ID   SIX1_BUTOC              Reviewed;          65 AA.
AC   P55902;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   02-SEP-2026, entry version 97.
DE   RecName: Full=Alpha-insect toxin BotIT1;
OS   Buthus occitanus tunetanus (Common European scorpion) (Buthus tunetanus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Buthida; Buthoidea; Buthidae; Buthus.
OX   NCBI_TaxID=6871;
RN   [1]
RP   PROTEIN SEQUENCE, TOXIC DOSE, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=9080593; DOI=10.1016/s0041-0101(96)00173-0;
RA   Borchani L., Stankiewicz M., Kopeyan C., Mansuelle P., Kharrat R.,
RA   Cestele S., Karoui H., Rochat H., Pelhate M., el Ayeb M.;
RT   "Purification, structure and activity of three insect toxins from Buthus
RT   occitanus tunetanus venom.";
RL   Toxicon 35:365-382(1997).
CC   -!- FUNCTION: Alpha toxins bind voltage-independently at site-3 of sodium
CC       channels (Nav) and inhibit the inactivation of the activated channels,
CC       thereby blocking neuronal transmission. This contractive toxin is
CC       highly toxic to insects and barely toxic to mammals.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: Has the structural arrangement of an alpha-helix connected to
CC       antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).
CC       {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7334.93; Mass_error=0.23; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:9080593};
CC   -!- TOXIC DOSE: LD(50) is 50 ug/kg by intracerebroventricular injection
CC       into mice and 600 ng/g in Blattella germanica.
CC       {ECO:0000269|PubMed:9080593}.
CC   -!- SIMILARITY: Belongs to the long (4 C-C) scorpion toxin superfamily.
CC       Sodium channel inhibitor family. Alpha subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P55902; -.
DR   SMR; P55902; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019871; F:sodium channel inhibitor activity; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:InterPro.
DR   CDD; cd23106; neurotoxins_LC_scorpion; 1.
DR   FunFam; 3.30.30.10:FF:000002; Alpha-like toxin BmK-M1; 1.
DR   Gene3D; 3.30.30.10; Knottin, scorpion toxin-like; 1.
DR   InterPro; IPR003614; Knottins.
DR   InterPro; IPR044062; LCN-type_CS_alpha_beta_dom.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   InterPro; IPR018218; Scorpion_toxinL.
DR   InterPro; IPR002061; Scorpion_toxinL/defensin.
DR   Pfam; PF00537; Toxin_3; 1.
DR   PRINTS; PR00285; SCORPNTOXIN.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; Scorpion toxin-like; 1.
DR   PROSITE; PS51863; LCN_CSAB; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Neurotoxin; Secreted; Toxin; Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..65
FT                   /note="Alpha-insect toxin BotIT1"
FT                   /id="PRO_0000066713"
FT   DOMAIN          2..64
FT                   /note="LCN-type CS-alpha/beta"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        12..63
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        16..36
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        22..46
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
FT   DISULFID        26..48
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01210"
SQ   SEQUENCE   65 AA;  7343 MW;  93A1371D877D7123 CRC64;
     VRDAYIAQNY NCVYFCMKDD YCNDLCTKNG ASSGYCQWAG KYGNACWCYA LPDNVPIRIP
     GKCHS
//