ID   PHS_PSEAE               Reviewed;         118 AA.
AC   P43335;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   02-SEP-2026, entry version 128.
DE   RecName: Full=Pterin-4-alpha-carbinolamine dehydratase;
DE            Short=PHS;
DE            EC=4.2.1.96;
DE   AltName: Full=4-alpha-hydroxy-tetrahydropterin dehydratase;
DE   AltName: Full=Pterin carbinolamine dehydratase;
DE            Short=PCD;
GN   Name=phhB; OrderedLocusNames=PA0871;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
OC   Pseudomonadales; Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 /
RC   1C / PRS 101 / PAO1;
RX   PubMed=8108417; DOI=10.1073/pnas.91.4.1366;
RA   Zhao G., Xia T., Song J., Roy R.A.;
RT   "Pseudomonas aeruginosa possesses homologues of mammalian phenylalanine
RT   hydroxylase and 4 alpha-carbinolamine dehydratase/DCoH as part of a three-
RT   component gene cluster.";
RL   Proc. Natl. Acad. Sci. U.S.A. 91:1366-1370(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 /
RC   1C / PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Involved in tetrahydrobiopterin biosynthesis. Seems to both
CC       prevent the formation of 7-pterins and accelerate the formation of
CC       quinonoid-BH2. May also have a positive regulatory role in the
CC       expression of phhA.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin =
CC         (6R)-L-erythro-6,7-dihydrobiopterin + H2O; Xref=Rhea:RHEA:11920,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15642, ChEBI:CHEBI:43120; EC=4.2.1.96;
CC   -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the pterin-4-alpha-carbinolamine dehydratase
CC       family. {ECO:0000305}.
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DR   EMBL; M88627; AAA25937.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG04260.1; -; Genomic_DNA.
DR   PIR; E83535; E83535.
DR   RefSeq; NP_249562.1; NC_002516.2.
DR   RefSeq; WP_003085898.1; NC_002516.2.
DR   AlphaFoldDB; P43335; -.
DR   SMR; P43335; -.
DR   STRING; 208964.PA0871; -.
DR   PaxDb; 208964-PA0871; -.
DR   GeneID; 880827; -.
DR   KEGG; pae:PA0871; -.
DR   PATRIC; fig|208964.12.peg.905; -.
DR   PseudoCAP; PA0871; -.
DR   HOGENOM; CLU_081974_2_2_6; -.
DR   InParanoid; P43335; -.
DR   OrthoDB; 5294615at2; -.
DR   PhylomeDB; P43335; -.
DR   BioCyc; PAER208964:G1FZ6-886-MONOMER; -.
DR   Proteomes; UP000002438; Chromosome.
DR   AbasyAtlas; P43335; -.
DR   GO; GO:0008124; F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006729; P:tetrahydrobiopterin biosynthetic process; IEA:InterPro.
DR   CDD; cd00913; PCD_DCoH_subfamily_a; 1.
DR   Gene3D; 3.30.1360.20; Transcriptional coactivator/pterin dehydratase; 1.
DR   HAMAP; MF_00434; Pterin_4_alpha; 1.
DR   InterPro; IPR036428; PCD_sf.
DR   InterPro; IPR050376; Pterin-4-alpha-carb_dehyd.
DR   InterPro; IPR001533; Pterin_deHydtase.
DR   NCBIfam; NF002016; PRK00823.1-1; 1.
DR   PANTHER; PTHR42805; PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE-RELATED; 1.
DR   PANTHER; PTHR42805:SF1; PTERIN-4-ALPHA-CARBINOLAMINE DEHYDRATASE-RELATED; 1.
DR   Pfam; PF01329; Pterin_4a; 1.
DR   SUPFAM; SSF55248; PCD-like; 1.
PE   3: Inferred from homology;
KW   Lyase; Reference proteome; Tetrahydrobiopterin biosynthesis.
FT   CHAIN           1..118
FT                   /note="Pterin-4-alpha-carbinolamine dehydratase"
FT                   /id="PRO_0000063065"
SQ   SEQUENCE   118 AA;  13333 MW;  02502CBC1F9ADF94 CRC64;
     MTALTQAHCE ACRADAPHVS DEELPVLLRQ IPDWNIEVRD GIMQLEKVYL FKNFKHALAF
     TNAVGEISEA EGHHPGLLTE WGKVTVTWWS HSIKGLHRND FIMAARTDEV AKTAEGRK
//