ID   UCRI1_TOBAC             Reviewed;         258 AA.
AC   P49729;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   02-SEP-2026, entry version 117.
DE   RecName: Full=Cytochrome b-c1 complex subunit Rieske-1, mitochondrial;
DE            EC=7.1.1.8;
DE   AltName: Full=Complex III subunit 5-1;
DE   AltName: Full=Rieske iron-sulfur protein 1;
DE            Short=RISP1;
DE   AltName: Full=Ubiquinol-cytochrome c reductase iron-sulfur subunit 1;
DE   Flags: Precursor; Fragment;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   PubMed=1961737; DOI=10.1073/pnas.88.23.10716;
RA   Huang J.T., Struck F., Matzinger D.F., Levings C.S. III;
RT   "Functional analysis in yeast of cDNA coding for the mitochondrial Rieske
RT   iron-sulfur protein of higher plants.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:10716-10720(1991).
CC   -!- FUNCTION: Component of the ubiquinol-cytochrome c oxidoreductase, a
CC       multisubunit transmembrane complex that is part of the mitochondrial
CC       electron transport chain which drives oxidative phosphorylation. The
CC       respiratory chain contains 3 multisubunit complexes succinate
CC       dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase
CC       (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase
CC       (complex IV, CIV), that cooperate to transfer electrons derived from
CC       NADH and succinate to molecular oxygen, creating an electrochemical
CC       gradient over the inner membrane that drives transmembrane transport
CC       and the ATP synthase. The cytochrome b-c1 complex catalyzes electron
CC       transfer from ubiquinol to cytochrome c, linking this redox reaction to
CC       translocation of protons across the mitochondrial inner membrane, with
CC       protons being carried across the membrane as hydrogens on the quinol.
CC       In the process called Q cycle, 2 protons are consumed from the matrix,
CC       4 protons are released into the intermembrane space and 2 electrons are
CC       passed to cytochrome c. The Rieske protein is a catalytic core subunit
CC       containing a [2Fe-2S] iron-sulfur cluster. It cycles between 2
CC       conformational states during catalysis to transfer electrons from the
CC       quinol bound in the Q(0) site in cytochrome b to cytochrome c1.
CC       {ECO:0000250|UniProtKB:P08067}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2
CC         Fe(II)-[cytochrome c](out) + 2 H(+)(out); Xref=Rhea:RHEA:11484,
CC         Rhea:RHEA-COMP:10350, Rhea:RHEA-COMP:14399, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:24646, ChEBI:CHEBI:29033, ChEBI:CHEBI:29034,
CC         ChEBI:CHEBI:132124; EC=7.1.1.8;
CC         Evidence={ECO:0000250|UniProtKB:P08067};
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU00628};
CC       Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000255|PROSITE-
CC       ProRule:PRU00628};
CC   -!- SUBUNIT: Component of the ubiquinol-cytochrome c oxidoreductase
CC       (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme
CC       composed of 3 respiratory subunits cytochrome b, cytochrome c1 and
CC       Rieske protein, 2 core protein subunits, and several low-molecular
CC       weight protein subunits. The complex exists as an obligatory dimer and
CC       forms supercomplexes (SCs) in the inner mitochondrial membrane with
CC       cytochrome c oxidase (complex IV, CIV). {ECO:0000250|UniProtKB:P08067}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:P08067}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:P08067}.
CC   -!- MISCELLANEOUS: The Rieske protein is a high potential 2Fe-2S protein.
CC   -!- SIMILARITY: Belongs to the Rieske iron-sulfur protein family.
CC       {ECO:0000305}.
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DR   EMBL; M77225; AAA34112.1; -; mRNA.
DR   PIR; B41607; B41607.
DR   AlphaFoldDB; P49729; -.
DR   SMR; P49729; -.
DR   STRING; 4097.P49729; -.
DR   PaxDb; 4097-P49729; -.
DR   Proteomes; UP000790787; Unplaced.
DR   GO; GO:0005743; C:mitochondrial inner membrane; ISS:UniProtKB.
DR   GO; GO:0005739; C:mitochondrion; IDA:AgBase.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:InterPro.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0008121; F:quinol-cytochrome-c reductase activity; IGI:AgBase.
DR   GO; GO:0009060; P:aerobic respiration; IGI:AgBase.
DR   GO; GO:0006122; P:mitochondrial electron transport, ubiquinol to cytochrome c; IBA:GO_Central.
DR   GO; GO:0009408; P:response to heat; IGI:AgBase.
DR   CDD; cd03470; Rieske_cytochrome_bc1; 1.
DR   FunFam; 2.102.10.10:FF:000001; Cytochrome b-c1 complex subunit Rieske, mitochondrial; 1.
DR   Gene3D; 2.102.10.10; Rieske [2Fe-2S] iron-sulphur domain; 1.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR   InterPro; IPR014349; Rieske_Fe-S_prot.
DR   InterPro; IPR005805; Rieske_Fe-S_prot_C.
DR   InterPro; IPR004192; Rieske_TM.
DR   InterPro; IPR006317; Ubiquinol_cyt_c_Rdtase_Fe-S-su.
DR   NCBIfam; TIGR01416; Rieske_proteo; 1.
DR   PANTHER; PTHR10134; CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL; 1.
DR   Pfam; PF00355; Rieske; 1.
DR   Pfam; PF02921; UCR_TM; 1.
DR   PRINTS; PR00162; RIESKE.
DR   SUPFAM; SSF50022; ISP domain; 1.
DR   SUPFAM; SSF81502; ISP transmembrane anchor; 1.
DR   PROSITE; PS51296; RIESKE; 1.
PE   2: Evidence at transcript level;
KW   2Fe-2S; Disulfide bond; Electron transport; Iron; Iron-sulfur; Membrane;
KW   Metal-binding; Mitochondrion; Mitochondrion inner membrane;
KW   Reference proteome; Respiratory chain; Transit peptide; Translocase;
KW   Transmembrane; Transmembrane helix; Transport.
FT   TRANSIT         <1..46
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250"
FT   CHAIN           47..258
FT                   /note="Cytochrome b-c1 complex subunit Rieske-1,
FT                   mitochondrial"
FT                   /id="PRO_0000030676"
FT   TOPO_DOM        47..95
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        96..118
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        119..258
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000305"
FT   DOMAIN          161..256
FT                   /note="Rieske"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         201
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         203
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         220
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   BINDING         223
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   DISULFID        206..222
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT   NON_TER         1
SQ   SEQUENCE   258 AA;  28192 MW;  FDA51B570CCEDCAF CRC64;
     WPVRSAAPSS SAFISANHFS SDDDSSSPRS ISPSLASVFL HHTRGFSSNS VSPAHDMGLV
     PDLPPTVAAI KNPTSKIVYD EHNHERYPPG DPSKRAFAYF VLTGGRFVYA SLMRLLILKF
     VLSMSASKDV LALASLEVDL SSIEPGTTVT VKWRGKPVFI RRRTEDDISL ANSVDLGSLR
     DPQQDAERVK NPEWLVVIGV CTHLGCIPLP NAGDFGGWFC PCHGSHYDIS GRIRKGPAPY
     NLEVPTYSFL EENKLLIG
//