ID   NDF6_MOUSE              Reviewed;         337 AA.
AC   P48986;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   02-SEP-2026, entry version 167.
DE   RecName: Full=Neurogenic differentiation factor 6;
DE            Short=NeuroD6;
DE   AltName: Full=Helix-loop-helix protein mATH-2;
DE            Short=mATH2;
DE   AltName: Full=Protein NEX-1;
DE   AltName: Full=Protein atonal homolog 2;
GN   Name=Neurod6; Synonyms=Ath2, Atoh2, Nex1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=129/J;
RX   PubMed=7744035; DOI=10.1111/j.1432-1033.1995.tb20461.x;
RA   Shimizu C., Akazawa C., Nakanishi S., Kageyama R.;
RT   "MATH-2, a mammalian helix-loop-helix factor structurally related to the
RT   product of Drosophila proneural gene atonal, is specifically expressed in
RT   the nervous system.";
RL   Eur. J. Biochem. 229:239-248(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Brain;
RX   PubMed=7545978; DOI=10.1016/0925-4773(94)90061-2;
RA   Bartholomae A., Nave K.-A.;
RT   "NEX-1: a novel brain-specific helix-loop-helix protein with autoregulation
RT   and sustained expression in mature cortical neurons.";
RL   Mech. Dev. 48:217-228(1994).
CC   -!- FUNCTION: Activates E box-dependent transcription in collaboration with
CC       TCF3/E47. May be a trans-acting factor involved in the development and
CC       maintenance of the mammalian nervous system. Transactivates the
CC       promoter of its own gene.
CC   -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC       protein.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Specific to the nervous system of both embryos and
CC       adults. Highest levels in the cortical plate of the cerebrum.
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DR   EMBL; D44480; BAA07923.1; -; Genomic_DNA.
DR   EMBL; U29086; AAC14576.1; -; mRNA.
DR   CCDS; CCDS20168.1; -.
DR   PIR; I48682; I48682.
DR   PIR; I57038; I57038.
DR   RefSeq; NP_033847.1; NM_009717.2.
DR   AlphaFoldDB; P48986; -.
DR   SMR; P48986; -.
DR   FunCoup; P48986; 1497.
DR   STRING; 10090.ENSMUSP00000047016; -.
DR   PhosphoSitePlus; P48986; -.
DR   PaxDb; 10090-ENSMUSP00000047016; -.
DR   ProteomicsDB; 293534; -.
DR   Antibodypedia; 12707; 185 antibodies from 31 providers.
DR   DNASU; 11922; -.
DR   Ensembl; ENSMUST00000044767.10; ENSMUSP00000047016.9; ENSMUSG00000037984.10.
DR   GeneID; 11922; -.
DR   KEGG; mmu:11922; -.
DR   UCSC; uc009cav.2; mouse.
DR   AGR; MGI:106593; -.
DR   CTD; 63974; -.
DR   MGI; MGI:106593; Neurod6.
DR   VEuPathDB; HostDB:ENSMUSG00000037984; -.
DR   eggNOG; KOG3898; Eukaryota.
DR   GeneTree; ENSGT00940000159827; -.
DR   HOGENOM; CLU_055134_1_0_1; -.
DR   InParanoid; P48986; -.
DR   OMA; FPYDFHL; -.
DR   OrthoDB; 10039134at2759; -.
DR   PhylomeDB; P48986; -.
DR   BioGRID-ORCS; 11922; 1 hit in 77 CRISPR screens.
DR   PRO; PR:P48986; -.
DR   Proteomes; UP000000589; Chromosome 6.
DR   RNAct; P48986; protein.
DR   Bgee; ENSMUSG00000037984; Expressed in cortical plate and 98 other cell types or tissues.
DR   ExpressionAtlas; P48986; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0070888; F:E-box binding; IBA:GO_Central.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:0061564; P:axon development; IBA:GO_Central.
DR   GO; GO:0021542; P:dentate gyrus development; IGI:MGI.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0007423; P:sensory organ development; IBA:GO_Central.
DR   CDD; cd19722; bHLH_TS_NeuroD6_ATOH2; 1.
DR   FunFam; 4.10.280.10:FF:000006; Neurogenic differentiation factor; 1.
DR   Gene3D; 4.10.280.10; Helix-loop-helix DNA-binding domain; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR050359; bHLH_transcription_factors.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   InterPro; IPR022575; NeuroD_DUF.
DR   InterPro; IPR016637; TF_bHLH_NeuroD.
DR   PANTHER; PTHR19290; BASIC HELIX-LOOP-HELIX PROTEIN NEUROGENIN-RELATED; 1.
DR   PANTHER; PTHR19290:SF9; NEUROGENIC DIFFERENTIATION FACTOR 6; 1.
DR   Pfam; PF00010; HLH; 1.
DR   Pfam; PF12533; Neuro_bHLH; 1.
DR   PIRSF; PIRSF015618; bHLH_NeuroD; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; HLH, helix-loop-helix DNA-binding domain; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   2: Evidence at transcript level;
KW   Activator; Developmental protein; Differentiation; DNA-binding;
KW   Neurogenesis; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..337
FT                   /note="Neurogenic differentiation factor 6"
FT                   /id="PRO_0000127394"
FT   DOMAIN          94..146
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          28..80
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           80..86
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        54..71
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   337 AA;  38644 MW;  35C18ACD8EE1EFBA CRC64;
     MLTLPFDESV VMPESQMCRK FARQCEDQKQ IKKPESFPKQ VVLRGKSIKR APGEETEKEE
     EEEDREEEDE NGLSRRRGLR KKKTTKLRLE RVKFRRQEAN ARERNRMHGL NDALDNLRKV
     VPCYSKTQKL SKIETLRLAK NYIWALSEIL RIGKRPDLLT FVQNLCKGLS QPTTNLVAGC
     LQLNARSFLM GQGGEAAHHT RSPYSTFYPP YHSPELATPP GHGTLDNSKS MKPYNYCSAY
     ESFYESTSPE CASPQFEGPL SPPPINYNGI FSLKQEETLD YGKNYNYGMH YCAVPPRGPL
     GQGAMFRLPT DSHFPYDLHL RSQSLTMQDE LNAVFHN
//