ID AGP3_YEAST Reviewed; 558 AA. AC P43548; D6VTH5; DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1995, sequence version 1. DT 10-JUN-2026, entry version 180. DE RecName: Full=General amino acid permease AGP3; GN Name=AGP3; OrderedLocusNames=YFL055W; OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. OX NCBI_TaxID=559292; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 204508 / S288c; RX PubMed=7670463; DOI=10.1038/ng0795-261; RA Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., RA Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., RA Eki T.; RT "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces RT cerevisiae."; RL Nat. Genet. 10:261-268(1995). RN [2] RP GENOME REANNOTATION. RC STRAIN=ATCC 204508 / S288c; RX PubMed=24374639; DOI=10.1534/g3.113.008995; RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.; RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now."; RL G3 (Bethesda) 4:389-398(2014). RN [3] RP CHARACTERIZATION. RA Garrett J.M., Schreve J.L.; RL Unpublished observations (JUL-1997). RN [4] RP TOPOLOGY [LARGE SCALE ANALYSIS]. RC STRAIN=ATCC 208353 / W303-1A; RX PubMed=16847258; DOI=10.1073/pnas.0604075103; RA Kim H., Melen K., Oesterberg M., von Heijne G.; RT "A global topology map of the Saccharomyces cerevisiae membrane proteome."; RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006). CC -!- FUNCTION: General amino acid permease with broad substrate specificity. CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC) CC superfamily. YAT (TC 2.A.3.10) family. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D50617; BAA09186.1; -; Genomic_DNA. DR EMBL; BK006940; DAA12385.1; -; Genomic_DNA. DR PIR; S56200; S56200. DR RefSeq; NP_116600.1; NM_001179912.1. DR AlphaFoldDB; P43548; -. DR SMR; P43548; -. DR BioGRID; 31092; 53. DR DIP; DIP-4160N; -. DR FunCoup; P43548; 185. DR IntAct; P43548; 3. DR MINT; P43548; -. DR STRING; 4932.YFL055W; -. DR TCDB; 2.A.3.10.14; the amino acid-polyamine-organocation (apc) family. DR iPTMnet; P43548; -. DR PaxDb; 4932-YFL055W; -. DR EnsemblFungi; YFL055W_mRNA; YFL055W; YFL055W. DR GeneID; 850489; -. DR KEGG; sce:YFL055W; -. DR AGR; SGD:S000001839; -. DR SGD; S000001839; AGP3. DR VEuPathDB; FungiDB:YFL055W; -. DR eggNOG; KOG1286; Eukaryota. DR HOGENOM; CLU_007946_12_2_1; -. DR InParanoid; P43548; -. DR OMA; WRILFVY; -. DR OrthoDB; 3900342at2759; -. DR BioCyc; YEAST:G3O-30411-MONOMER; -. DR SABIO-RK; P43548; -. DR BioGRID-ORCS; 850489; 5 hits in 10 CRISPR screens. DR PRO; PR:P43548; -. DR Proteomes; UP000002311; Chromosome VI. DR RNAct; P43548; protein. DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD. DR GO; GO:0016020; C:membrane; IBA:GO_Central. DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IDA:SGD. DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central. DR GO; GO:0006865; P:amino acid transport; IDA:SGD. DR GO; GO:0055085; P:transmembrane transport; IDA:SGD. DR FunFam; 1.20.1740.10:FF:000001; Amino acid permease; 1. DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1. DR InterPro; IPR004841; AA-permease/SLC12A_dom. DR InterPro; IPR004840; Amino_acid_permease_CS. DR InterPro; IPR050524; APC_YAT. DR PANTHER; PTHR43341; AMINO ACID PERMEASE; 1. DR PANTHER; PTHR43341:SF26; GENERAL AMINO ACID PERMEASE AGP3; 1. DR Pfam; PF00324; AA_permease; 1. DR PIRSF; PIRSF006060; AA_transporter; 1. DR PROSITE; PS00218; AMINO_ACID_PERMEASE_1; 1. PE 1: Evidence at protein level; KW Amino-acid transport; Membrane; Reference proteome; Transmembrane; KW Transmembrane helix; Transport. FT CHAIN 1..558 FT /note="General amino acid permease AGP3" FT /id="PRO_0000054144" FT TOPO_DOM 1..57 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 58..78 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 79..81 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 82..102 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 103..126 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 127..147 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 148..168 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 169..189 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 190..193 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 194..214 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 215..241 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 242..262 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 263..280 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 281..301 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 302..324 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 325..345 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 346..375 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 376..396 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 397..402 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 403..423 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 424..446 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" FT TRANSMEM 447..467 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 468..480 FT /note="Extracellular" FT /evidence="ECO:0000255" FT TRANSMEM 481..501 FT /note="Helical" FT /evidence="ECO:0000255" FT TOPO_DOM 502..558 FT /note="Cytoplasmic" FT /evidence="ECO:0000255" SQ SEQUENCE 558 AA; 61052 MW; D7AE03BE36ADA1A1 CRC64; MAVLNLKRET VDIEETAKKD IKPYFASNVE AVDIDEDPDV SRYDPQTGVK RALKNRHISL LALGGVIGPG CLVGAGNALN KGGPLALLLG FSIIGIIAFS VMESIGEMIT LYPSGGGFTT LARRFHSDAL PAVCGYAYVV VFFAVLANEY NTLSSILQFW GPQVPLYGYI LIFWFAFEIF QLVGVGLFGE TEYWLAWLKI VGLVAYYIFS IVYISGDIRN RPAFGFHYWN SPGALSHGFK GIAIVFVFCS TFYSGTESVA LAATESKNPG KAVPLAVRQT LWRILVVYIG IAVFYGATVP FDDPNLSAST KVLKSPIAIA ISRAGWAGGA HLVNAFILIT CISAINGSLY IGSRTLTHLA HEGLAPKILA WTDRRGVPIP AITVFNALGL ISLMNVSVGA ANAYSYIVNL SGVGVFIVWG VISYTHLRIR KAWVAQGRSI EELPYEALFY PWTPVLSLAA NIFLALIQGW SYFVPFDAGN FVDAYILLPV GILLYIGICV FKSNHFRTVD LRSINLDEGR RKDMEADLSD QESSLASSET MKDYKSATFF RYLSNIFT //