ID   SF3B6_MOUSE             Reviewed;         125 AA.
AC   P59708; Q3U9T9;
DT   06-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT   06-JUN-2003, sequence version 1.
DT   02-SEP-2026, entry version 157.
DE   RecName: Full=Splicing factor 3B subunit 6;
DE   AltName: Full=Pre-mRNA branch site protein p14;
DE   AltName: Full=SF3b 14 kDa subunit;
GN   Name=Sf3b6; Synonyms=Sf3b14;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Bone marrow;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Mammary tumor;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain;
RX   PubMed=16452087; DOI=10.1074/mcp.t500041-mcp200;
RA   Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.;
RT   "Comprehensive identification of phosphorylation sites in postsynaptic
RT   density preparations.";
RL   Mol. Cell. Proteomics 5:914-922(2006).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Brain, Pancreas, Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Component of the 17S U2 SnRNP complex of the spliceosome, a
CC       large ribonucleoprotein complex that removes introns from transcribed
CC       pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early
CC       spliceosome assembly and (2) mediates recognition of the intron branch
CC       site during pre-mRNA splicing by promoting the selection of the pre-
CC       mRNA branch-site adenosine, the nucleophile for the first step of
CC       splicing. Within the 17S U2 SnRNP complex, SF3B6 is part of the SF3B
CC       subcomplex, which is required for 'A' complex assembly formed by the
CC       stable binding of U2 snRNP to the branchpoint sequence in pre-mRNA.
CC       Sequence independent binding of SF3A and SF3B subcomplexes upstream of
CC       the branch site is essential, it may anchor U2 snRNP to the pre-mRNA.
CC       Within the 17S U2 SnRNP complex, SF3B6 directly contacts the pre-mRNA
CC       branch site adenosine for the first catalytic step of splicing. SF3B6
CC       stabilizes the intron branch site-U2 snRNA duplex, thereby promoting-
CC       binding of introns with poor sequence complementarity. Also acts as a
CC       component of the minor spliceosome, which is involved in the splicing
CC       of U12-type introns in pre-mRNAs. {ECO:0000250|UniProtKB:Q9Y3B4}.
CC   -!- SUBUNIT: Component of the 17S U2 SnRNP complex, a ribonucleoprotein
CC       complex that contains small nuclear RNA (snRNA) U2 and a number of
CC       specific proteins. Part of the SF3B subcomplex of the 17S U2 SnRNP
CC       complex. SF3B associates with the splicing subcomplex SF3A and a 12S
CC       RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2
CC       snRNP). Within the SF3B complex interacts directly with SF3B1.
CC       Component of the minor spliceosome, which splices U12-type introns.
CC       {ECO:0000250|UniProtKB:Q9Y3B4}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y3B4}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AK002375; BAB22052.1; -; mRNA.
DR   EMBL; AK003683; BAB22936.1; -; mRNA.
DR   EMBL; AK151648; BAE30577.1; -; mRNA.
DR   EMBL; BC019535; AAH19535.1; -; mRNA.
DR   CCDS; CCDS25791.1; -.
DR   RefSeq; NP_079599.1; NM_025323.2.
DR   AlphaFoldDB; P59708; -.
DR   BMRB; P59708; -.
DR   SMR; P59708; -.
DR   BioGRID; 211182; 10.
DR   FunCoup; P59708; 3960.
DR   IntAct; P59708; 4.
DR   MINT; P59708; -.
DR   STRING; 10090.ENSMUSP00000043662; -.
DR   GlyGen; P59708; 1 site, 1 O-linked glycan (1 site).
DR   iPTMnet; P59708; -.
DR   PhosphoSitePlus; P59708; -.
DR   SwissPalm; P59708; -.
DR   jPOST; P59708; -.
DR   PaxDb; 10090-ENSMUSP00000043662; -.
DR   PeptideAtlas; P59708; -.
DR   ProteomicsDB; 261508; -.
DR   Pumba; P59708; -.
DR   Antibodypedia; 27422; 162 antibodies from 25 providers.
DR   DNASU; 66055; -.
DR   Ensembl; ENSMUST00000046207.9; ENSMUSP00000043662.8; ENSMUSG00000037361.9.
DR   GeneID; 66055; -.
DR   KEGG; mmu:66055; -.
DR   UCSC; uc007myi.1; mouse.
DR   AGR; MGI:1913305; -.
DR   CTD; 51639; -.
DR   MGI; MGI:1913305; Sf3b6.
DR   VEuPathDB; HostDB:ENSMUSG00000037361; -.
DR   eggNOG; KOG0114; Eukaryota.
DR   GeneTree; ENSGT00390000005908; -.
DR   HOGENOM; CLU_012062_25_2_1; -.
DR   InParanoid; P59708; -.
DR   OMA; HQPDKMV; -.
DR   OrthoDB; 275748at2759; -.
DR   PhylomeDB; P59708; -.
DR   BioGRID-ORCS; 66055; 24 hits in 76 CRISPR screens.
DR   PRO; PR:P59708; -.
DR   Proteomes; UP000000589; Chromosome 12.
DR   RNAct; P59708; protein.
DR   Bgee; ENSMUSG00000037361; Expressed in saccule of membranous labyrinth and 257 other cell types or tissues.
DR   ExpressionAtlas; P59708; baseline and differential.
DR   GO; GO:0005654; C:nucleoplasm; ISO:GO_Central.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0071011; C:precatalytic spliceosome; ISO:GO_Central.
DR   GO; GO:0005681; C:spliceosomal complex; ISO:GO_Central.
DR   GO; GO:0034693; C:U11/U12 snRNP; ISO:GO_Central.
DR   GO; GO:0071018; C:U12-type catalytic step 2 spliceosome; ISO:GO_Central.
DR   GO; GO:0071016; C:U12-type precatalytic spliceosome; ISO:GO_Central.
DR   GO; GO:0005689; C:U12-type spliceosomal complex; ISO:GO_Central.
DR   GO; GO:0071006; C:U2-type catalytic step 1 spliceosome; ISO:GO_Central.
DR   GO; GO:0005684; C:U2-type spliceosomal complex; ISO:GO_Central.
DR   GO; GO:0003729; F:mRNA binding; ISS:UniProtKB.
DR   GO; GO:0001825; P:blastocyst formation; IMP:MGI.
DR   GO; GO:0000398; P:mRNA splicing, via spliceosome; IMP:MGI.
DR   CDD; cd12241; RRM_SF3B14; 1.
DR   FunFam; 3.30.70.330:FF:000106; Splicing factor 3b, subunit 6; 1.
DR   Gene3D; 3.30.70.330; -; 1.
DR   InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR   InterPro; IPR035979; RBD_domain_sf.
DR   InterPro; IPR000504; RRM_dom.
DR   InterPro; IPR050374; RRT5_SRSF_SR.
DR   InterPro; IPR034150; SF3B6_RRM.
DR   PANTHER; PTHR23003; RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN; 1.
DR   Pfam; PF00076; RRM_1; 1.
DR   SMART; SM00360; RRM; 1.
DR   SUPFAM; SSF54928; RNA-binding domain, RBD; 1.
DR   PROSITE; PS50102; RRM; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Isopeptide bond; mRNA processing; mRNA splicing; Nucleus;
KW   Reference proteome; RNA-binding; Spliceosome; Ubl conjugation.
FT   CHAIN           1..125
FT                   /note="Splicing factor 3B subunit 6"
FT                   /id="PRO_0000081726"
FT   DOMAIN          19..94
FT                   /note="RRM"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT   REGION          16..29
FT                   /note="Interaction with pre-mRNA branch site"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         29
FT                   /note="N6-acetyllysine; alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y3B4"
FT   MOD_RES         41
FT                   /note="N6-acetyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y3B4"
FT   CROSSLNK        29
FT                   /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT                   G-Cter in SUMO2); alternate"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y3B4"
SQ   SEQUENCE   125 AA;  14585 MW;  A1439DACB78A3208 CRC64;
     MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA
     YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN
     TDPPK
//