ID   CHSB_IPOTR              Reviewed;         366 AA.
AC   P48404;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   28-JAN-2026, entry version 85.
DE   RecName: Full=Chalcone synthase B;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase B;
DE            Short=CHS-B;
DE   Flags: Fragment;
GN   Name=CHSB;
OS   Ipomoea triloba (Trilobed morning glory).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Convolvulaceae; Ipomoeeae; Ipomoea.
OX   NCBI_TaxID=35885;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7724563; DOI=10.1073/pnas.92.8.3338;
RA   Durbin M.L., Learn G.H., Huttley G.A., Clegg M.T.;
RT   "Evolution of the chalcone synthase gene family in the genus Ipomoea.";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:3338-3342(1995).
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(E)-4-coumaroyl-CoA + 3 malonyl-CoA + 3 H(+) = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15413, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:85008; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; U15953; AAC49035.1; -; Genomic_DNA.
DR   AlphaFoldDB; P48404; -.
DR   SMR; P48404; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:UniProtKB-ARBA.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0030639; P:polyketide biosynthetic process; IEA:TreeGrafter.
DR   CDD; cd00831; CHS_like; 1.
DR   FunFam; 3.40.47.10:FF:000014; Chalcone synthase 1; 1.
DR   FunFam; 3.40.47.10:FF:000025; Chalcone synthase 2; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877:SF104; CHALCONE SYNTHASE; 1.
DR   PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; Thiolase-like; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..>366
FT                   /note="Chalcone synthase B"
FT                   /id="PRO_0000216000"
FT   ACT_SITE        172
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
FT   NON_TER         366
SQ   SEQUENCE   366 AA;  40976 MW;  E80E424EF56A9B4D CRC64;
     MSTTVTVLTD TWGRRAKRFE IEGYAKILAI GTATPANWVD QTTYPDFYFR ITNSQHLLEH
     KEKFRRICNK SKIRKRHLVL TEELLQKNPS LCTYNETSLN TRQDILVSEV PKLGKEAAMK
     AIKEWGRPIS EITHLVFCTT SGVDMPGADF RLTKLLGLNS SVKRLMMYQQ GCNAGAAMLR
     LAKDLAESNK GGRILVVCSE ITINIFRGPS LEQDDNLLAQ CLFGDGSAAM IVGTDPRPDL
     ETPLFELVSS AQTIVPNTDS HLKLTLREMG LTFHCSRAVP SVLAENVEDC LVKAFEPYGI
     SDWNSIFWVF HPGGNAIVDR VEERLGLGAQ RFRASRDVLS EYGNLTSACV LFILDEVRNK
     SKKNEQ
//