ID   SC6B2_YEAST             Reviewed;          88 AA.
AC   P52871; D3DLR8; Q53X22;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   04-NOV-2026, entry version 178.
DE   RecName: Full=Protein transport protein SBH2;
DE   AltName: Full=Ssh1 complex subunit SBH2;
DE   AltName: Full=Ssh1 complex subunit beta;
GN   Name=SBH2; Synonyms=SEB2; OrderedLocusNames=YER019C-A;
GN   ORFNames=YER019BC;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8740416;
RX   DOI=10.1002/(sici)1097-0061(199604)12:5%3c425::aid-yea924%3e3.0.co;2-b;
RA   Toikkanen J., Gatti E., Takei K., Saloheimo M., Olkkonen V.M.,
RA   Soederlund H., de Camilli P., Keraenen S.;
RT   "Yeast protein translocation complex: isolation of two genes SEB1 and SEB2
RT   encoding proteins homologous to the Sec61 beta subunit.";
RL   Yeast 12:425-438(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Finke K., Panzner S., Sommer T., Hartmann E., Rapoport T.A.;
RT   "Yeast Sec61 beta subunit.";
RL   Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169868; DOI=10.1038/387s078;
RA   Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA   Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA   Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA   Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA   Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA   Botstein D., Davis R.W.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL   Nature 387:78-81(1997).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [6]
RP   PROTEIN SEQUENCE OF 16-32.
RX   PubMed=8612571; DOI=10.1002/j.1460-2075.1996.tb00492.x;
RA   Finke K., Plath K., Panzner S., Prehn S., Rapoport T.A., Hartmann E.,
RA   Sommer T.;
RT   "A second trimeric complex containing homologs of the Sec61p complex
RT   functions in protein transport across the ER membrane of S. cerevisiae.";
RL   EMBO J. 15:1482-1494(1996).
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Part of the Ssh1 complex, which probably is the major
CC       component of a channel-forming translocon complex that may function
CC       exclusively in the cotranslational pathway of protein endoplasmic
CC       reticulum (ER) import.
CC   -!- SUBUNIT: Component of the heterotrimeric Ssh1 complex, which is
CC       composed of SSH1, SBH2 and SSS1.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC       membrane protein.
CC   -!- MISCELLANEOUS: Present with 414 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the SEC61-beta family. {ECO:0000305}.
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DR   EMBL; Z50012; CAA90305.1; -; mRNA.
DR   EMBL; L38891; AAT01101.1; -; mRNA.
DR   EMBL; U18778; AAB64567.1; -; Genomic_DNA.
DR   EMBL; AY693199; AAT93218.1; -; Genomic_DNA.
DR   EMBL; BK006939; DAA07672.1; -; Genomic_DNA.
DR   PIR; S68163; S68163.
DR   RefSeq; NP_010936.1; NM_001180031.1.
DR   PDB; 2WW9; EM; 8.60 A; C=1-87.
DR   PDB; 2WWA; EM; 8.90 A; C=1-87.
DR   AlphaFoldDB; P52871; -.
DR   SMR; P52871; -.
DR   BioGRID; 36753; 82.
DR   ComplexPortal; CPX-1834; SSH1 translocon complex.
DR   DIP; DIP-1708N; -.
DR   FunCoup; P52871; 191.
DR   IntAct; P52871; 6.
DR   STRING; 4932.YER019C-A; -.
DR   TCDB; 3.A.5.8.1; the general secretory pathway (sec) family.
DR   iPTMnet; P52871; -.
DR   PaxDb; 4932-YER019C-A; -.
DR   PeptideAtlas; P52871; -.
DR   TopDownProteomics; P52871; -.
DR   EnsemblFungi; YER019C-A_mRNA; YER019C-A; YER019C-A.
DR   GeneID; 856740; -.
DR   KEGG; sce:YER019C-A; -.
DR   AGR; SGD:S000002127; -.
DR   SGD; S000002127; SBH2.
DR   VEuPathDB; FungiDB:YER019C-A; -.
DR   eggNOG; KOG3457; Eukaryota.
DR   HOGENOM; CLU_133423_1_1_1; -.
DR   InParanoid; P52871; -.
DR   OMA; AIHFMNK; -.
DR   OrthoDB; 5401193at2759; -.
DR   BioCyc; YEAST:G3O-30348-MONOMER; -.
DR   Reactome; R-SCE-9609523; Insertion of tail-anchored proteins into the endoplasmic reticulum membrane.
DR   BioGRID-ORCS; 856740; 5 hits in 10 CRISPR screens.
DR   EvolutionaryTrace; P52871; -.
DR   PRO; PR:P52871; -.
DR   Proteomes; UP000002311; Chromosome V.
DR   RNAct; P52871; protein.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; NAS:ComplexPortal.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005637; C:nuclear inner membrane; IDA:SGD.
DR   GO; GO:0005784; C:Sec61 translocon complex; IEA:InterPro.
DR   GO; GO:0071261; C:Ssh1 translocon complex; IDA:SGD.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:SGD.
DR   GO; GO:0031204; P:post-translational protein targeting to membrane, translocation; IBA:GO_Central.
DR   GO; GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; IGI:SGD.
DR   GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IBA:GO_Central.
DR   InterPro; IPR030671; Sec61-beta/Sbh.
DR   InterPro; IPR016482; SecG/Sec61-beta/Sbh.
DR   PANTHER; PTHR13509; SEC61 SUBUNIT BETA; 1.
DR   Pfam; PF03911; Sec61_beta; 1.
DR   PIRSF; PIRSF006398; Sec61_beta_euk; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Endoplasmic reticulum; Membrane;
KW   Protein transport; Reference proteome; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..88
FT                   /note="Protein transport protein SBH2"
FT                   /id="PRO_0000157262"
FT   TOPO_DOM        1..61
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        62..82
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..42
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        28..42
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   88 AA;  9606 MW;  5A4F5B9184202E88 CRC64;
     MAASVPPGGQ RILQKRRQAQ SIKEKQAKQT PTSTRQAGYG GSSSSILKLY TDEANGFRVD
     SLVVLFLSVG FIFSVIALHL LTKFTHII
//