ID   MSH4_YEAST              Reviewed;         878 AA.
AC   P40965; D6VTM6;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2010, sequence version 2.
DT   02-SEP-2026, entry version 186.
DE   RecName: Full=MutS protein homolog 4;
GN   Name=MSH4; OrderedLocusNames=YFL003C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8001134; DOI=10.1016/0092-8674(94)90037-x;
RA   Ross-Macdonald P., Roeder G.S.;
RT   "Mutation of a meiosis-specific MutS homolog decreases crossing over but
RT   not mismatch correction.";
RL   Cell 79:1069-1080(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7670463; DOI=10.1038/ng0795-261;
RA   Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S.,
RA   Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H.,
RA   Eki T.;
RT   "Analysis of the nucleotide sequence of chromosome VI from Saccharomyces
RT   cerevisiae.";
RL   Nat. Genet. 10:261-268(1995).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204511 / S288c / AB972;
RX   PubMed=8789262;
RX   DOI=10.1002/(sici)1097-0061(199601)12:1%3c77::aid-yea887%3e3.0.co;2-5;
RA   Naitou M., Ozawa M., Sasanuma S., Kobayashi M., Hagiwara H., Shibata T.,
RA   Hanaoka F., Watanabe K., Ono A., Yamazaki M., Tashiro H., Eki T.,
RA   Murakami Y.;
RT   "Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome
RT   VI.";
RL   Yeast 12:77-84(1996).
RN   [5]
RP   INTERACTION WITH MSH5.
RX   PubMed=9374523; DOI=10.1074/jbc.272.48.30345;
RA   Pochart P., Woltering D., Hollingsworth N.M.;
RT   "Conserved properties between functionally distinct MutS homologs in
RT   yeast.";
RL   J. Biol. Chem. 272:30345-30349(1997).
CC   -!- FUNCTION: Involved in meiotic recombination. Facilitate crossovers
CC       between homologs during meiosis.
CC   -!- SUBUNIT: Heterooligomer of MSH4 and MSH5. {ECO:0000269|PubMed:9374523}.
CC   -!- INTERACTION:
CC       P40965; Q12175: MSH5; NbExp=2; IntAct=EBI-11371, EBI-11377;
CC   -!- MISCELLANEOUS: Two distinct classes of crossovers have been
CC       demonstrated in budding yeast. Class I is MSH4/MSH5 dependent and
CC       exhibits crossover interference. Class II is MUS81/MMS4 dependent and
CC       exhibits no interference.
CC   -!- SIMILARITY: Belongs to the DNA mismatch repair MutS family.
CC       {ECO:0000305}.
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DR   EMBL; U13999; AAA65234.1; -; Genomic_DNA.
DR   EMBL; D50617; BAA09235.1; -; Genomic_DNA.
DR   EMBL; BK006940; DAA12436.1; -; Genomic_DNA.
DR   PIR; A55201; A55201.
DR   RefSeq; NP_116652.1; NM_001179963.1.
DR   AlphaFoldDB; P40965; -.
DR   SMR; P40965; -.
DR   BioGRID; 31143; 293.
DR   ComplexPortal; CPX-1704; MutSgamma meiotic recombination complex.
DR   DIP; DIP-2426N; -.
DR   FunCoup; P40965; 475.
DR   IntAct; P40965; 5.
DR   MINT; P40965; -.
DR   STRING; 4932.YFL003C; -.
DR   iPTMnet; P40965; -.
DR   PaxDb; 4932-YFL003C; -.
DR   PeptideAtlas; P40965; -.
DR   EnsemblFungi; YFL003C_mRNA; YFL003C; YFL003C.
DR   GeneID; 850545; -.
DR   KEGG; sce:YFL003C; -.
DR   AGR; SGD:S000001891; -.
DR   SGD; S000001891; MSH4.
DR   VEuPathDB; FungiDB:YFL003C; -.
DR   eggNOG; KOG0220; Eukaryota.
DR   HOGENOM; CLU_002472_7_3_1; -.
DR   InParanoid; P40965; -.
DR   OMA; KMTMLYK; -.
DR   OrthoDB; 276261at2759; -.
DR   BioCyc; YEAST:G3O-30451-MONOMER; -.
DR   BioGRID-ORCS; 850545; 1 hit in 10 CRISPR screens.
DR   PRO; PR:P40965; -.
DR   Proteomes; UP000002311; Chromosome VI.
DR   RNAct; P40965; protein.
DR   GO; GO:1990391; C:DNA repair complex; IPI:ComplexPortal.
DR   GO; GO:0062128; C:MutSgamma complex; IPI:SGD.
DR   GO; GO:0000228; C:nuclear chromosome; IDA:SGD.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0140664; F:ATP-dependent DNA damage sensor activity; IEA:InterPro.
DR   GO; GO:0030983; F:mismatched DNA binding; IEA:InterPro.
DR   GO; GO:0007131; P:reciprocal meiotic recombination; IMP:SGD.
DR   CDD; cd03282; ABC_MSH4_euk; 1.
DR   FunFam; 3.30.420.110:FF:000025; Msh4p; 1.
DR   Gene3D; 1.10.1420.10; -; 2.
DR   Gene3D; 3.30.420.110; MutS, connector domain; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   InterPro; IPR011184; DNA_mismatch_repair_Msh2.
DR   InterPro; IPR000432; DNA_mismatch_repair_MutS_C.
DR   InterPro; IPR007861; DNA_mismatch_repair_MutS_clamp.
DR   InterPro; IPR007696; DNA_mismatch_repair_MutS_core.
DR   InterPro; IPR036187; DNA_mismatch_repair_MutS_sf.
DR   InterPro; IPR007860; DNA_mmatch_repair_MutS_con_dom.
DR   InterPro; IPR045076; MutS.
DR   InterPro; IPR036678; MutS_con_dom_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR11361; DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER; 1.
DR   PANTHER; PTHR11361:SF21; MUTS PROTEIN HOMOLOG 4; 1.
DR   Pfam; PF05188; MutS_II; 1.
DR   Pfam; PF05192; MutS_III; 1.
DR   Pfam; PF05190; MutS_IV; 1.
DR   Pfam; PF00488; MutS_V; 1.
DR   PIRSF; PIRSF005813; MSH2; 1.
DR   SMART; SM00534; MUTSac; 1.
DR   SMART; SM00533; MUTSd; 1.
DR   SUPFAM; SSF53150; DNA repair protein MutS, domain II; 1.
DR   SUPFAM; SSF48334; DNA repair protein MutS, domain III; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00486; DNA_MISMATCH_REPAIR_2; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA-binding; Meiosis; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..878
FT                   /note="MutS protein homolog 4"
FT                   /id="PRO_0000115201"
FT   REGION          22..41
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         634..641
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        811..812
FT                   /note="IH -> MD (in Ref. 1; AAA65234)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   878 AA;  99225 MW;  BBA1CABF1A08954C CRC64;
     MSESNLSSFI STNYFNLRSA ANSSNSISKP STKKSIRNQK SPTNISSWAL KKKTLQIAET
     TWENNEKDST HSHYLMTGSM ASRTATSLSR YSTNASLLGP SIDCVLCCIY EVPRDISTRI
     GLCIINCNTG QMYLSDFMDS QIYIRVVHKL QIYQPTEILI PSSSLAPTVS KLATMIKFNV
     AETVKIEEGS RKCFNSQDGL AAITKYLMDD TKKDLKIEEI IDKTFALCAA SAAISYMEEI
     ISKSSRNLNA FRKLRIQFEG TENTMLIDSK TVRGLELVEN KLDKNGISLW KFLDTTSTKM
     GQRSLRNSIL QPLTDRGSIE MRLEALEELK ANDDLLQKLR LEMKSLPDLD KLFSRLLCIN
     HSAIKPDQRI NYVLLLKETL QSVKSLKDAL NDQLIQSRLI SETKKIFNND AIMEIEKLIN
     SCINEDCVWA SSAIQLLNQR SYAVKSDSNG LLDVSRQIYK EVKEEFFREV EDLTAKNKIN
     LDHNYDSARG FYLRIKRQEF TDDVATLPDV FISRTIKKNY IECTTLNIIK KNARLKEVME
     EILLLSEETV DELLDKIATH ISELFMIAEA VAILDLVCSF TYNLKENNYT IPIFTNNLLI
     RDSRHPLLEK VLKNFVPNTI SSTKHSSSLQ IITGCNMSGK SVYLKQVALI CIMAQMGSGI
     PALYGSFPVF KRLHARVCND SMELTSSNFG FEMKEMAYFL DDINTETLLI LDELGRGSSI
     ADGFCVSLAV TEHLLRTEAT VFLSTHFQDI PKIMSKKPAV SHLHMDAVLL NDNSVKMNYQ
     LTQKSVAIEN SGIRVVKKIF NPDIIAEAYN IHSLLKIAKA RTENEDSNGV VDQKTINQMK
     RIHNLVAILK ECAGNEKEPL TLGKLKEINS DFIENFEE
//