ID   CHSY_CALCH              Reviewed;         398 AA.
AC   P48385;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   28-JAN-2026, entry version 89.
DE   RecName: Full=Chalcone synthase;
DE            EC=2.3.1.74;
DE   AltName: Full=Naringenin-chalcone synthase;
GN   Name=CHS;
OS   Callistephus chinensis (China aster) (Callistemma chinense).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae; Astereae;
OC   Australasian lineages; Asterinae; Callistephus.
OX   NCBI_TaxID=13379;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=L 01; TISSUE=Petal;
RA   Henkel J., Wassenegger M., Sommer H., Forkmann G.;
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: The primary product of this enzyme is 4,2',4',6'-
CC       tetrahydroxychalcone (also termed naringenin-chalcone or chalcone)
CC       which can under specific conditions spontaneously isomerize into
CC       naringenin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(E)-4-coumaroyl-CoA + 3 malonyl-CoA + 3 H(+) = 2',4,4',6'-
CC         tetrahydroxychalcone + 3 CO2 + 4 CoA; Xref=Rhea:RHEA:11128,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15413, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57384, ChEBI:CHEBI:85008; EC=2.3.1.74;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10023};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid biosynthesis.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Chalcone/stilbene
CC       synthases family. {ECO:0000305}.
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DR   EMBL; Z67988; CAA91930.1; -; mRNA.
DR   AlphaFoldDB; P48385; -.
DR   SMR; P48385; -.
DR   UniPathway; UPA00154; -.
DR   GO; GO:0016210; F:naringenin-chalcone synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042803; F:protein homodimerization activity; IEA:UniProtKB-ARBA.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0030639; P:polyketide biosynthetic process; IEA:TreeGrafter.
DR   CDD; cd00831; CHS_like; 1.
DR   FunFam; 3.40.47.10:FF:000014; Chalcone synthase 1; 1.
DR   FunFam; 3.40.47.10:FF:000025; Chalcone synthase 2; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR012328; Chalcone/stilbene_synt_C.
DR   InterPro; IPR001099; Chalcone/stilbene_synt_N.
DR   InterPro; IPR018088; Chalcone/stilbene_synthase_AS.
DR   InterPro; IPR011141; Polyketide_synthase_type-III.
DR   InterPro; IPR016039; Thiolase-like.
DR   PANTHER; PTHR11877:SF14; CHALCONE SYNTHASE; 1.
DR   PANTHER; PTHR11877; HYDROXYMETHYLGLUTARYL-COA SYNTHASE; 1.
DR   Pfam; PF02797; Chal_sti_synt_C; 1.
DR   Pfam; PF00195; Chal_sti_synt_N; 1.
DR   PIRSF; PIRSF000451; PKS_III; 1.
DR   SUPFAM; SSF53901; Thiolase-like; 2.
DR   PROSITE; PS00441; CHALCONE_SYNTH; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Flavonoid biosynthesis; Transferase.
FT   CHAIN           1..398
FT                   /note="Chalcone synthase"
FT                   /id="PRO_0000215959"
FT   ACT_SITE        167
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10023"
SQ   SEQUENCE   398 AA;  43541 MW;  8B7D4E11B4FAFFC3 CRC64;
     MASTIDIAAI REAQRRQGPA TILAIGTATP SNCVYQADYP DYYFRITKSE HMVDLKEKFK
     RMCDKSMIRK RYMHLTEEYL KENPSLCEYM APSLDARQDV VVVEVPKLGK EAATKAIKEW
     GQPKSKITHL IFCTTSGVDM PGADYQLTKL LGLRPSVKRF MMYQQGCFAG GTVLRLAKDL
     AENNKGARVL VVCSEITAVT FRGPNDTHLD SLVGQALFGD GAAAVIVGAD PDLTTERPLF
     EMISAAQTIL PDSEGAIDGH LREVGLTFHL LKDVPGLISK NIEKALTQAF SPLGITDWNS
     IFWIAHPGGP AILDQVELKL GLKEEKMRAT RHVLSEYGNM SSACVLFIID EMRKKSAEDG
     AATTGEGLDW GVLFGFGPGL TVETVVLHSL PTTMAIAT
//