ID   GUN_RALN1               Reviewed;         424 AA.
AC   P58599;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2002, sequence version 1.
DT   04-NOV-2026, entry version 124.
DE   RecName: Full=Endoglucanase;
DE            EC=3.2.1.4;
DE   AltName: Full=Cellulase;
DE   AltName: Full=Endo-1,4-beta-glucanase;
DE   Flags: Precursor;
GN   Name=egl; OrderedLocusNames=RSp0162; ORFNames=RS05516;
OS   Ralstonia nicotianae (strain ATCC BAA-1114 / GMI1000) (Ralstonia
OS   solanacearum).
OG   Plasmid megaplasmid Rsp.
OC   Bacteria; Pseudomonadati; Pseudomonadota; Betaproteobacteria;
OC   Burkholderiales; Burkholderiaceae; Ralstonia;
OC   Ralstonia solanacearum species complex.
OX   NCBI_TaxID=267608;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1114 / GMI1000;
RX   PubMed=11823852; DOI=10.1038/415497a;
RA   Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA   Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA   Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA   Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA   Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT   "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL   Nature 415:497-502(2002).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 5 (cellulase A) family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAD17313.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AL646053; CAD17313.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; P58599; -.
DR   SMR; P58599; -.
DR   STRING; 267608.RSp0162; -.
DR   EnsemblBacteria; CAD17313; CAD17313; RSp0162.
DR   KEGG; rso:RSp0162; -.
DR   eggNOG; COG2730; Bacteria.
DR   HOGENOM; CLU_029718_0_0_4; -.
DR   OMA; AACMDGK; -.
DR   Proteomes; UP000001436; Plasmid pGMI1000MP.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009251; P:glucan catabolic process; IEA:TreeGrafter.
DR   FunFam; 3.20.20.80:FF:000124; Exported cellulase; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR017853; GH_hydrolase_sf.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR018087; Glyco_hydro_5_CS.
DR   PANTHER; PTHR34142; ENDO-BETA-1,4-GLUCANASE A; 1.
DR   PANTHER; PTHR34142:SF1; GLYCOSIDE HYDROLASE FAMILY 5 DOMAIN-CONTAINING PROTEIN; 1.
DR   Pfam; PF00150; Cellulase; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism; Cell membrane; Cellulose degradation; Glycosidase;
KW   Hydrolase; Lipoprotein; Membrane; Palmitate; Plasmid;
KW   Polysaccharide degradation; Reference proteome; Signal; Zymogen.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   PROPEP          20..43
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000007867"
FT   CHAIN           44..424
FT                   /note="Endoglucanase"
FT                   /id="PRO_0000007868"
FT   ACT_SITE        247
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        359
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   424 AA;  45081 MW;  5BF0D161AB7C87EF CRC64;
     MHRCMPLVAA SMAALMLAGC GGGDGDTTLS TAAATDTTTL KTAATTSISP LWLTIAKDSA
     AFTVSGTRTV RYGAGSAWVA KSMSGTGQCT AAFFGKDPAA GVAKVCQVAQ GTGTLLWRGV
     SLAGAEFGEG SLPGTYGSNY IYPSADSATY YKNKGMNLVR LPFRWERLQP TLNQALDANE
     LSRLTGFVNA VTAAGQTVLL DPHNYARYYG NVIGSSAVPN SAYADFWRRV ATQFKGNARV
     IFGLMNEPNS MPTEQWLSGA NAALAAIRSA NASNVVFVPG NAWTGAHSWN QNWYGTPNGT
     VMKGINDPGR NLVFEVHQYL DGDSSGQSAS CVSATIGAER LQDFTNWLRS NGYRGFLGEF
     GAASNDTCNQ AVANMLTFVK NNADVWTGWA WWAGGPWWGG YMYSIEPSNG VDKPQMSVLA
     PYLK
//