   EMBL; AE008917; AAL53045.1; -; Genomic_DNA.
DR   PIR; AB3485; AB3485.
DR   RefSeq; WP_002965330.1; NC_003317.1.
DR   PDB; 3JST; X-ray; 2.10 A; A/B=1-97.
DR   AlphaFoldDB; P65722; -.
DR   SMR; P65722; -.
DR   GeneID; 93017440; -.
DR   KEGG; bme:BMEI1864; -.
DR   KEGG; bmel:DK63_1625; -.
DR   PATRIC; fig|224914.52.peg.1713; -.
DR   eggNOG; COG2154; Bacteria.
DR   EvolutionaryTrace; P65722; -.
DR   Proteomes; UP000000419; Chromosome I.
DR   GO; GO:0008124; F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006729; P:tetrahydrobiopterin biosynthetic process; IEA:InterPro.
DR   CDD; cd00914; PCD_DCoH_subfamily_b; 1.
DR   Gene3D; 3.30.1360.20; Transcriptional coactivator/pterin dehydratase; 1.
DR   HAMAP; MF_00434; Pterin_4_alpha; 1.
DR   InterPro; IPR036428; PCD_sf.
DR   InterPro; IPR001533; Pterin_deHydtase.
DR   NCBIfam; NF002017; PRK00823.1-2; 1.
DR   NCBIfam; NF002018; PRK00823.1-3; 1.
DR   PANTHER; PTHR12599; PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE; 1.
DR   PANTHER; PTHR12599:SF0; PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE; 1.
DR   Pfam; PF01329; Pterin_4a; 1.
DR   SUPFAM; SSF55248; PCD-like; 1.
DR   PDBsum; 3JST; -.
PE   1: Evidence at protein level;
KW   3D-structure; Lyase; Reference proteome.
FT   CHAIN           1..97
FT                   /note="Putative pterin-4-alpha-carbinolamine dehydratase"
FT                   /id="PRO_0000063076"
FT   HELIX           5..7
FT                   /evidence="ECO:0007829|PDB:3JST"
FT   HELIX           8..16
FT                   /evidence="ECO:0007829|PDB:3JST"
FT   STRAND          30..35
FT                   /evidence="ECO:0007829|PDB:3JST"
FT   HELIX           39..56
FT                   /evidence="ECO:0007829|PDB:3JST"
FT   STRAND          61..65
FT                   /evidence="ECO:0007829|PDB:3JST"
FT   STRAND          68..73
FT                   /evidence="ECO:0007829|PDB:3JST"
FT   HELIX           76..78
FT                   /evidence="ECO:0007829|PDB:3JST"
FT   HELIX           83..96
FT                   /evidence="ECO:0007829|PDB:3JST"
SQ   SEQUENCE   97 AA;  11054 MW;  3176828807D408F3 CRC64;
     MARNRLTESE MNEALRALDG WQKVDGREAI TRSFKFKDFS TAFGFMAQAA LYAEKLDHHP
     EWFNAYNRVD VTLATHSENG VTELDIKMAR KMNAIAG
//