ID FER1_SULTO Reviewed; 104 AA. AC P55907; O32423; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 23-JAN-2007, sequence version 3. DT 02-SEP-2026, entry version 152. DE RecName: Full=Zinc-containing ferredoxin-1; GN Name=zfx1; Synonyms=zfx; OrderedLocusNames=STK_01630; OS Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OS (Sulfolobus tokodaii). OC Archaea; Thermoproteota; Thermoprotei; Sulfolobales; Sulfolobaceae; OC Sulfurisphaera. OX NCBI_TaxID=273063; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7; RX PubMed=8753789; DOI=10.1006/bbrc.1996.1200; RA Wakagi T., Fujii T., Oshima T.; RT "Molecular cloning, sequencing, and heterologous expression of a novel RT zinc-containing ferredoxin gene from a thermoacidophilic Archaeon RT Sulfolobus sp. strain 7."; RL Biochem. Biophys. Res. Commun. 225:489-493(1996). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7; RX PubMed=11572479; DOI=10.1093/dnares/8.4.123; RA Kawarabayasi Y., Hino Y., Horikawa H., Jin-no K., Takahashi M., Sekine M., RA Baba S., Ankai A., Kosugi H., Hosoyama A., Fukui S., Nagai Y., RA Nishijima K., Otsuka R., Nakazawa H., Takamiya M., Kato Y., Yoshizawa T., RA Tanaka T., Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., RA Masuda S., Yanagii M., Nishimura M., Yamagishi A., Oshima T., Kikuchi H.; RT "Complete genome sequence of an aerobic thermoacidophilic Crenarchaeon, RT Sulfolobus tokodaii strain7."; RL DNA Res. 8:123-140(2001). RN [3] RP PROTEIN SEQUENCE OF 2-31, AND METHYLATION AT LYS-30. RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7; RX PubMed=7826373; DOI=10.1006/bbrc.1995.1080; RA Iwasaki T., Fujii T., Wakagi T., Oshima T.; RT "Alternative form of the dicluster ferredoxin from the thermoacidophilic RT archaeon, Sulfolobus sp. strain 7."; RL Biochem. Biophys. Res. Commun. 206:563-569(1995). RN [4] RP CHARACTERIZATION. RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7; RX PubMed=7961925; DOI=10.1016/s0021-9258(18)43899-9; RA Iwasaki T., Wakagi T., Isogai Y., Tanaka K., Iizuka T., Oshima T.; RT "Functional and evolutionary implications of a [3Fe-4S] cluster of the RT dicluster-type ferredoxin from the thermoacidophilic archaeon, Sulfolobus RT sp. strain 7."; RL J. Biol. Chem. 269:29444-29450(1994). RN [5] RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). RC STRAIN=DSM 16993 / JCM 10545 / NBRC 100140 / 7; RX PubMed=9063899; DOI=10.1021/bi961966j; RA Fujii T., Hata Y., Oozeki M., Moriyama H., Wakagi T., Tanaka N., Oshima T.; RT "The crystal structure of zinc-containing ferredoxin from the RT thermoacidophilic archaeon Sulfolobus sp. strain 7."; RL Biochemistry 36:1505-1513(1997). CC -!- FUNCTION: Ferredoxins are iron-sulfur proteins that transfer electrons CC in a wide variety of metabolic reactions. CC -!- COFACTOR: CC Name=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137; CC Note=Binds 1 [3Fe-4S] cluster.; CC -!- COFACTOR: CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; CC Note=Binds 1 [4Fe-4S] cluster.; CC -!- COFACTOR: CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; CC Note=Binds 1 zinc ion.; CC -!- BIOPHYSICOCHEMICAL PROPERTIES: CC Redox potential: CC E(0) is -280 mV for the 3Fe-4S and -530 mV for the 4Fe-4S clusters.; CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D78179; BAA22587.1; -; Genomic_DNA. DR EMBL; BA000023; BAB65120.1; -; Genomic_DNA. DR PIR; JC4907; JC4907. DR RefSeq; WP_010978102.1; NC_003106.2. DR PDB; 1XER; X-ray; 2.00 A; A=2-104. DR AlphaFoldDB; P55907; -. DR SMR; P55907; -. DR STRING; 273063.STK_01630; -. DR iPTMnet; P55907; -. DR GeneID; 54121801; -. DR GeneID; 92354065; -. DR KEGG; sto:STK_01630; -. DR PATRIC; fig|273063.9.peg.200; -. DR eggNOG; arCOG04548; Archaea. DR OrthoDB; 23833at2157; -. DR EvolutionaryTrace; P55907; -. DR Proteomes; UP000001015; Chromosome. DR GO; GO:0009055; F:electron transfer activity; IEA:InterPro. DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro. DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-ARBA. DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro. DR Gene3D; 3.30.70.20; -; 1. DR InterPro; IPR017896; 4Fe4S_Fe-S-bd. DR InterPro; IPR017900; 4Fe4S_Fe_S_CS. DR InterPro; IPR009157; Fd_Zn-bd. DR InterPro; IPR050572; Fe-S_Ferredoxin. DR PANTHER; PTHR43687; ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT; 1. DR PANTHER; PTHR43687:SF6; L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT; 1. DR Pfam; PF13237; Fer4_10; 1. DR PIRSF; PIRSF000068; Zn_Fdx_Sulfol; 1. DR SUPFAM; SSF54862; 4Fe-4S ferredoxins; 1. DR PROSITE; PS00198; 4FE4S_FER_1; 1. DR PROSITE; PS51379; 4FE4S_FER_2; 2. PE 1: Evidence at protein level; KW 3D-structure; 3Fe-4S; 4Fe-4S; Direct protein sequencing; KW Electron transport; Iron; Iron-sulfur; Metal-binding; Methylation; KW Reference proteome; Repeat; Transport; Zinc. FT INIT_MET 1 FT /note="Removed" FT /evidence="ECO:0000269|PubMed:7826373" FT CHAIN 2..104 FT /note="Zinc-containing ferredoxin-1" FT /id="PRO_0000159180" FT DOMAIN 38..66 FT /note="4Fe-4S ferredoxin-type 1" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711" FT DOMAIN 75..104 FT /note="4Fe-4S ferredoxin-type 2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711" FT REGION 2..37 FT /note="N-terminal extension" FT BINDING 17 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT BINDING 20 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT BINDING 35 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT BINDING 46 FT /ligand="[3Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:21137" FT BINDING 52 FT /ligand="[3Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:21137" FT BINDING 56 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT BINDING 77 FT /ligand="Zn(2+)" FT /ligand_id="ChEBI:CHEBI:29105" FT BINDING 84 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT BINDING 87 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT BINDING 90 FT /ligand="[4Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:49883" FT BINDING 94 FT /ligand="[3Fe-4S] cluster" FT /ligand_id="ChEBI:CHEBI:21137" FT MOD_RES 30 FT /note="N6-methyllysine" FT /evidence="ECO:0000303|PubMed:7826373" FT CONFLICT 26 FT /note="V -> L (in Ref. 3; AA sequence)" FT /evidence="ECO:0000305" FT CONFLICT 54 FT /note="N -> T (in Ref. 1; BAA22587)" FT /evidence="ECO:0000305" FT HELIX 7..10 FT /evidence="ECO:0007829|PDB:1XER" FT STRAND 13..17 FT /evidence="ECO:0007829|PDB:1XER" FT STRAND 20..24 FT /evidence="ECO:0007829|PDB:1XER" FT TURN 28..30 FT /evidence="ECO:0007829|PDB:1XER" FT STRAND 37..42 FT /evidence="ECO:0007829|PDB:1XER" FT TURN 43..45 FT /evidence="ECO:0007829|PDB:1XER" FT HELIX 51..55 FT /evidence="ECO:0007829|PDB:1XER" FT STRAND 62..65 FT /evidence="ECO:0007829|PDB:1XER" FT STRAND 73..77 FT /evidence="ECO:0007829|PDB:1XER" FT HELIX 81..83 FT /evidence="ECO:0007829|PDB:1XER" FT HELIX 89..93 FT /evidence="ECO:0007829|PDB:1XER" FT STRAND 99..101 FT /evidence="ECO:0007829|PDB:1XER" SQ SEQUENCE 104 AA; 11134 MW; 04A9E52F5EDA4F4A CRC64; MGIDPNYRTN RQVVGEHSGH KVYGPVEPPK VLGIHGTIVG VDFDLCIADG SCINACPVNV FQWYDTPGHP ASEKKADPVN EQACIFCMAC VNVCPVAAID VKPP //