ID   VAB2_YEAST              Reviewed;         282 AA.
AC   P40003; D3DLP2;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   04-NOV-2026, entry version 147.
DE   RecName: Full=Biogenesis of lysosome-related organelles complex 1 subunit VAB2;
DE            Short=BLOC-1 subunit VAB2;
DE   AltName: Full=VAC8-binding protein 2;
GN   Name=VAB2; Synonyms=VAB31; OrderedLocusNames=YEL005C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169868; DOI=10.1038/387s078;
RA   Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA   Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA   Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA   Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA   Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA   Botstein D., Davis R.W.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL   Nature 387:78-81(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   INTERACTION WITH VAC8.
RX   PubMed=10888680; DOI=10.1091/mbc.11.7.2445;
RA   Pan X., Roberts P., Chen Y., Kvam E., Shulga N., Huang K., Lemmon S.,
RA   Goldfarb D.S.;
RT   "Nucleus-vacuole junctions in Saccharomyces cerevisiae are formed through
RT   the direct interaction of Vac8p with Nvj1p.";
RL   Mol. Biol. Cell 11:2445-2457(2000).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [5]
RP   IDENTIFICATION IN THE BLOC-1 COMPLEX, AND FUNCTION.
RX   PubMed=21159114; DOI=10.1111/j.1600-0854.2010.01151.x;
RA   Hayes M.J., Bryon K., Satkurunathan J., Levine T.P.;
RT   "Yeast homologues of three BLOC-1 subunits highlight KxDL proteins as
RT   conserved interactors of BLOC-1.";
RL   Traffic 12:260-268(2011).
CC   -!- FUNCTION: Component of the biogenesis of lysosome-related organelles
CC       complex-1 (BLOC-1) involved in endosomal cargo sorting.
CC       {ECO:0000269|PubMed:21159114}.
CC   -!- SUBUNIT: Component of the biogenesis of lysosome-related organelles
CC       complex-1 (BLOC-1) composed of at least BLI1, BLS1, CNL1, KXD1, SNN1
CC       and VAB2. Interacts with VAC8. {ECO:0000269|PubMed:10888680,
CC       ECO:0000269|PubMed:21159114}.
CC   -!- INTERACTION:
CC       P40003; P48232: SNN1; NbExp=4; IntAct=EBI-22275, EBI-28775;
CC       P40003; P39968: VAC8; NbExp=3; IntAct=EBI-22275, EBI-20212;
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle
CC       {ECO:0000269|PubMed:14562095}. Cytoplasm {ECO:0000269|PubMed:14562095}.
CC       Vacuole {ECO:0000269|PubMed:14562095}.
CC   -!- SIMILARITY: Belongs to the VAB2 family. {ECO:0000305}.
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DR   EMBL; U18530; AAB64482.1; -; Genomic_DNA.
DR   EMBL; BK006939; DAA07646.1; -; Genomic_DNA.
DR   PIR; S50454; S50454.
DR   RefSeq; NP_010911.3; NM_001178820.3.
DR   AlphaFoldDB; P40003; -.
DR   SMR; P40003; -.
DR   BioGRID; 36726; 64.
DR   ComplexPortal; CPX-1153; BLOC-1 complex.
DR   DIP; DIP-5277N; -.
DR   FunCoup; P40003; 54.
DR   IntAct; P40003; 12.
DR   STRING; 4932.YEL005C; -.
DR   iPTMnet; P40003; -.
DR   PaxDb; 4932-YEL005C; -.
DR   PeptideAtlas; P40003; -.
DR   EnsemblFungi; YEL005C_mRNA; YEL005C; YEL005C.
DR   GeneID; 856713; -.
DR   KEGG; sce:YEL005C; -.
DR   AGR; SGD:S000000731; -.
DR   SGD; S000000731; VAB2.
DR   VEuPathDB; FungiDB:YEL005C; -.
DR   eggNOG; ENOG502S95F; Eukaryota.
DR   HOGENOM; CLU_086126_0_0_1; -.
DR   InParanoid; P40003; -.
DR   OMA; AEEGCFY; -.
DR   OrthoDB; 4036527at2759; -.
DR   BioCyc; YEAST:G3O-30134-MONOMER; -.
DR   BioGRID-ORCS; 856713; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P40003; -.
DR   Proteomes; UP000002311; Chromosome V.
DR   RNAct; P40003; protein.
DR   GO; GO:0031083; C:BLOC-1 complex; IDA:SGD.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005768; C:endosome; IDA:ComplexPortal.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0007032; P:endosome organization; IMP:SGD.
DR   GO; GO:0032880; P:regulation of protein localization; IMP:SGD.
DR   InterPro; IPR063276; VAB2_CC.
DR   Pfam; PF29876; VAB2; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; Cytoplasmic vesicle; Reference proteome; Transport;
KW   Vacuole.
FT   CHAIN           1..282
FT                   /note="Biogenesis of lysosome-related organelles complex 1
FT                   subunit VAB2"
FT                   /id="PRO_0000202618"
FT   COILED          64..140
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   282 AA;  31365 MW;  8F7CA122F91E491A CRC64;
     MVADLTKGIL KWKSRIEFDA VGSSSYYEEL KGLPPLASHK KLTQAAIFNS TKYELLQVKK
     DILSIYEVVS RDIDEERNQM QQIELQLKKS LKKVEHSYKN VLKQRASTNC INGNDRLLAN
     AEKKIGSLNE ELACVNDIVS DIVNNLTALN ANLPKKAQLL KDDSINVAHY PLLFDFLHKS
     CPKSIIATSE ASIHENASPS PLLEHDELPA ESINSFYGEN ELQSDSLAPL QTHDDNISSC
     KKILPPKFNT TSGPSIETNF ENISADGLTY TKCSLKNSIS LT
//