ID   NUSG_BUCAI              Reviewed;         181 AA.
AC   P57151;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   04-NOV-2026, entry version 120.
DE   RecName: Full=Transcription termination/antitermination protein NusG {ECO:0000255|HAMAP-Rule:MF_00948};
GN   Name=nusG {ECO:0000255|HAMAP-Rule:MF_00948}; OrderedLocusNames=BU039;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Participates in transcription elongation, termination and
CC       antitermination. In the absence of Rho, increases the rate of
CC       transcription elongation by the RNA polymerase (RNAP), probably by
CC       partially suppressing pausing. In the presence of Rho, modulates most
CC       Rho-dependent termination events by interacting with the RNAP to render
CC       the complex more susceptible to the termination activity of Rho. May be
CC       required to overcome a kinetic limitation of Rho to function at certain
CC       terminators. Also involved in ribosomal RNA transcriptional
CC       antitermination. {ECO:0000255|HAMAP-Rule:MF_00948}.
CC   -!- SUBUNIT: Monomer. Interacts with the transcription termination factor
CC       Rho and with RNA polymerase. {ECO:0000255|HAMAP-Rule:MF_00948}.
CC   -!- SIMILARITY: Belongs to the NusG family. {ECO:0000255|HAMAP-
CC       Rule:MF_00948}.
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DR   EMBL; BA000003; BAB12766.1; -; Genomic_DNA.
DR   RefSeq; WP_009874000.1; NC_002528.1.
DR   AlphaFoldDB; P57151; -.
DR   SMR; P57151; -.
DR   STRING; 563178.BUAP5A_038; -.
DR   EnsemblBacteria; BAB12766; BAB12766; BAB12766.
DR   KEGG; buc:BU039; -.
DR   PATRIC; fig|107806.10.peg.52; -.
DR   eggNOG; COG0250; Bacteria.
DR   HOGENOM; CLU_067287_1_0_6; -.
DR   OMA; AAGEWYV; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR   GO; GO:0006354; P:DNA-templated transcription elongation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription termination; IEA:UniProtKB-UniRule.
DR   GO; GO:0032784; P:regulation of DNA-templated transcription elongation; IEA:InterPro.
DR   GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR   CDD; cd06091; KOW_NusG; 1.
DR   CDD; cd09891; NGN_Bact_1; 1.
DR   FunFam; 2.30.30.30:FF:000002; Transcription termination/antitermination factor NusG; 1.
DR   FunFam; 3.30.70.940:FF:000001; Transcription termination/antitermination protein NusG; 1.
DR   Gene3D; 2.30.30.30; -; 1.
DR   Gene3D; 3.30.70.940; NusG, N-terminal domain; 1.
DR   HAMAP; MF_00948; NusG; 1.
DR   InterPro; IPR005824; KOW.
DR   InterPro; IPR047050; NGN.
DR   InterPro; IPR006645; NGN-like_dom.
DR   InterPro; IPR036735; NGN_dom_sf.
DR   InterPro; IPR043425; NusG-like.
DR   InterPro; IPR014722; Rib_uL2_dom2.
DR   InterPro; IPR001062; Transcrpt_antiterm_NusG.
DR   InterPro; IPR015869; Transcrpt_antiterm_NusG_bac_CS.
DR   InterPro; IPR008991; Translation_prot_SH3-like_sf.
DR   NCBIfam; TIGR00922; nusG; 1.
DR   PANTHER; PTHR30265; RHO-INTERACTING TRANSCRIPTION TERMINATION FACTOR NUSG; 1.
DR   PANTHER; PTHR30265:SF2; TRANSCRIPTION TERMINATION_ANTITERMINATION PROTEIN NUSG; 1.
DR   Pfam; PF00467; KOW; 1.
DR   Pfam; PF02357; NusG; 1.
DR   PRINTS; PR00338; NUSGTNSCPFCT.
DR   SMART; SM00739; KOW; 1.
DR   SMART; SM00738; NGN; 1.
DR   SUPFAM; SSF82679; N-utilization substance G protein NusG, N-terminal domain; 1.
DR   SUPFAM; SSF50104; Translation proteins SH3-like domain; 1.
DR   PROSITE; PS01014; NUSG; 1.
PE   3: Inferred from homology;
KW   Reference proteome; Transcription; Transcription antitermination;
KW   Transcription regulation; Transcription termination.
FT   CHAIN           1..181
FT                   /note="Transcription termination/antitermination protein
FT                   NusG"
FT                   /id="PRO_0000113918"
FT   DOMAIN          130..161
FT                   /note="KOW"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00948"
SQ   SEQUENCE   181 AA;  20960 MW;  6CC58AA1DB43F411 CRC64;
     MHESQKKRWY VLQAFSGFES RVAQSIREHV KLNQMEDLFG EVMVPSQEVV EIRGGQRRKS
     EYKFFPGYVL IQMKMTDSTW HLIRNVPKVL GFIGGKSDKP SPISDKEVEI IINRLRKIGN
     KPRPKTLFEP GEMIRVNDGP FADFNGVVEE VDYEKSRLKV SVSIFGRSTP VELDFRQVEK
     N
//