ID   SRK3_SPOLA              Reviewed;         334 AA.
AC   P42689;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   02-SEP-2026, entry version 114.
DE   RecName: Full=Tyrosine-protein kinase SRK3;
DE            EC=2.7.10.2;
DE   Flags: Fragment;
GN   Name=SRK3;
OS   Spongilla lacustris (Freshwater sponge) (Spongia lacustris).
OC   Eukaryota; Metazoa; Porifera; Demospongiae; Heteroscleromorpha;
OC   Spongillida; Spongillidae; Spongilla.
OX   NCBI_TaxID=6055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=1378585;
RA   Ottilie S., Raulf F., Barnekow A., Hannig G., Schartl M.;
RT   "Multiple src-related kinase genes, srk1-4, in the fresh water sponge
RT   Spongilla lacustris.";
RL   Oncogene 7:1625-1630(1992).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-tyrosyl-[protein] + ATP = O-phospho-L-tyrosyl-[protein] +
CC         ADP + H(+); Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC         COMP:20101, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC         ChEBI:CHEBI:61978, ChEBI:CHEBI:456216; EC=2.7.10.2;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10028};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein
CC       kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
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DR   EMBL; X61603; CAA43800.1; -; mRNA.
DR   PIR; S24552; S24552.
DR   AlphaFoldDB; P42689; -.
DR   SMR; P42689; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:UniProtKB-EC.
DR   CDD; cd05034; PTKc_Src_like; 1.
DR   FunFam; 1.10.510.10:FF:000318; Tyrosine-protein kinase; 1.
DR   FunFam; 3.30.200.20:FF:000037; Tyrosine-protein kinase; 1.
DR   Gene3D; 3.30.200.20; Phosphorylase Kinase, domain 1; 1.
DR   Gene3D; 3.30.505.10; SH2 domain; 1.
DR   Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR050198; Non-receptor_tyrosine_kinases.
DR   InterPro; IPR000719; Prot_kinase_dom.
DR   InterPro; IPR017441; Protein_kinase_ATP_BS.
DR   InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR   InterPro; IPR000980; SH2.
DR   InterPro; IPR036860; SH2_dom_sf.
DR   InterPro; IPR008266; Tyr_kinase_AS.
DR   InterPro; IPR020635; Tyr_kinase_cat_dom.
DR   PANTHER; PTHR24418; TYROSINE-PROTEIN KINASE; 1.
DR   Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR   PIRSF; PIRSF000615; TyrPK_CSF1-R; 1.
DR   PRINTS; PR00109; TYRKINASE.
DR   SMART; SM00219; TyrKc; 1.
DR   SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR   SUPFAM; SSF55550; SH2 domain; 1.
DR   PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR   PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR   PROSITE; PS00109; PROTEIN_KINASE_TYR; 1.
DR   PROSITE; PS50001; SH2; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Kinase; Nucleotide-binding; Phosphoprotein;
KW   SH2 domain; Transferase; Tyrosine-protein kinase.
FT   CHAIN           <1..334
FT                   /note="Tyrosine-protein kinase SRK3"
FT                   /id="PRO_0000088159"
FT   DOMAIN          <1..42
FT                   /note="SH2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT   DOMAIN          66..321
FT                   /note="Protein kinase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   ACT_SITE        186
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT                   ECO:0000255|PROSITE-ProRule:PRU10028"
FT   BINDING         72..80
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   BINDING         94
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT   NON_TER         1
SQ   SEQUENCE   334 AA;  37880 MW;  2AFEB9C409817AA2 CRC64;
     IRTLDDGGFY MANRISFPTL QNLVSHYMMD ADGLAQRLSR PCSRANIPIT SGLSYKDEWE
     IDRTTIQLQR KLGQGNFGEV WAGVWNGTTA VAVKTLKPDT MEVKDFVQEA QVMKKIHHPN
     LLQLYAVCTI GEPIYIVTEL MKYGSMLEYL KHGEGKNITL HQMVDMSAQI ASGMTYLEAH
     SYIHRDLAAR NILVGEGNVC KVADFGLARV IKEDIYNPRE GTKFPIKWTA PEAALYNRFT
     IKSDVWSFGV LISEIVTHGR MPYPGMTNRQ VLEAVDRGYR MPCPEGCPDP LYKIMLSCWK
     HEPDDRPTFE SLKNLLEDYY VSAAEGSYRE PAPR
//