ID   THLA_CLODI              Reviewed;         107 AA.
AC   P45362;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 2.
DT   02-SEP-2026, entry version 98.
DE   RecName: Full=Acetyl-CoA acetyltransferase {ECO:0000250|UniProtKB:P45359};
DE            EC=2.3.1.9 {ECO:0000250|UniProtKB:P45359};
DE   AltName: Full=Acetoacetyl-CoA thiolase {ECO:0000250|UniProtKB:P45359};
DE   Flags: Fragment;
GN   Name=thi;
OS   Clostridioides difficile (Peptoclostridium difficile).
OC   Bacteria; Bacillati; Bacillota; Clostridia; Peptostreptococcales;
OC   Peptostreptococcaceae; Clostridioides.
OX   NCBI_TaxID=1496;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=E;
RX   PubMed=8001771; DOI=10.1111/j.1574-6968.1994.tb07262.x;
RA   Mullany P., Clayton C.L., Pallen M.J., Slone R., Al-Saleh A.,
RA   Tabaqchali S.;
RT   "Genes encoding homologues of three consecutive enzymes in the
RT   butyrate/butanol-producing pathway of Clostridium acetobutylicum are
RT   clustered on the Clostridium difficile chromosome.";
RL   FEMS Microbiol. Lett. 124:61-67(1994).
CC   -!- FUNCTION: Catalyzes the condensation of two molecules of acetyl-CoA to
CC       produce acetoacetyl-CoA. {ECO:0000250|UniProtKB:P45359}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 acetyl-CoA = acetoacetyl-CoA + CoA; Xref=Rhea:RHEA:21036,
CC         ChEBI:CHEBI:57286, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.9;
CC         Evidence={ECO:0000250|UniProtKB:P45359};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:P45359}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P45359}.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA56273.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X79899; CAA56273.1; ALT_INIT; Genomic_DNA.
DR   PIR; I40680; I40680.
DR   AlphaFoldDB; P45362; -.
DR   SMR; P45362; -.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0003985; F:acetyl-CoA C-acetyltransferase activity; ISS:UniProtKB.
DR   Gene3D; 3.40.47.10; -; 1.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR020616; Thiolase_N.
DR   PANTHER; PTHR18919:SF107; ACETYL-COA ACETYLTRANSFERASE, CYTOSOLIC; 1.
DR   PANTHER; PTHR18919; ACETYL-COA C-ACYLTRANSFERASE; 1.
DR   Pfam; PF00108; Thiolase_N; 1.
DR   SUPFAM; SSF53901; Thiolase-like; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Cytoplasm; Transferase.
FT   CHAIN           1..>107
FT                   /note="Acetyl-CoA acetyltransferase"
FT                   /id="PRO_0000206404"
FT   ACT_SITE        88
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000250|UniProtKB:P45359"
FT   NON_TER         107
SQ   SEQUENCE   107 AA;  11101 MW;  CE702BBFB1AF0713 CRC64;
     MREVVIASAA RTAVGSFGGA FKSVSAVELG VTAAKEAIKR ANITPDMIDE SLLGGVLTAG
     LGQNIARQIA LGAGIPVEKP AMTINIVCGS GLRSWRALRT RTGPRRR
//