ID UBE2B_RAT Reviewed; 152 AA. AC P63149; P23567; Q9D0J6; DT 27-SEP-2004, integrated into UniProtKB/Swiss-Prot. DT 27-SEP-2004, sequence version 1. DT 02-SEP-2026, entry version 144. DE RecName: Full=Ubiquitin-conjugating enzyme E2 B {ECO:0000305}; DE EC=2.3.2.23 {ECO:0000250|UniProtKB:P63146}; DE AltName: Full=E2 ubiquitin-conjugating enzyme B; DE AltName: Full=RAD6 homolog B; DE Short=HR6B; DE AltName: Full=Ubiquitin carrier protein B; DE AltName: Full=Ubiquitin-conjugating enzyme E2(14k); DE AltName: Full=Ubiquitin-protein ligase B; GN Name=Ube2b {ECO:0000312|RGD:708345}; Synonyms=Rad6b; OS Rattus norvegicus (Rat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Rattus. OX NCBI_TaxID=10116; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION. RX PubMed=1313008; DOI=10.1016/s0021-9258(19)50455-0; RA Wing S.S., Dumas F., Banville D.; RT "A rabbit reticulocyte ubiquitin carrier protein that supports ubiquitin- RT dependent proteolysis (E214k) is homologous to the yeast DNA repair gene RT RAD6."; RL J. Biol. Chem. 267:6495-6501(1992). RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND TISSUE SPECIFICITY. RX PubMed=8048511; DOI=10.1152/ajpendo.1994.267.1.e39; RA Wing S.S., Banville D.; RT "14-kDa ubiquitin-conjugating enzyme: structure of the rat gene and RT regulation upon fasting and by insulin."; RL Am. J. Physiol. 267:E39-E48(1994). RN [3] RP NUCLEOTIDE SEQUENCE. RC TISSUE=Brain; RA Chen D., Graham S.H., Jing K., Simon R.P., Chen J.; RT "Isolation of a ubiquitin-conjugating enzyme gene induced following RT cerebral ischemia from the rat brain using modified subtractive RT hybridization and differential screening."; RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases. RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. RC TISSUE=Heart; RX PubMed=15489334; DOI=10.1101/gr.2596504; RG The MGC Project Team; RT "The status, quality, and expansion of the NIH full-length cDNA project: RT the Mammalian Gene Collection (MGC)."; RL Genome Res. 14:2121-2127(2004). RN [5] RP INDUCTION, SUBCELLULAR LOCATION, AND FUNCTION. RX PubMed=15946855; DOI=10.1016/j.mcn.2005.04.008; RA Kavakebi P., Hausott B., Tomasino A., Ingorokva S., Klimaschewski L.; RT "The N-end rule ubiquitin-conjugating enzyme, HR6B, is up-regulated by RT nerve growth factor and required for neurite outgrowth."; RL Mol. Cell. Neurosci. 29:559-568(2005). CC -!- FUNCTION: E2 ubiquitin-conjugating enzyme that accepts ubiquitin from CC the ubiquitin-activating enzyme E1 and transfers it to a E3 ubiquitin- CC protein ligase (PubMed:1313008). In vitro catalyzes 'Lys-11'-, as well CC as 'Lys-48'- and 'Lys-63'-linked polyubiquitination (By similarity). CC Together with the E3 enzyme BRE1 (RNF20 and/or RNF40), plays a role in CC transcription regulation by catalyzing the monoubiquitination of CC histone H2B at 'Lys-120' to form H2BK120ub1 (By similarity). H2BK120ub1 CC gives a specific tag for epigenetic transcriptional activation, CC elongation by RNA polymerase II, telomeric silencing, and is also a CC prerequisite for H3K4me and H3K79me formation (By similarity). May play CC a role in DNA repair (By similarity). Associates to the E3 ligase RAD18 CC to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono- CC ubiquitination of DNA-associated PCNA on 'Lys-164' (By similarity). In CC association with the E3 enzyme UBR4, is involved in N-end rule- CC dependent protein degradation (By similarity). May be involved in CC neurite outgrowth (PubMed:15946855). {ECO:0000250|UniProtKB:P63146, CC ECO:0000269|PubMed:1313008, ECO:0000269|PubMed:15946855}. CC -!- CATALYTIC ACTIVITY: CC Reaction=S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + CC [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin- CC activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin- CC conjugating enzyme]-L-cysteine.; EC=2.3.2.23; CC Evidence={ECO:0000250|UniProtKB:P63146, ECO:0000255|PROSITE- CC ProRule:PRU00388, ECO:0000255|PROSITE-ProRule:PRU10133}; CC -!- PATHWAY: Protein modification; protein ubiquitination. CC {ECO:0000250|UniProtKB:P63146, ECO:0000255|PROSITE-ProRule:PRU00388}. CC -!- SUBUNIT: Interacts with RAD18, UBR2 and WAC. CC {ECO:0000250|UniProtKB:P63146}. CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15946855}. CC Nucleus {ECO:0000269|PubMed:15946855}. Note=In peripheral neurons, CC expressed both at the plasma membrane and in nuclei. CC -!- TISSUE SPECIFICITY: Expressed at high levels in testes, moderate levels CC in muscle, heart, and brain, and low levels in liver and kidney. Upon CC fasting, increased levels were seen in muscle, heart, liver, and CC kidney. {ECO:0000269|PubMed:8048511}. CC -!- INDUCTION: Up-regulated by NGF. {ECO:0000269|PubMed:15946855}. CC -!- SIMILARITY: Belongs to the ubiquitin-conjugating enzyme family. CC {ECO:0000255|PROSITE-ProRule:PRU00388}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M62388; AAA21087.1; -; mRNA. DR EMBL; U04308; AAB60669.1; -; Genomic_DNA. DR EMBL; U04303; AAB60669.1; JOINED; Genomic_DNA. DR EMBL; U04304; AAB60669.1; JOINED; Genomic_DNA. DR EMBL; U04305; AAB60669.1; JOINED; Genomic_DNA. DR EMBL; U04306; AAB60669.1; JOINED; Genomic_DNA. DR EMBL; U04307; AAB60669.1; JOINED; Genomic_DNA. DR EMBL; AF144083; AAD37966.1; -; mRNA. DR EMBL; BC070946; AAH70946.1; -; mRNA. DR PIR; I51913; I51913. DR RefSeq; NP_112400.1; NM_031138.2. DR AlphaFoldDB; P63149; -. DR BMRB; P63149; -. DR SMR; P63149; -. DR BioGRID; 249674; 1. DR FunCoup; P63149; 2706. DR STRING; 10116.ENSRNOP00000016742; -. DR iPTMnet; P63149; -. DR PhosphoSitePlus; P63149; -. DR jPOST; P63149; -. DR PaxDb; 10116-ENSRNOP00000016742; -. DR GeneID; 81816; -. DR KEGG; rno:81816; -. DR UCSC; RGD:708345; rat. DR AGR; RGD:708345; -. DR CTD; 7320; -. DR RGD; 708345; Ube2b. DR eggNOG; KOG0419; Eukaryota. DR HOGENOM; CLU_030988_10_2_1; -. DR InParanoid; P63149; -. DR OrthoDB; 9984419at2759; -. DR PhylomeDB; P63149; -. DR Reactome; R-RNO-110314; Recognition of DNA damage by PCNA-containing replication complex. DR Reactome; R-RNO-8866652; Synthesis of active ubiquitin: roles of E1 and E2 enzymes. DR Reactome; R-RNO-8866654; E3 ubiquitin ligases ubiquitinate target proteins. DR Reactome; R-RNO-983168; Antigen processing: Ubiquitination & Proteasome degradation. DR UniPathway; UPA00143; -. DR PRO; PR:P63149; -. DR Proteomes; UP000002494; Unplaced. DR GO; GO:0000785; C:chromatin; ISO:RGD. DR GO; GO:0005737; C:cytoplasm; ISO:RGD. DR GO; GO:0033503; C:HULC complex; ISS:UniProtKB. DR GO; GO:0005634; C:nucleus; EXP:UniProtKB. DR GO; GO:0005886; C:plasma membrane; EXP:UniProtKB. DR GO; GO:0005657; C:replication fork; ISO:RGD. DR GO; GO:0001741; C:XY body; ISO:RGD. DR GO; GO:0061631; F:ubiquitin conjugating enzyme activity; ISS:UniProtKB. DR GO; GO:0031625; F:ubiquitin protein ligase binding; ISO:RGD. DR GO; GO:0004842; F:ubiquitin-protein transferase activity; ISS:UniProtKB. DR GO; GO:0032869; P:cellular response to insulin stimulus; IEP:RGD. DR GO; GO:0051026; P:chiasma assembly; ISO:RGD. DR GO; GO:0006325; P:chromatin organization; ISO:RGD. DR GO; GO:0006974; P:DNA damage response; ISO:RGD. DR GO; GO:0006301; P:DNA damage tolerance; ISO:RGD. DR GO; GO:0006281; P:DNA repair; ISO:RGD. DR GO; GO:0001701; P:in utero embryonic development; ISO:RGD. DR GO; GO:0045141; P:meiotic telomere clustering; ISO:RGD. DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:RGD. DR GO; GO:1901874; P:negative regulation of post-translational protein modification; ISO:RGD. DR GO; GO:0090263; P:positive regulation of canonical Wnt signaling pathway; ISO:RGD. DR GO; GO:0010845; P:positive regulation of reciprocal meiotic recombination; ISO:RGD. DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; ISO:RGD. DR GO; GO:0051865; P:protein autoubiquitination; ISO:RGD. DR GO; GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB. DR GO; GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB. DR GO; GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB. DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central. DR GO; GO:0050821; P:protein stabilization; ISO:RGD. DR GO; GO:0016567; P:protein ubiquitination; ISO:RGD. DR GO; GO:0001666; P:response to hypoxia; IEP:RGD. DR GO; GO:0009411; P:response to UV; ISO:RGD. DR GO; GO:0009410; P:response to xenobiotic stimulus; ISO:RGD. DR GO; GO:0007288; P:sperm axoneme assembly; ISO:RGD. DR GO; GO:0070193; P:synaptonemal complex organization; ISO:RGD. DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEP:RGD. DR CDD; cd23790; UBCc_UBE2A_2B; 1. DR FunFam; 3.10.110.10:FF:000062; Ubiquitin-conjugating enzyme E2 B; 1. DR Gene3D; 3.10.110.10; Ubiquitin Conjugating Enzyme; 1. DR InterPro; IPR050113; Ub_conjugating_enzyme-E2-like. DR InterPro; IPR000608; UBC. DR InterPro; IPR023313; UBQ-conjugating_AS. DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD. DR PANTHER; PTHR24067; UBIQUITIN-CONJUGATING ENZYME E2; 1. DR Pfam; PF00179; UQ_con; 1. DR SMART; SM00212; UBCc; 1. DR SUPFAM; SSF54495; UBC-like; 1. DR PROSITE; PS00183; UBC_1; 1. DR PROSITE; PS50127; UBC_2; 1. PE 2: Evidence at transcript level; KW Cell membrane; DNA damage; DNA repair; Membrane; Nucleus; KW Reference proteome; Transferase; Ubl conjugation pathway. FT CHAIN 1..152 FT /note="Ubiquitin-conjugating enzyme E2 B" FT /id="PRO_0000082450" FT DOMAIN 4..150 FT /note="UBC core" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388" FT ACT_SITE 88 FT /note="Glycyl thioester intermediate" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00388, FT ECO:0000255|PROSITE-ProRule:PRU10133" SQ SEQUENCE 152 AA; 17312 MW; CFDEEEE7E06840BE CRC64; MSTPARRRLM RDFKRLQEDP PVGVSGAPSE NNIMQWNAVI FGPEGTPFED GTFKLVIEFS EEYPNKPPTV RFLSKMFHPN VYADGSICLD ILQNRWSPTY DVSSILTSIQ SLLDEPNPNS PANSQAAQLY QENKREYEKR VSAIVEQSWN DS //