ID   WSC2_YEAST              Reviewed;         503 AA.
AC   P53832; D6W0R1;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   10-JUN-2026, entry version 162.
DE   RecName: Full=Cell wall integrity and stress response component 2;
DE   Flags: Precursor;
GN   Name=WSC2; OrderedLocusNames=YNL283C; ORFNames=N0583;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169873; DOI=10.1038/387s093;
RA   Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA   Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA   Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA   Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA   Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA   Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA   Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA   Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA   Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA   Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA   Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA   Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA   Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA   Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA   Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA   Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA   Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT   evolutionary implications.";
RL   Nature 387:93-98(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-455 AND SER-458, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ADR376;
RX   PubMed=17330950; DOI=10.1021/pr060559j;
RA   Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA   Elias J.E., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of alpha-factor-arrested
RT   Saccharomyces cerevisiae.";
RL   J. Proteome Res. 6:1190-1197(2007).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA   Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT   "A multidimensional chromatography technology for in-depth phosphoproteome
RT   analysis.";
RL   Mol. Cell. Proteomics 7:1389-1396(2008).
RN   [5]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS].
RX   PubMed=19756047; DOI=10.1038/msb.2009.64;
RA   Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.;
RT   "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals
RT   new roles for protein glycosylation in eukaryotes.";
RL   Mol. Syst. Biol. 5:308-308(2009).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-402; SER-455 AND SER-458, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein.
CC   -!- PTM: N-glycosylated. {ECO:0000269|PubMed:19756047}.
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DR   EMBL; Z71559; CAA96195.1; -; Genomic_DNA.
DR   EMBL; BK006947; DAA10277.1; -; Genomic_DNA.
DR   PIR; S63257; S63257.
DR   RefSeq; NP_014116.1; NM_001183121.1.
DR   AlphaFoldDB; P53832; -.
DR   SMR; P53832; -.
DR   BioGRID; 35558; 122.
DR   FunCoup; P53832; 206.
DR   IntAct; P53832; 18.
DR   MINT; P53832; -.
DR   STRING; 4932.YNL283C; -.
DR   iPTMnet; P53832; -.
DR   PaxDb; 4932-YNL283C; -.
DR   PeptideAtlas; P53832; -.
DR   EnsemblFungi; YNL283C_mRNA; YNL283C; YNL283C.
DR   GeneID; 855438; -.
DR   KEGG; sce:YNL283C; -.
DR   AGR; SGD:S000005227; -.
DR   SGD; S000005227; WSC2.
DR   VEuPathDB; FungiDB:YNL283C; -.
DR   eggNOG; KOG4157; Eukaryota.
DR   HOGENOM; CLU_024893_1_0_1; -.
DR   InParanoid; P53832; -.
DR   OMA; DAHNISK; -.
DR   OrthoDB; 2019572at2759; -.
DR   BioCyc; YEAST:G3O-33274-MONOMER; -.
DR   BioGRID-ORCS; 855438; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P53832; -.
DR   Proteomes; UP000002311; Chromosome XIV.
DR   RNAct; P53832; protein.
DR   GO; GO:0005933; C:cellular bud; IDA:SGD.
DR   GO; GO:0005935; C:cellular bud neck; IDA:SGD.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR   GO; GO:0043332; C:mating projection tip; HDA:SGD.
DR   GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR   GO; GO:0030427; C:site of polarized growth; IDA:SGD.
DR   GO; GO:0004888; F:transmembrane signaling receptor activity; IMP:SGD.
DR   GO; GO:0031505; P:fungal-type cell wall organization; IMP:SGD.
DR   GO; GO:0009408; P:response to heat; IMP:SGD.
DR   GO; GO:0007266; P:Rho protein signal transduction; IMP:SGD.
DR   InterPro; IPR051694; Immunoregulatory_rcpt-like.
DR   InterPro; IPR002889; WSC_carb-bd.
DR   PANTHER; PTHR15549:SF26; AXIAL BUDDING PATTERN PROTEIN 2-RELATED; 1.
DR   PANTHER; PTHR15549; PAIRED IMMUNOGLOBULIN-LIKE TYPE 2 RECEPTOR; 1.
DR   Pfam; PF01822; WSC; 1.
DR   SMART; SM00321; WSC; 1.
DR   PROSITE; PS51212; WSC; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Cell wall biogenesis/degradation; Glycoprotein; Membrane;
KW   Phosphoprotein; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..503
FT                   /note="Cell wall integrity and stress response component 2"
FT                   /id="PRO_0000041484"
FT   TOPO_DOM        24..325
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        326..346
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        347..503
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          25..118
FT                   /note="WSC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00558"
FT   REGION          124..260
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          470..503
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         402
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
FT   MOD_RES         455
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950,
FT                   ECO:0007744|PubMed:19779198"
FT   MOD_RES         458
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:17330950,
FT                   ECO:0007744|PubMed:19779198"
SQ   SEQUENCE   503 AA;  52292 MW;  F2392A73C5CBAB50 CRC64;
     MHLDLIHKSF ILVWLIYIRA ALADQFTYKA CYSASDIRKL GLTYKGVYEY QSVSYCQNEC
     PGQAVVALFN GTGCYCGGSV AQLQSLTQVD SSKCDVSCAG WPYQNCGGSS AMNVYINNAA
     STADSTSSTA TSTSTTSSSS TSVSSKTSTK LDTKTSTSSS ATHSSSSSST TSTTTSSSET
     TTSSSSSSSS SSTSTTSTTS TTSSTTSTSS SPSTTSSSTS ASSSSETSST QATSSSTTST
     SSSTSTATVT STPSSTSIGT STHYTTRVVT QSVVSQANQQ ASTIFTTRTS VYATVSSTSS
     STSSLLNGKS SSSKSKGLSG GAIAGVVVGV VCGTVALLAL ALFFFVWKKR RQSSQHVDLE
     ETKQYQPYSL GDADANPVIP PSASSTNWHI PSRNNTALSK NTASTFATYD LPTRAPGGRD
     SIITGDAHNI SKRSHFPSVV YEEPPSIYNG NQRFSATSLP DMMEERQLHI VNPDNVSSNI
     GSNVSDGDDD YDDAKDSNNS SLR
//