ID   TRAI_SINFN              Reviewed;         208 AA.
AC   P55394;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   02-SEP-2026, entry version 130.
DE   RecName: Full=Probable acyl-homoserine-lactone synthase;
DE            EC=2.3.1.184;
DE   AltName: Full=Autoinducer synthesis protein TraI;
GN   Name=traI; OrderedLocusNames=NGR_a04220; ORFNames=y4cL;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
OC   Hyphomicrobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: Required for the synthesis of OHHL (N-(3-oxooctanoyl)-L-
CC       homoserine lactone), an autoinducer molecule which binds to TraR and
CC       thus acts in the control of conjugal transfer. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a fatty acyl-[ACP] + S-adenosyl-L-methionine = an N-acyl-L-
CC         homoserine lactone + S-methyl-5'-thioadenosine + holo-[ACP] + H(+);
CC         Xref=Rhea:RHEA:10096, Rhea:RHEA-COMP:9685, Rhea:RHEA-COMP:14125,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17509, ChEBI:CHEBI:55474,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:64479, ChEBI:CHEBI:138651;
CC         EC=2.3.1.184;
CC   -!- SIMILARITY: Belongs to the autoinducer synthase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00533}.
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DR   EMBL; U00090; AAB92427.1; -; Genomic_DNA.
DR   RefSeq; WP_010875432.1; NC_000914.2.
DR   AlphaFoldDB; P55394; -.
DR   SMR; P55394; -.
DR   KEGG; rhi:NGR_a04220; -.
DR   PATRIC; fig|394.7.peg.444; -.
DR   eggNOG; COG3916; Bacteria.
DR   HOGENOM; CLU_085711_4_0_5; -.
DR   OrthoDB; 6169313at2; -.
DR   Proteomes; UP000001054; Plasmid pNGR234a.
DR   GO; GO:0061579; F:N-acyl homoserine lactone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0007165; P:signal transduction; IEA:TreeGrafter.
DR   Gene3D; 3.40.630.30; -; 1.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR018311; Autoind_synth_CS.
DR   InterPro; IPR001690; Autoind_synthase.
DR   NCBIfam; NF010408; PRK13834.1; 1.
DR   PANTHER; PTHR39322; ACYL-HOMOSERINE-LACTONE SYNTHASE; 1.
DR   PANTHER; PTHR39322:SF1; ISOVALERYL-HOMOSERINE LACTONE SYNTHASE; 1.
DR   Pfam; PF00765; Autoind_synth; 1.
DR   PRINTS; PR01549; AUTOINDCRSYN.
DR   SUPFAM; SSF55729; Acyl-CoA N-acyltransferases (Nat); 1.
DR   PROSITE; PS00949; AUTOINDUCER_SYNTH_1; 1.
DR   PROSITE; PS51187; AUTOINDUCER_SYNTH_2; 1.
PE   3: Inferred from homology;
KW   Autoinducer synthesis; Conjugation; Plasmid; Quorum sensing;
KW   Reference proteome; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..208
FT                   /note="Probable acyl-homoserine-lactone synthase"
FT                   /id="PRO_0000210900"
SQ   SEQUENCE   208 AA;  22826 MW;  DF2F053C7A20D28E CRC64;
     MQILAISKPR NIEEAQLLRS HHELRARVFS DRLGWEVNVV GGCESDTFDD LQPTYILAVS
     SNDRVVGCAR LLPALGPTMV ANVFPSLLSA GHLNAHSSMV ESSRFCVDTF LAESRGDGSI
     HEATLTMFAG IIEWSVANRY TEIVTVTDLR FERILARVGW PLQRIGEPRP IGATVAVAGT
     LPAKADTFMR LRPANYRSQI ISTFGQSA
//