ID   SYH_MYCGE               Reviewed;         414 AA.
AC   P47281;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   04-NOV-2026, entry version 142.
DE   RecName: Full=Histidine--tRNA ligase;
DE            EC=6.1.1.21;
DE   AltName: Full=Histidyl-tRNA synthetase;
DE            Short=HisRS;
GN   Name=hisS; OrderedLocusNames=MG035;
OS   Mycoplasmoides genitalium (strain ATCC 33530 / DSM 19775 / NCTC 10195 /
OS   G37) (Mycoplasma genitalium).
OC   Bacteria; Bacillati; Mycoplasmatota; Mycoplasmoidales; Mycoplasmoidaceae;
OC   Mycoplasmoides.
OX   NCBI_TaxID=243273;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33530 / DSM 19775 / NCTC 10195 / G37;
RX   PubMed=7569993; DOI=10.1126/science.270.5235.397;
RA   Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A.,
RA   Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M.,
RA   Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L.,
RA   Nguyen D.T., Utterback T.R., Saudek D.M., Phillips C.A., Merrick J.M.,
RA   Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S.,
RA   Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.;
RT   "The minimal gene complement of Mycoplasma genitalium.";
RL   Science 270:397-403(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP +
CC         diphosphate + H(+); Xref=Rhea:RHEA:17313, Rhea:RHEA-COMP:9665,
CC         Rhea:RHEA-COMP:9689, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:57595, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78527, ChEBI:CHEBI:456215; EC=6.1.1.21;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; L43967; AAC71251.1; -; Genomic_DNA.
DR   PIR; H64203; H64203.
DR   RefSeq; WP_009885699.1; NC_000908.2.
DR   AlphaFoldDB; P47281; -.
DR   SMR; P47281; -.
DR   FunCoup; P47281; 192.
DR   STRING; 243273.MG_035; -.
DR   GeneID; 99646843; -.
DR   KEGG; mge:MG_035; -.
DR   eggNOG; COG0124; Bacteria.
DR   HOGENOM; CLU_025113_1_1_14; -.
DR   InParanoid; P47281; -.
DR   OMA; AAHMPQR; -.
DR   OrthoDB; 9800814at2; -.
DR   BioCyc; MGEN243273:G1GJ2-35-MONOMER; -.
DR   BRENDA; 6.1.1.21; 3528.
DR   Proteomes; UP000000807; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004821; F:histidine-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0006427; P:histidyl-tRNA aminoacylation; IBA:GO_Central.
DR   CDD; cd00773; HisRS-like_core; 1.
DR   FunFam; 3.30.930.10:FF:000121; Histidine--tRNA ligase; 1.
DR   FunFam; 3.40.50.800:FF:000017; Histidine--tRNA ligase chloroplastic/mitochondrial; 1.
DR   Gene3D; 3.40.50.800; Anticodon-binding domain; 1.
DR   Gene3D; 3.30.930.10; Bira Bifunctional Protein, Domain 2; 1.
DR   HAMAP; MF_00127; His_tRNA_synth; 1.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR036621; Anticodon-bd_dom_sf.
DR   InterPro; IPR015807; His-tRNA-ligase.
DR   InterPro; IPR041715; HisRS-like_core.
DR   InterPro; IPR004516; HisRS/HisZ.
DR   NCBIfam; TIGR00442; hisS; 1.
DR   PANTHER; PTHR43707:SF1; HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED; 1.
DR   PANTHER; PTHR43707; HISTIDYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF13393; tRNA-synt_His; 1.
DR   PIRSF; PIRSF001549; His-tRNA_synth; 1.
DR   SUPFAM; SSF52954; Class II aaRS ABD-related; 1.
DR   SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..414
FT                   /note="Histidine--tRNA ligase"
FT                   /id="PRO_0000136198"
SQ   SEQUENCE   414 AA;  48324 MW;  9419FD5BDB12110A CRC64;
     MNFLQKPRGV KDWFGDELVY FNWIVKKIRS LAFNWGFSEV KTPLFENAQL FQRSNANADI
     VQKELYQFFD KSQRELALRP EATTPIVRLA CENKLMQEAN FPLKLFCIGS MYRYERPQNN
     RFREHWQFSC EVFGFSNLFI FLDTLLFANS LLEALGITGY VLKINNLANF ETLSKWNKAL
     KDYLTPYKLE LTELSQKRLE KNPLRILDDK IDQKKSFVKN APKITDFLDA SAKQDSELLK
     TQLKKHNISF EWTDNLVRGL DYYTGFVFEY VKNQDTILAG GVYDNLVEEL SSNPTPALGF
     ACGIERLINC LEIDKKAFIL NTKPKQMLVI CLFEEALEEL VWLAKLWREY NQVTIYPKVI
     KVDNGIRLAN RLGYTFIGIV GKTDFDKKAI TIKNLVSKQQ TIYTWNELGE RNVF
//