ID   PNTAB_RICPR             Reviewed;         132 AA.
AC   P51995;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 2.
DT   28-JAN-2026, entry version 107.
DE   RecName: Full=NAD(P) transhydrogenase subunit alpha part 2;
DE            EC=7.1.1.1 {ECO:0000250|UniProtKB:Q2RSB2};
DE   AltName: Full=Nicotinamide nucleotide transhydrogenase subunit alpha 2;
DE   AltName: Full=Pyridine nucleotide transhydrogenase subunit alpha 2;
GN   Name=pntAB; OrderedLocusNames=RP862;
OS   Rickettsia prowazekii (strain Madrid E).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
OC   Rickettsiales; Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=272947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Madrid E;
RA   Wood D.O., Marks G.L., Winkler H.H.;
RL   Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9823893; DOI=10.1038/24094;
RA   Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA   Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA   Kurland C.G.;
RT   "The genome sequence of Rickettsia prowazekii and the origin of
RT   mitochondria.";
RL   Nature 396:133-140(1998).
RN   [3]
RP   IDENTIFICATION, AND GENE NAME.
RX   PubMed=8662004; DOI=10.1007/bf02352282;
RA   Andersson S.G.E., Sharp P.M.;
RT   "Codon usage and base composition in Rickettsia prowazekii.";
RL   J. Mol. Evol. 42:525-536(1996).
CC   -!- FUNCTION: The transhydrogenation between NADH and NADP is coupled to
CC       respiration and ATP hydrolysis and functions as a proton pump across
CC       the membrane. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + NADPH + H(+)(in) = NADH + NADP(+) + H(+)(out);
CC         Xref=Rhea:RHEA:47992, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:57945, ChEBI:CHEBI:58349; EC=7.1.1.1;
CC         Evidence={ECO:0000250|UniProtKB:Q2RSB2};
CC   -!- SUBUNIT: Complex of an alpha and a beta chain; in Rickettsia, the alpha
CC       chain seems to be made of two subunits.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
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DR   EMBL; U02878; -; NOT_ANNOTATED_CDS; Unassigned_DNA.
DR   EMBL; AJ235273; CAA15286.1; -; Genomic_DNA.
DR   PIR; F71648; F71648.
DR   RefSeq; WP_004596754.1; NC_000963.1.
DR   AlphaFoldDB; P51995; -.
DR   SMR; P51995; -.
DR   STRING; 272947.gene:17555931; -.
DR   EnsemblBacteria; CAA15286; CAA15286; CAA15286.
DR   GeneID; 57569985; -.
DR   KEGG; rpr:RP862; -.
DR   PATRIC; fig|272947.5.peg.901; -.
DR   eggNOG; COG3288; Bacteria.
DR   HOGENOM; CLU_137885_0_0_5; -.
DR   OrthoDB; 9810841at2; -.
DR   Proteomes; UP000002480; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0050661; F:NADP binding; IEA:TreeGrafter.
DR   GO; GO:0008750; F:proton-translocating NAD(P)+ transhydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006740; P:NADPH regeneration; IEA:TreeGrafter.
DR   InterPro; IPR024605; NADP_transhyd_a_C.
DR   PANTHER; PTHR10160; NAD(P) TRANSHYDROGENASE; 1.
DR   PANTHER; PTHR10160:SF19; PROTON-TRANSLOCATING NAD(P)(+) TRANSHYDROGENASE; 1.
DR   Pfam; PF12769; PNTB_4TM; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; NAD; NADP;
KW   Reference proteome; Translocase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..132
FT                   /note="NAD(P) transhydrogenase subunit alpha part 2"
FT                   /id="PRO_0000199022"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        103..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   132 AA;  14325 MW;  8A56163C9F394F0D CRC64;
     MNQLPIMAKQ AAEIASNAQE LSNKLKDLVI DASWQTNTNT IDPLVFAITI FVLASFVGYY
     VVWKVTPALH TPLMSITNAI SGIIVISSMI AITSSSAFEF SSLLGSFATL LASINIFGGF
     IVTTRMLEMF KK
//