ID   PSY_NARPS               Reviewed;         423 AA.
AC   P53797;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   02-SEP-2026, entry version 94.
DE   RecName: Full=Phytoene synthase, chloroplastic;
DE            EC=2.5.1.32 {ECO:0000250|UniProtKB:P37272};
DE   Flags: Precursor;
GN   Name=PSY;
OS   Narcissus pseudonarcissus (Daffodil).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Amaryllidaceae;
OC   Amaryllidoideae; Narcissus.
OX   NCBI_TaxID=39639;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Paracorolla;
RA   Schledz M., Beyer P.;
RT   "Nucleotide sequence of a Narcissus pseudonarcissus cDNA for phytoene
RT   synthase.";
RL   (er) Plant Gene Register PGR95-122(1995).
CC   -!- FUNCTION: Catalyzes the reaction from prephytoene diphosphate to
CC       phytoene. {ECO:0000250|UniProtKB:P37272}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC         diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:34476;
CC         Evidence={ECO:0000250|UniProtKB:P37272};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = (1R,2R,3R)-
CC         prephytoene diphosphate + diphosphate; Xref=Rhea:RHEA:22296,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58011, ChEBI:CHEBI:58756;
CC         Evidence={ECO:0000250|UniProtKB:P37272};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22297;
CC         Evidence={ECO:0000250|UniProtKB:P37272};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(1R,2R,3R)-prephytoene diphosphate = 15-cis-phytoene +
CC         diphosphate; Xref=Rhea:RHEA:34479, ChEBI:CHEBI:27787,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58011;
CC         Evidence={ECO:0000250|UniProtKB:P37272};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:34480;
CC         Evidence={ECO:0000250|UniProtKB:P37272};
CC   -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis; all-trans-
CC       phytoene from geranylgeranyl diphosphate: step 1/1.
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:P37272}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC       {ECO:0000250|UniProtKB:P49293}. Plastid, chromoplast
CC       {ECO:0000250|UniProtKB:P49293}.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC       {ECO:0000305}.
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DR   EMBL; X78814; CAA55391.1; -; mRNA.
DR   PIR; S54135; S54135.
DR   AlphaFoldDB; P53797; -.
DR   SMR; P53797; -.
DR   BioCyc; MetaCyc:MONOMER-18247; -.
DR   UniPathway; UPA00799; UER00773.
DR   GO; GO:0009509; C:chromoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0010287; C:plastoglobule; IEA:UniProtKB-ARBA.
DR   GO; GO:0046905; F:15-cis-phytoene synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004311; F:geranylgeranyl diphosphate synthase activity; IEA:InterPro.
DR   GO; GO:0051996; F:squalene synthase [NAD(P)H] activity; IEA:InterPro.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-ARBA.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   FunFam; 1.10.600.10:FF:000004; Phytoene synthase chloroplastic; 1.
DR   Gene3D; 1.10.600.10; Farnesyl Diphosphate Synthase; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   PANTHER; PTHR31480; BIFUNCTIONAL LYCOPENE CYCLASE/PHYTOENE SYNTHASE; 1.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDS00005; Isoprenoid_Synthase_Type_I; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1.
DR   SUPFAM; SSF48576; Terpenoid synthases; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   2: Evidence at transcript level;
KW   Carotenoid biosynthesis; Chloroplast; Isoprene biosynthesis; Plastid;
KW   Transferase; Transit peptide.
FT   TRANSIT         1..136
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           137..423
FT                   /note="Phytoene synthase, chloroplastic"
FT                   /id="PRO_0000029859"
SQ   SEQUENCE   423 AA;  47806 MW;  8E76BA35312B73DF CRC64;
     MVVAILRVVS AIEIPIRLGF SEANWRFSSP KYDNLGRKKS RLSVYSLYTT SKYACVGFEA
     ENNGKFLIRS SLVANPAGEA TISSEQKVYD VVLKQAALVK DQTKSSRKST DVKPDIVLPG
     TVYLLKDAYD RCGEVCAEYA KTFYLGTLLM TPERRRAIWA IYVWCRRTDE LVDGHNASHI
     TPSALDRWEA RLEDLFAGRP YDMFDAALSD TVSRFPVDIQ PFMDMVEGMR MDLKKSRYKN
     FDELYLYCYY VAGTVGLMSV PVMGIAPESL AEAESVYNAA LALGIANQLT NILRDVGEDA
     RRGRIYLPQD ELAEAGLSDE DVFTGKVTDK WRSFMKRQIK RARTFFEQAE KGVTELSQAS
     RWPVWASLLL YRQILDEIEA NDYNNFTKRA YVSKVKRLAA LPLAYGKSLL IPLSLRPPSL
     SKA
//