ID   CFA_CITFR               Reviewed;          89 AA.
AC   P45509;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   02-SEP-2026, entry version 72.
DE   RecName: Full=Cyclopropane-fatty-acyl-phospholipid synthase;
DE            Short=CFA synthase;
DE            Short=Cyclopropane fatty acid synthase;
DE            EC=2.1.1.79;
DE   Flags: Fragment;
GN   Name=cfa;
OS   Citrobacter freundii.
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Enterobacterales; Enterobacteriaceae; Citrobacter;
OC   Citrobacter freundii complex.
OX   NCBI_TaxID=546;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 6750 / DSM 30040 / NCIB 8173 / M8BK;
RA   Daniel R., Gottschalk G.;
RL   Submitted (MAY-1994) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Transfers a methylene group from S-adenosyl-L-methionine to
CC       the cis double bond of an unsaturated fatty acid chain resulting in the
CC       replacement of the double bond with a methylene bridge. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1-acyl-2-(9Z)-enoyl-sn-glycero-3-phospholipid + S-adenosyl-
CC         L-methionine = a 1-acyl-2-(9-cyclopronane)-acyl-sn-glycero-3-
CC         phospholipid + S-adenosyl-L-homocysteine + H(+);
CC         Xref=Rhea:RHEA:11988, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856,
CC         ChEBI:CHEBI:59789, ChEBI:CHEBI:76593, ChEBI:CHEBI:76594; EC=2.1.1.79;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CFA/CMAS family. {ECO:0000305}.
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DR   EMBL; U09771; AAB48842.1; -; Genomic_DNA.
DR   AlphaFoldDB; P45509; -.
DR   SMR; P45509; -.
DR   STRING; 1333848.CFNIH1_02655; -.
DR   PaxDb; 1333848-CFNIH1_02655; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008825; F:cyclopropane-fatty-acyl-phospholipid synthase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR050723; CFA/CMAS.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR43667; CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASE; 1.
DR   PANTHER; PTHR43667:SF1; CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASE; 1.
DR   Pfam; PF02353; CMAS; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Lipid biosynthesis; Lipid metabolism; Methyltransferase;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           <1..89
FT                   /note="Cyclopropane-fatty-acyl-phospholipid synthase"
FT                   /id="PRO_0000089570"
FT   ACT_SITE        61
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   89 AA;  10609 MW;  7D0DDB9F377F72EA CRC64;
     ISHIAEASES RFVMEDWHNF GSDYDKTLMA WHERFNQAWP ELSSRYSATF RRMFNYYLCA
     CAGAFRARDI ELWQVLFSRG VEGGIRVYR
//