ID SH2B3_RAT Reviewed; 368 AA. AC P50745; DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot. DT 01-OCT-1996, sequence version 1. DT 04-NOV-2026, entry version 138. DE RecName: Full=SH2B adapter protein 3; DE AltName: Full=Lymphocyte adapter protein; DE AltName: Full=Lymphocyte-specific adapter protein Lnk; DE AltName: Full=Signal transduction protein Lnk; GN Name=Sh2b3; Synonyms=Lnk; OS Rattus norvegicus (Rat). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Rattus. OX NCBI_TaxID=10116; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LNK1; LNK2; LNK3 AND LNK4). RC STRAIN=Fischer; TISSUE=Lymph node; RX PubMed=8524815; DOI=10.1073/pnas.92.25.11618; RA Huang X., Li Y., Tanaka K., Moore K.G., Hayashi J.; RT "Cloning and characterization of Lnk, a signal transduction protein that RT links T-cell receptor activation signal to phospholipase C gamma 1, Grb2, RT and phosphatidylinositol 3-kinase."; RL Proc. Natl. Acad. Sci. U.S.A. 92:11618-11622(1995). CC -!- FUNCTION: Links T-cell receptor activation signal to phospholipase C- CC gamma-1, GRB2 and phosphatidylinositol 3-kinase. CC -!- ALTERNATIVE PRODUCTS: CC Event=Alternative splicing; Named isoforms=4; CC Name=Lnk4; CC IsoId=P50745-1; Sequence=Displayed; CC Name=Lnk1; CC IsoId=P50745-2; Sequence=VSP_004310; CC Name=Lnk2; CC IsoId=P50745-3; Sequence=VSP_004308, VSP_004311; CC Name=Lnk3; CC IsoId=P50745-4; Sequence=VSP_004309, VSP_004311; CC -!- TISSUE SPECIFICITY: Preferentially expressed by lymphocytes in lymph CC node and spleen. CC -!- PTM: Tyrosine-phosphorylated upon T-cell activation. The tyrosine CC phosphorylation site is multifunctional and may associate with the SH2 CC domains of phospholipase C-gamma-1, GRB-2, and PI3-K upon T-cell CC receptor activation. CC -!- SIMILARITY: Belongs to the SH2B adapter family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; U24655; AAC52351.1; -; mRNA. DR EMBL; U24654; AAC52350.1; -; mRNA. DR EMBL; U24653; AAC52349.1; -; mRNA. DR EMBL; U24652; AAC52348.1; -; mRNA. DR RefSeq; XP_063127675.1; XM_063271605.1. [P50745-3] DR PDB; 8CZ9; X-ray; 1.65 A; A=111-220. DR AlphaFoldDB; P50745; -. DR SMR; P50745; -. DR BioGRID; 248640; 2. DR FunCoup; P50745; 8. DR STRING; 10116.ENSRNOP00000038125; -. DR PhosphoSitePlus; P50745; -. DR PaxDb; 10116-ENSRNOP00000038125; -. DR Ensembl; ENSRNOT00000143806.1; ENSRNOP00000110469.1; ENSRNOG00000028198.7. [P50745-3] DR UCSC; RGD:68411; rat. [P50745-1] DR AGR; RGD:68411; -. DR RGD; 68411; Sh2b3. DR eggNOG; ENOG502QS89; Eukaryota. DR GeneTree; ENSGT00950000183191; -. DR HOGENOM; CLU_014885_3_0_1; -. DR InParanoid; P50745; -. DR PhylomeDB; P50745; -. DR PRO; PR:P50745; -. DR Proteomes; UP000002494; Chromosome 12. DR GO; GO:0042301; F:phosphate ion binding; IDA:RGD. DR GO; GO:1990782; F:protein tyrosine kinase binding; ISO:RGD. DR GO; GO:0044877; F:protein-containing complex binding; IDA:RGD. DR GO; GO:0030159; F:signaling receptor complex adaptor activity; ISO:RGD. DR GO; GO:0005173; F:stem cell factor receptor binding; ISO:RGD. DR GO; GO:0030154; P:cell differentiation; ISO:RGD. DR GO; GO:1990869; P:cellular response to chemokine; ISO:RGD. DR GO; GO:0036016; P:cellular response to interleukin-3; ISO:RGD. DR GO; GO:0035162; P:embryonic hemopoiesis; ISO:RGD. DR GO; GO:0048821; P:erythrocyte development; ISO:RGD. DR GO; GO:0030097; P:hemopoiesis; ISO:RGD. DR GO; GO:0035556; P:intracellular signal transduction; ISO:RGD. DR GO; GO:0035855; P:megakaryocyte development; ISO:RGD. DR GO; GO:0035702; P:monocyte homeostasis; ISO:RGD. DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:RGD. DR GO; GO:0070100; P:negative regulation of chemokine-mediated signaling pathway; ISO:RGD. DR GO; GO:1900235; P:negative regulation of Kit signaling pathway; ISO:RGD. DR GO; GO:0043409; P:negative regulation of MAPK cascade; ISO:RGD. DR GO; GO:0051898; P:negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; ISO:RGD. DR GO; GO:0090331; P:negative regulation of platelet aggregation; ISO:RGD. DR GO; GO:0046426; P:negative regulation of receptor signaling pathway via JAK-STAT; ISO:RGD. DR GO; GO:1904893; P:negative regulation of receptor signaling pathway via STAT; ISO:RGD. DR GO; GO:0060761; P:negative regulation of response to cytokine stimulus; ISO:RGD. DR GO; GO:1903671; P:negative regulation of sprouting angiogenesis; IMP:RGD. DR GO; GO:0001780; P:neutrophil homeostasis; ISO:RGD. DR GO; GO:0045589; P:regulation of regulatory T cell differentiation; IMP:RGD. DR GO; GO:0038163; P:thrombopoietin-mediated signaling pathway; ISO:RGD. DR CDD; cd10412; SH2_SH2B3; 1. DR FunFam; 3.30.505.10:FF:000008; SH2B adapter protein 1 isoform 2; 1. DR Gene3D; 3.30.505.10; SH2 domain; 1. DR InterPro; IPR000980; SH2. DR InterPro; IPR036860; SH2_dom_sf. DR InterPro; IPR030523; SH2B. DR InterPro; IPR035059; SH2B3_SH2. DR PANTHER; PTHR10872; SH2B ADAPTER PROTEIN; 1. DR PANTHER; PTHR10872:SF1; SH2B ADAPTER PROTEIN 3; 1. DR Pfam; PF00017; SH2; 1. DR PRINTS; PR00401; SH2DOMAIN. DR SMART; SM00252; SH2; 1. DR SUPFAM; SSF55550; SH2 domain; 1. DR PROSITE; PS50001; SH2; 1. PE 1: Evidence at protein level; KW 3D-structure; Alternative splicing; Phosphoprotein; Reference proteome; KW SH2 domain. FT CHAIN 1..368 FT /note="SH2B adapter protein 3" FT /id="PRO_0000084456" FT DOMAIN 119..217 FT /note="SH2" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00191" FT MOD_RES 356 FT /note="Phosphotyrosine" FT /evidence="ECO:0000255" FT VAR_SEQ 1..95 FT /note="MAGRTQGKHRPRVRTCPSLVPFDNPCWPPSHQPLPLQVGAPGHRVTSFLSGR FT AWPSYIPKGKHGLPGPRYVPDPWSLGVLGHVAPLRVTTTIFLP -> MPDNLYTFVLKV FT QGQTDIIFEVGDEQQLNSWLAELRASTGLGLEHLDTELPLSLVAEPGPAISPRGSTDSL FT DQ (in isoform Lnk2)" FT /evidence="ECO:0000303|PubMed:8524815" FT /id="VSP_004308" FT VAR_SEQ 14..38 FT /note="Missing (in isoform Lnk3)" FT /evidence="ECO:0000303|PubMed:8524815" FT /id="VSP_004309" FT VAR_SEQ 225..278 FT /note="Missing (in isoform Lnk1)" FT /evidence="ECO:0000303|PubMed:8524815" FT /id="VSP_004310" FT VAR_SEQ 226..262 FT /note="Missing (in isoform Lnk2 and isoform Lnk3)" FT /evidence="ECO:0000303|PubMed:8524815" FT /id="VSP_004311" SQ SEQUENCE 368 AA; 40432 MW; 624CC2ABA0332C87 CRC64; MAGRTQGKHR PRVRTCPSLV PFDNPCWPPS HQPLPLQVGA PGHRVTSFLS GRAWPSYIPK GKHGLPGPRY VPDPWSLGVL GHVAPLRVTT TIFLPGASPG VMLDPACQKT DHFLSCYPWF HGPISRVRAA QLVQLQGPDA HGVFLVRQSE SRRGEYVLTF NLQGRAKHLR LVLTERGQCR VQHLHFPSVV DMLRHFQRSP IPLECGAACD VRLSGYVVVV SQAPGRKPKV VSRGGVTAST ARTKLPSWLR LLHGVIHVIS PGSSNTVLFP FSLPHWDSEL GHPHLSSAGC PPGHGAEALR GQVTPPEQIF HLVPSPEELA NSLRQLELES VSSARDSDYE MDSSSRSHLR AIDNQYTPLS QLCREANL //