ID   DAD1_RAT                Reviewed;         113 AA.
AC   P61805; O08552; O70364; P46966; P46968; Q96GB7;
DT   07-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   02-SEP-2026, entry version 133.
DE   RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1 {ECO:0000305};
DE            Short=Oligosaccharyl transferase subunit DAD1;
DE   AltName: Full=Defender against cell death 1;
DE            Short=DAD-1;
GN   Name=Dad1 {ECO:0000312|RGD:621028};
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Lewis;
RA   Giegerich G.;
RT   "Molecular cloning of the murine homologue of DAD-1, an ubiquitously
RT   expressed and highly conserved suppressor of apoptosis.";
RL   Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Pituitary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC       catalyzes the initial transfer of a defined glycan
CC       (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC       pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC       consensus motif in nascent polypeptide chains, the first step in
CC       protein N-glycosylation (By similarity). N-glycosylation occurs
CC       cotranslationally and the complex associates with the Sec61 complex at
CC       the channel-forming translocon complex that mediates protein
CC       translocation across the endoplasmic reticulum (ER). All subunits are
CC       required for a maximal enzyme activity. {ECO:0000250|UniProtKB:E2R4X3,
CC       ECO:0000250|UniProtKB:P61803}.
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC       {ECO:0000250|UniProtKB:P61803}.
CC   -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex (By
CC       similarity). OST exists in two different complex forms which contain
CC       common core subunits RPN1, RPN2, OST48, OST4, DAD1 and TMEM258, either
CC       STT3A or STT3B as catalytic subunits, and form-specific accessory
CC       subunits (By similarity). STT3A complex assembly occurs through the
CC       formation of 3 subcomplexes. Subcomplex 1 contains RPN1 and TMEM258,
CC       subcomplex 2 contains the STT3A-specific subunits STT3A, DC2/OSTC, and
CC       KCP2 as well as the core subunit OST4, and subcomplex 3 contains RPN2,
CC       DAD1, and OST48. The STT3A complex can form stable complexes with the
CC       Sec61 complex or with both the Sec61 and TRAP complexes.
CC       {ECO:0000250|UniProtKB:E2R4X3, ECO:0000250|UniProtKB:P61803}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DAD/OST2 family. {ECO:0000305}.
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DR   EMBL; Y13336; CAA73780.1; -; Genomic_DNA.
DR   EMBL; BC061530; AAH61530.1; -; mRNA.
DR   RefSeq; NP_620265.1; NM_138910.4.
DR   AlphaFoldDB; P61805; -.
DR   SMR; P61805; -.
DR   FunCoup; P61805; 2453.
DR   IntAct; P61805; 2.
DR   MINT; P61805; -.
DR   STRING; 10116.ENSRNOP00000012233; -.
DR   PhosphoSitePlus; P61805; -.
DR   jPOST; P61805; -.
DR   PaxDb; 10116-ENSRNOP00000012233; -.
DR   Ensembl; ENSRNOT00000098684.2; ENSRNOP00000092852.1; ENSRNOG00000009090.7.
DR   Ensembl; ENSRNOT00055020459; ENSRNOP00055016463; ENSRNOG00055012038.
DR   Ensembl; ENSRNOT00060044393; ENSRNOP00060036789; ENSRNOG00060025638.
DR   Ensembl; ENSRNOT00065057504; ENSRNOP00065047345; ENSRNOG00065033446.
DR   GeneID; 192275; -.
DR   KEGG; rno:192275; -.
DR   UCSC; RGD:621028; rat.
DR   AGR; RGD:621028; -.
DR   CTD; 1603; -.
DR   RGD; 621028; Dad1.
DR   eggNOG; KOG1746; Eukaryota.
DR   GeneTree; ENSGT00390000003324; -.
DR   HOGENOM; CLU_111220_2_1_1; -.
DR   InParanoid; P61805; -.
DR   PhylomeDB; P61805; -.
DR   Reactome; R-RNO-9768727; Regulation of CDH1 posttranslational processing and trafficking to plasma membrane.
DR   Reactome; R-RNO-9931295; PD-L1(CD274) glycosylation and translocation to plasma membrane.
DR   UniPathway; UPA00378; -.
DR   PRO; PR:P61805; -.
DR   Proteomes; UP000002494; Chromosome 15.
DR   Bgee; ENSRNOG00000009090; Expressed in pancreas and 20 other cell types or tissues.
DR   ExpressionAtlas; P61805; baseline and differential.
DR   GO; GO:0008250; C:oligosaccharyltransferase complex; ISS:UniProtKB.
DR   GO; GO:0160226; C:oligosaccharyltransferase complex A; ISO:RGD.
DR   GO; GO:0160227; C:oligosaccharyltransferase complex B; ISO:RGD.
DR   GO; GO:0008047; F:enzyme activator activity; ISO:RGD.
DR   GO; GO:0001824; P:blastocyst development; ISO:RGD.
DR   GO; GO:0009101; P:glycoprotein biosynthetic process; ISS:UniProtKB.
DR   GO; GO:0043066; P:negative regulation of apoptotic process; ISO:RGD.
DR   GO; GO:0006487; P:protein N-linked glycosylation; ISO:RGD.
DR   GO; GO:0031647; P:regulation of protein stability; ISO:RGD.
DR   GO; GO:0007584; P:response to nutrient; IEP:RGD.
DR   GO; GO:0009410; P:response to xenobiotic stimulus; IEP:RGD.
DR   InterPro; IPR003038; DAD/Ost2.
DR   PANTHER; PTHR10705; DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1; 1.
DR   PANTHER; PTHR10705:SF0; DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT DAD1; 1.
DR   Pfam; PF02109; DAD; 1.
DR   PIRSF; PIRSF005588; DAD; 1.
PE   3: Inferred from homology;
KW   Acetylation; Apoptosis; Endoplasmic reticulum; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:P61803"
FT   CHAIN           2..113
FT                   /note="Dolichyl-diphosphooligosaccharide--protein
FT                   glycosyltransferase subunit DAD1"
FT                   /id="PRO_0000124014"
FT   TOPO_DOM        2..30
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        31..51
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        52
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        53..73
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        74..92
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        93..113
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         2
FT                   /note="N-acetylserine"
FT                   /evidence="ECO:0000250|UniProtKB:P61803"
SQ   SEQUENCE   113 AA;  12497 MW;  481983CADCEB2345 CRC64;
     MSASVVSVIS RFLEEYLSST PQRLKLLDAY LLYILLTGAL QFGYCLLVGT FPFNSFLSGF
     ISCVGSFILA VCLRIQINPQ NKADFQGISP ERAFADFLFA STILHLVVMN FVG
//