ID   YJY5_YEAST              Reviewed;         510 AA.
AC   P47090; D6VWJ1; Q66R65;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   10-JUN-2026, entry version 146.
DE   RecName: Full=Uncharacterized endoplasmic reticulum membrane protein YJR015W;
GN   OrderedLocusNames=YJR015W; ORFNames=J1448;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC       {ECO:0000269|PubMed:14562095}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 639 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: To yeast SNG1. {ECO:0000305}.
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DR   EMBL; X87611; CAA60938.1; -; Genomic_DNA.
DR   EMBL; Z49515; CAA89539.1; -; Genomic_DNA.
DR   EMBL; AY723836; AAU09753.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08807.1; -; Genomic_DNA.
DR   PIR; S55204; S55204.
DR   RefSeq; NP_012549.3; NM_001181673.3.
DR   AlphaFoldDB; P47090; -.
DR   BioGRID; 33771; 49.
DR   DIP; DIP-1889N; -.
DR   FunCoup; P47090; 64.
DR   IntAct; P47090; 7.
DR   MINT; P47090; -.
DR   STRING; 4932.YJR015W; -.
DR   TCDB; 9.B.316.1.1; the nitrosoguanidine resistance or duf3533 (ngr) family.
DR   iPTMnet; P47090; -.
DR   PaxDb; 4932-YJR015W; -.
DR   PeptideAtlas; P47090; -.
DR   EnsemblFungi; YJR015W_mRNA; YJR015W; YJR015W.
DR   GeneID; 853472; -.
DR   KEGG; sce:YJR015W; -.
DR   AGR; SGD:S000003776; -.
DR   SGD; S000003776; YJR015W.
DR   VEuPathDB; FungiDB:YJR015W; -.
DR   eggNOG; ENOG502QUA0; Eukaryota.
DR   HOGENOM; CLU_020178_1_0_1; -.
DR   InParanoid; P47090; -.
DR   OMA; WTMVNLR; -.
DR   OrthoDB; 2140105at2759; -.
DR   BioCyc; YEAST:G3O-31660-MONOMER; -.
DR   BioGRID-ORCS; 853472; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P47090; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P47090; protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   InterPro; IPR022703; DUF3533.
DR   InterPro; IPR053001; MNNG_permease-like.
DR   PANTHER; PTHR34814; NITROSOGUANIDINE RESISTANCE PROTEIN SNG1; 1.
DR   PANTHER; PTHR34814:SF1; NITROSOGUANIDINE RESISTANCE PROTEIN SNG1; 1.
DR   Pfam; PF12051; DUF3533; 1.
PE   1: Evidence at protein level;
KW   Endoplasmic reticulum; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..510
FT                   /note="Uncharacterized endoplasmic reticulum membrane
FT                   protein YJR015W"
FT                   /id="PRO_0000203085"
FT   TOPO_DOM        1..89
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        90..110
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        111..123
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        124..144
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        145..312
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        313..333
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        334..349
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        350..370
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        371..381
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        382..402
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        403..416
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        417..437
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        438..474
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        475..495
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        496..510
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          38..76
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        53..66
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        67..76
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        324
FT                   /note="F -> S (in Ref. 3; AAU09753)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   510 AA;  58117 MW;  CD1AB973D0042CBF CRC64;
     MTSSLDDIEP TAYNNMEADE EYCRRNDIHD LSSVVGDAVS QGVPDMDGQT TDSSKDPEPN
     SEDKKAFPPS SGSFFSPNLQ GQRKKVLLKF VFTNCLLAII CFTMFVLFWG ALYDTSKYLH
     KVKLLVVIQE PPVVILDNNS SMVVPSISYA LPTFINKIPC DWDIYNSPTF QAKFDVNTPQ
     QVNDKVVDLV YDEKYWFAIN IKPNATETLF ESLINDTAPL FNSTLFNQVV YETGRDPTNL
     KSTILPVAQT IEEYYHTFYT LNYLPPLLTN ITQVYRYALT NNARYIAAAG KYNYEYYDHR
     PFTDRILLAP TQIGVVYCLL LTFFQFLLYG PLHVEMAKVL RPANGLIYRI AMSWFTFFFA
     SLFFCTTTAI FQVDFTKSFG RGGFVVYWMS TWLFMLAAGG ANENAVMLVI TLGPQYLGFW
     ILSFVILNIA PSFFPLALNN NVYRYGYMMP VHNVIDIYRV IFFDVTRRKM GRNYGILVAL
     IALNTALLPF VGKYASRKLK QKALVAAKQS
//