ID   BSC4_YEAST              Reviewed;         131 AA.
AC   P53841; B2Y304; B2Y305; B2Y306; B2Y308; D6W0S5;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   10-JUN-2026, entry version 120.
DE   RecName: Full=Bypass of stop codon protein 4;
GN   Name=BSC4; OrderedLocusNames=YNL269W; ORFNames=N0670;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS VAL-3; GLN-6; THR-9;
RP   LEU-16; GLN-19; SER-22; GLY-32; ASN-42 AND HIS-92.
RC   STRAIN=AS2.101, AS2.1406, AS2.148, AS2.179, AS2.2, AS2.2079, AS2.2080,
RC   AS2.3, AS2.7, AS2.724, AS2.771, AS2.820, AS2.93, and XH1549;
RX   PubMed=18493065; DOI=10.1534/genetics.107.084491;
RA   Cai J., Zhao R., Jiang H., Wang W.;
RT   "De novo origination of a new protein-coding gene in Saccharomyces
RT   cerevisiae.";
RL   Genetics 179:487-496(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=8740425;
RX   DOI=10.1002/(sici)1097-0061(199604)12:5%3c505::aid-yea932%3e3.0.co;2-f;
RA   Sen-Gupta M., Lyck R., Fleig U., Niedenthal R.K., Hegemann J.H.;
RT   "The sequence of a 24,152 bp segment from the left arm of chromosome XIV
RT   from Saccharomyces cerevisiae between the BNI1 and the POL2 genes.";
RL   Yeast 12:505-514(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873; DOI=10.1038/387s093;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   GENE NAME.
RX   PubMed=12711673; DOI=10.1093/nar/gkg330;
RA   Namy O., Duchateau-Nguyen G., Hatin I., Hermann-Le Denmat S., Termier M.,
RA   Rousset J.-P.;
RT   "Identification of stop codon readthrough genes in Saccharomyces
RT   cerevisiae.";
RL   Nucleic Acids Res. 31:2289-2296(2003).
CC   -!- MISCELLANEOUS: Open reading frame that exhibits genomic organization
CC       compatible with a translational readthrough-dependent mode of
CC       expression. The sequence can give a readthrough frequency of 9% when
CC       cloned in a plasmid with reporter genes. When the initial stop codon is
CC       bypassed, translation will go on to the second stop codon 321 bp
CC       downstream.
CC   -!- MISCELLANEOUS: This protein has no orthologs in any species other than
CC       the S.cerevisiae lineage. It may be the result of de novo origination
CC       of a protein-coding gene.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; EU375912; ACB40933.1; -; Genomic_DNA.
DR   EMBL; EU375913; ACB40934.1; -; Genomic_DNA.
DR   EMBL; EU375914; ACB40935.1; -; Genomic_DNA.
DR   EMBL; EU375915; ACB40936.1; -; Genomic_DNA.
DR   EMBL; EU375916; ACB40937.1; -; Genomic_DNA.
DR   EMBL; EU375917; ACB40938.1; -; Genomic_DNA.
DR   EMBL; EU375918; ACB40939.1; -; Genomic_DNA.
DR   EMBL; EU375919; ACB40940.1; -; Genomic_DNA.
DR   EMBL; EU375920; ACB40941.1; -; Genomic_DNA.
DR   EMBL; EU375921; ACB40942.1; -; Genomic_DNA.
DR   EMBL; EU375922; ACB40943.1; -; Genomic_DNA.
DR   EMBL; EU375923; ACB40944.1; -; Genomic_DNA.
DR   EMBL; EU375924; ACB40945.1; -; Genomic_DNA.
DR   EMBL; EU375925; ACB40946.1; -; Genomic_DNA.
DR   EMBL; X92494; CAA63229.1; -; Genomic_DNA.
DR   EMBL; Z71545; CAA96176.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10291.1; -; Genomic_DNA.
DR   PIR; S60913; S60913.
DR   RefSeq; NP_014130.1; NM_001183107.1.
DR   AlphaFoldDB; P53841; -.
DR   SMR; P53841; -.
DR   BioGRID; 35571; 140.
DR   FunCoup; P53841; 21.
DR   STRING; 4932.YNL269W; -.
DR   PaxDb; 4932-YNL269W; -.
DR   EnsemblFungi; YNL269W_mRNA; YNL269W; YNL269W.
DR   GeneID; 855452; -.
DR   KEGG; sce:YNL269W; -.
DR   AGR; SGD:S000005213; -.
DR   SGD; S000005213; BSC4.
DR   VEuPathDB; FungiDB:YNL269W; -.
DR   HOGENOM; CLU_2039888_0_0_1; -.
DR   InParanoid; P53841; -.
DR   BioCyc; YEAST:G3O-33263-MONOMER; -.
DR   PRO; PR:P53841; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P53841; protein.
PE   4: Predicted;
KW   Reference proteome.
FT   CHAIN           1..131
FT                   /note="Bypass of stop codon protein 4"
FT                   /id="PRO_0000064994"
FT   VARIANT         3
FT                   /note="I -> V (in strain: XH1549)"
FT                   /evidence="ECO:0000269|PubMed:18493065"
FT   VARIANT         6
FT                   /note="R -> Q (in strain: AS2.101, AS2.1406, AS2.179,
FT                   AS2.7, AS2.724, AS2.771, AS2.820 and AS2.93)"
FT                   /evidence="ECO:0000269|PubMed:18493065"
FT   VARIANT         9..10
FT                   /note="NK -> T (in strain: XH1549)"
FT   VARIANT         16
FT                   /note="I -> L (in strain: XH1549)"
FT                   /evidence="ECO:0000269|PubMed:18493065"
FT   VARIANT         19
FT                   /note="K -> Q (in strain: XH1549)"
FT                   /evidence="ECO:0000269|PubMed:18493065"
FT   VARIANT         22
FT                   /note="T -> S (in strain: XH1549)"
FT                   /evidence="ECO:0000269|PubMed:18493065"
FT   VARIANT         32
FT                   /note="V -> G (in strain: XH1549)"
FT                   /evidence="ECO:0000269|PubMed:18493065"
FT   VARIANT         42
FT                   /note="S -> N (in strain: AS2.101, AS2.1406, AS2.179,
FT                   AS2.7, AS2.724, AS2.771, AS2.820, AS2.93 and XH1549)"
FT                   /evidence="ECO:0000269|PubMed:18493065"
FT   VARIANT         92
FT                   /note="R -> H (in strain: AS2.724 and AS2.820)"
FT                   /evidence="ECO:0000269|PubMed:18493065"
SQ   SEQUENCE   131 AA;  15314 MW;  F9B0C290CB317BBC CRC64;
     MSIVLRKSNK KNKNCITSKF YTIHIIKIST PVFRAPIAIG ESPYVEWSCL QVVFRKDMVT
     KKTTFAQLIT RLNHFLCQAL KRRDSKTYIL CRTAVFGAMT PFSPRKSHIN NKLPMQPRKK
     KIVIIYVVRF H
//