ID   MAGA9_HUMAN             Reviewed;         315 AA.
AC   P43362; A8K8A7; Q7Z5K4; Q92910;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   02-SEP-2026, entry version 181.
DE   RecName: Full=Melanoma-associated antigen 9;
DE   AltName: Full=Cancer/testis antigen 1.9;
DE            Short=CT1.9;
DE   AltName: Full=MAGE-9 antigen;
GN   Name=MAGEA9; Synonyms=MAGE9, MAGEA9A;
GN   and
GN   Name=MAGEA9B;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=7927540; DOI=10.1007/bf01246677;
RA   De Plaen E., Arden K., Traversari C., Gaforio J.J., Szikora J.-P.,
RA   De Smet C., Brasseur F., van der Bruggen P., Lethe B.G., Lurquin C.,
RA   Brasseur R., Chomez P., de Backer O., Cavenee W., Boon T.;
RT   "Structure, chromosomal localization, and expression of 12 genes of the
RT   MAGE family.";
RL   Immunogenetics 40:360-369(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9147653; DOI=10.1093/hmg/6.3.479;
RA   Timms K.M., Bondeson M.-L., Ansari-Lari M.A., Lagerstedt K., Muzny D.M.,
RA   Dugan-Rocha S.P., Nelson D.L., Pettersson U., Gibbs R.A.;
RT   "Molecular and phenotypic variation in patients with severe Hunter
RT   syndrome.";
RL   Hum. Mol. Genet. 6:479-486(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Liver;
RA   Zhu J., Feng Z., Guan X.;
RT   "MAGE-9 antigen (MAGE9) gene expressed in human hepatocellular carcinoma
RT   patients.";
RL   Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Esophagus, and Testis;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA   Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA   Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA   Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA   Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA   Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA   Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA   Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA   Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA   Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA   Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA   Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA   Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA   Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA   Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA   Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA   Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA   Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA   Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA   Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA   Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA   Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA   Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA   Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA   Hunkapiller M.W., Myers E.W., Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Lung;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [7]
RP   VARIANT HIS-31.
RX   PubMed=20598277; DOI=10.1016/j.ajhg.2010.06.008;
RA   Sun Y., Almomani R., Aten E., Celli J., van der Heijden J., Venselaar H.,
RA   Robertson S.P., Baroncini A., Franco B., Basel-Vanagaite L., Horii E.,
RA   Drut R., Ariyurek Y., den Dunnen J.T., Breuning M.H.;
RT   "Terminal osseous dysplasia is caused by a single recurrent mutation in the
RT   FLNA gene.";
RL   Am. J. Hum. Genet. 87:146-153(2010).
CC   -!- FUNCTION: Not known, though may play a role in embryonal development
CC       and tumor transformation or aspects of tumor progression.
CC   -!- INTERACTION:
CC       P43362; Q9UKG1: APPL1; NbExp=5; IntAct=EBI-10209139, EBI-741243;
CC   -!- TISSUE SPECIFICITY: Expressed in many tumors of several types, such as
CC       melanoma, head and neck squamous cell carcinoma, lung carcinoma and
CC       breast carcinoma, but not in normal tissues except for testes and
CC       placenta.
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DR   EMBL; U10694; AAA68877.1; -; Genomic_DNA.
DR   EMBL; U66083; AAB67888.1; -; Genomic_DNA.
DR   EMBL; AY310325; AAP82171.1; -; mRNA.
DR   EMBL; AK292272; BAF84961.1; -; mRNA.
DR   EMBL; AK313321; BAG36126.1; -; mRNA.
DR   EMBL; CH471169; EAW99372.1; -; Genomic_DNA.
DR   EMBL; BC002351; AAH02351.1; -; mRNA.
DR   CCDS; CCDS14691.1; -.
DR   CCDS; CCDS35423.1; -.
DR   PIR; I38668; I38668.
DR   RefSeq; NP_001074259.1; NM_001080790.1.
DR   RefSeq; NP_005356.1; NM_005365.5.
DR   RefSeq; XP_005262391.1; XM_005262334.4.
DR   RefSeq; XP_005262392.1; XM_005262335.4.
DR   RefSeq; XP_005278249.1; XM_005278192.4.
DR   RefSeq; XP_005278250.1; XM_005278193.4.
DR   RefSeq; XP_024308149.1; XM_024452381.2.
DR   RefSeq; XP_024308150.1; XM_024452382.2.
DR   RefSeq; XP_054183037.1; XM_054327062.1.
DR   RefSeq; XP_054183038.1; XM_054327063.1.
DR   AlphaFoldDB; P43362; -.
DR   SMR; P43362; -.
DR   BioGRID; 110282; 220.
DR   BioGRID; 608698; 7.
DR   FunCoup; P43362; 86.
DR   IntAct; P43362; 212.
DR   STRING; 9606.ENSP00000243314; -.
DR   iPTMnet; P43362; -.
DR   PhosphoSitePlus; P43362; -.
DR   BioMuta; MAGEA9B; -.
DR   DMDM; 1170862; -.
DR   jPOST; P43362; -.
DR   MassIVE; P43362; -.
DR   PaxDb; 9606-ENSP00000243314; -.
DR   PeptideAtlas; P43362; -.
DR   ProteomicsDB; 55624; -.
DR   Pumba; P43362; -.
DR   Antibodypedia; 16920; 305 antibodies from 31 providers.
DR   Antibodypedia; 72928; 2 antibodies from 1 providers.
DR   DNASU; 4108; -.
DR   Ensembl; ENST00000243314.5; ENSP00000243314.5; ENSG00000123584.7.
DR   Ensembl; ENST00000595065.6; ENSP00000471017.1; ENSG00000267978.7.
DR   Ensembl; ENST00000950133.1; ENSP00000620192.1; ENSG00000267978.7.
DR   GeneID; 4108; -.
DR   GeneID; 728269; -.
DR   KEGG; hsa:4108; -.
DR   KEGG; hsa:728269; -.
DR   MANE-Select; ENST00000243314.5; ENSP00000243314.5; NM_005365.5; NP_005356.1.
DR   MANE-Select; ENST00000595065.6; ENSP00000471017.1; NM_001080790.1; NP_001074259.1.
DR   UCSC; uc004fdk.4; human.
DR   AGR; HGNC:31909; -.
DR   AGR; HGNC:6807; -.
DR   ClinPGx; PA145148473; -.
DR   CTD; 4108; -.
DR   CTD; 728269; -.
DR   DisGeNET; 4108; -.
DR   DisGeNET; 728269; -.
DR   GeneCards; MAGEA9; -.
DR   GeneCards; MAGEA9B; -.
DR   HGNC; HGNC:6807; MAGEA9.
DR   HGNC; HGNC:31909; MAGEA9B.
DR   HPA; ENSG00000123584; Tissue enriched (testis).
DR   HPA; ENSG00000267978; Tissue enriched (testis).
DR   MIM; 300342; gene.
DR   MIM; 300764; gene.
DR   OpenTargets; ENSG00000123584; -.
DR   OpenTargets; ENSG00000267978; -.
DR   VEuPathDB; HostDB:ENSG00000123584; -.
DR   VEuPathDB; HostDB:ENSG00000267978; -.
DR   eggNOG; KOG4562; Eukaryota.
DR   GeneTree; ENSGT00940000165994; -.
DR   HOGENOM; CLU_039582_1_2_1; -.
DR   InParanoid; P43362; -.
DR   OMA; TCKSAFV; -.
DR   OrthoDB; 9538249at2759; -.
DR   PAN-GO; P43362; 3 GO annotations based on evolutionary models.
DR   PhylomeDB; P43362; -.
DR   PathwayCommons; P43362; -.
DR   SignaLink; P43362; -.
DR   Agora; ENSG00000123584; -.
DR   BioGRID-ORCS; 4108; 12 hits in 628 CRISPR screens.
DR   BioGRID-ORCS; 728269; 20 hits in 635 CRISPR screens.
DR   ChiTaRS; MAGEA9B; human.
DR   GeneWiki; MAGEA9; -.
DR   Pharos; P43362; Tbio.
DR   PRO; PR:P43362; -.
DR   Proteomes; UP000005640; Chromosome X.
DR   RNAct; P43362; protein.
DR   Bgee; ENSG00000123584; Expressed in male germ line stem cell (sensu Vertebrata) in testis and 17 other cell types or tissues.
DR   ExpressionAtlas; P43362; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0042826; F:histone deacetylase binding; IBA:GO_Central.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   FunFam; 1.10.10.1200:FF:000002; MAGE family member A11; 1.
DR   FunFam; 1.10.10.1210:FF:000001; melanoma-associated antigen D1; 1.
DR   Gene3D; 1.10.10.1200; MAGE homology domain, winged helix WH1 motif; 1.
DR   Gene3D; 1.10.10.1210; MAGE homology domain, winged helix WH2 motif; 1.
DR   InterPro; IPR037445; MAGE.
DR   InterPro; IPR021072; MAGE_N.
DR   InterPro; IPR041898; MAGE_WH1.
DR   InterPro; IPR041899; MAGE_WH2.
DR   InterPro; IPR002190; MHD_dom.
DR   PANTHER; PTHR11736:SF62; MELANOMA-ASSOCIATED ANTIGEN 9; 1.
DR   PANTHER; PTHR11736; MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN; 1.
DR   Pfam; PF01454; MAGE; 1.
DR   Pfam; PF12440; MAGE_N; 1.
DR   SMART; SM01373; MAGE; 1.
DR   SMART; SM01392; MAGE_N; 1.
DR   PROSITE; PS50838; MAGE; 1.
PE   1: Evidence at protein level;
KW   Proteomics identification; Reference proteome; Tumor antigen.
FT   CHAIN           1..315
FT                   /note="Melanoma-associated antigen 9"
FT                   /id="PRO_0000156708"
FT   DOMAIN          108..307
FT                   /note="MAGE"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00127"
FT   REGION          1..67
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..13
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        50..67
FT                   /note="Low complexity"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   VARIANT         31
FT                   /note="P -> H (in dbSNP:rs202146513)"
FT                   /evidence="ECO:0000269|PubMed:20598277"
FT                   /id="VAR_064160"
FT   CONFLICT        89
FT                   /note="E -> G (in Ref. 3; AAP82171)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        309
FT                   /note="G -> R (in Ref. 3; AAP82171)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        312
FT                   /note="Q -> E (in Ref. 3; AAP82171)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   315 AA;  35088 MW;  7FD2ED10D680D928 CRC64;
     MSLEQRSPHC KPDEDLEAQG EDLGLMGAQE PTGEEEETTS SSDSKEEEVS AAGSSSPPQS
     PQGGASSSIS VYYTLWSQFD EGSSSQEEEE PSSSVDPAQL EFMFQEALKL KVAELVHFLL
     HKYRVKEPVT KAEMLESVIK NYKRYFPVIF GKASEFMQVI FGTDVKEVDP AGHSYILVTA
     LGLSCDSMLG DGHSMPKAAL LIIVLGVILT KDNCAPEEVI WEALSVMGVY VGKEHMFYGE
     PRKLLTQDWV QENYLEYRQV PGSDPAHYEF LWGSKAHAET SYEKVINYLV MLNAREPICY
     PSLYEEVLGE EQEGV
//