ID   CYB5_ORYSJ              Reviewed;         137 AA.
AC   P49100; Q0DLC5; Q65X19; Q94HK6;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 2.
DT   02-SEP-2026, entry version 149.
DE   RecName: Full=Cytochrome b5;
GN   OrderedLocusNames=Os05g0108800, LOC_Os05g01820;
GN   ORFNames=OsJ_16839 {ECO:0000312|EMBL:EEE62055.1}, OSJNBa0068N01.16;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Callus;
RX   PubMed=8049375; DOI=10.1007/bf00043880;
RA   Smith M.A., Stobart A.K., Shewry P.R., Napier J.A.;
RT   "Tobacco cytochrome b5: cDNA isolation, expression analysis and in vitro
RT   protein targeting.";
RL   Plant Mol. Biol. 25:527-537(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16261349; DOI=10.1007/s00438-005-0039-y;
RA   Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J.,
RA   Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F.,
RA   Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y.;
RT   "A fine physical map of the rice chromosome 5.";
RL   Mol. Genet. Genomics 274:337-345(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA   Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA   Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA   Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA   Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA   Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA   Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA   Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA   Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA   Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA   Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA   Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA   Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA   Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA   McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT   "The genomes of Oryza sativa: a history of duplications.";
RL   PLoS Biol. 3:266-281(2005).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: Membrane bound hemoprotein which function as an electron
CC       carrier for several membrane bound oxygenases. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}; Cytoplasmic side
CC       {ECO:0000250}. Microsome membrane {ECO:0000250}; Single-pass membrane
CC       protein {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the cytochrome b5 family. {ECO:0000305}.
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DR   EMBL; X75670; CAA53366.1; -; mRNA.
DR   EMBL; AC079022; AAK73138.1; -; Genomic_DNA.
DR   EMBL; AC129716; AAU44139.1; -; Genomic_DNA.
DR   EMBL; AP008211; BAF16348.1; -; Genomic_DNA.
DR   EMBL; AP014961; BAS91901.1; -; Genomic_DNA.
DR   EMBL; CM000142; EEE62055.1; -; Genomic_DNA.
DR   EMBL; AK064821; BAG89223.1; -; mRNA.
DR   PIR; S46307; S46307.
DR   AlphaFoldDB; P49100; -.
DR   SMR; P49100; -.
DR   FunCoup; P49100; 3046.
DR   STRING; 39947.P49100; -.
DR   PaxDb; 39947-P49100; -.
DR   EnsemblPlants; Os05t0108800-01; Os05t0108800-01; Os05g0108800.
DR   Gramene; Os05t0108800-01; Os05t0108800-01; Os05g0108800.
DR   KEGG; dosa:Os05g0108800; -.
DR   eggNOG; KOG0537; Eukaryota.
DR   HOGENOM; CLU_102602_3_0_1; -.
DR   InParanoid; P49100; -.
DR   OMA; HNTRNDL; -.
DR   OrthoDB; 260519at2759; -.
DR   Proteomes; UP000059680; Chromosome 5.
DR   ExpressionAtlas; P49100; baseline and differential.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0020037; F:heme binding; IBA:GO_Central.
DR   FunFam; 3.10.120.10:FF:000002; Cytochrome b5 type B; 1.
DR   Gene3D; 3.10.120.10; Cytochrome b5-like heme/steroid binding domain; 1.
DR   InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR   InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR   InterPro; IPR018506; Cyt_B5_heme-BS.
DR   InterPro; IPR050668; Cytochrome_b5.
DR   PANTHER; PTHR19359; CYTOCHROME B5; 1.
DR   PANTHER; PTHR19359:SF129; CYTOCHROME B5 ISOFORM B; 1.
DR   Pfam; PF00173; Cyt-b5; 1.
DR   PRINTS; PR00363; CYTOCHROMEB5.
DR   SMART; SM01117; Cyt-b5; 1.
DR   SUPFAM; SSF55856; Cytochrome b5-like heme/steroid binding domain; 1.
DR   PROSITE; PS00191; CYTOCHROME_B5_1; 1.
DR   PROSITE; PS50255; CYTOCHROME_B5_2; 1.
PE   2: Evidence at transcript level;
KW   Electron transport; Endoplasmic reticulum; Heme; Iron; Membrane;
KW   Metal-binding; Microsome; Reference proteome; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..137
FT                   /note="Cytochrome b5"
FT                   /id="PRO_0000166027"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          6..82
FT                   /note="Cytochrome b5 heme-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT   BINDING         41
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT   BINDING         65
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00279"
FT   CONFLICT        66
FT                   /note="S -> T (in Ref. 1; CAA53366)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   137 AA;  15282 MW;  5FBBCF0BE8E60FDE CRC64;
     MSNDNKKVYT LEEVAKHNSK DDCWLIIGGK VYNVSKFLED HPGGDDVLLS STGKDATDDF
     EDVGHSTTAR AMMDEYYVGD IDTSTIPART KYVPPKQPHY NQDKTPEFII KILQFLVPLA
     ILGLAVAIRI YTKSESA
//