ID   NIA_LOTTE               Reviewed;         160 AA.
AC   P39882;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   02-SEP-2026, entry version 89.
DE   RecName: Full=Nitrate reductase [NADH];
DE            Short=NR;
DE            EC=1.7.1.1;
DE   Flags: Fragment;
GN   Name=NIA;
OS   Lotus tetragonolobus (Winged pea) (Tetragonolobus purpureus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; robinioid clade; Loteae; Lotus.
OX   NCBI_TaxID=3868;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RA   Wu S., Lu Q., Kriz A.L., Harper J.E.;
RT   "Molecular cloning and analysis winged bean and soybean bispecific
RT   constitutive NR and their NiR.";
RL   Submitted (NOV-1993) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Nitrate reductase is a key enzyme involved in the first step
CC       of nitrate assimilation in plants, fungi and bacteria.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=nitrite + NAD(+) + H2O = nitrate + NADH + H(+);
CC         Xref=Rhea:RHEA:17913, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16301, ChEBI:CHEBI:17632, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.7.1.1;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
CC       Note=Binds 1 FAD. {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC       Note=Binds 1 heme group. The heme group is called cytochrome b-557.
CC       {ECO:0000250};
CC   -!- COFACTOR:
CC       Name=Mo-molybdopterin; Xref=ChEBI:CHEBI:71302; Evidence={ECO:0000250};
CC       Note=Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.
CC       {ECO:0000250};
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the nitrate reductase family. {ECO:0000305}.
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DR   EMBL; L16780; AAA80564.1; -; mRNA.
DR   AlphaFoldDB; P39882; -.
DR   SMR; P39882; -.
DR   GO; GO:0071949; F:FAD binding; ISS:UniProtKB.
DR   GO; GO:0009703; F:nitrate reductase (NADH) activity; IEA:UniProtKB-EC.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:TreeGrafter.
DR   GO; GO:0006809; P:nitric oxide biosynthetic process; IEA:TreeGrafter.
DR   CDD; cd06183; cyt_b5_reduct_like; 1.
DR   FunFam; 3.40.50.80:FF:000025; Nitrate reductase [NADH]; 1.
DR   Gene3D; 3.40.50.80; Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module; 1.
DR   InterPro; IPR001834; CBR-like.
DR   InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR   InterPro; IPR039261; FNR_nucleotide-bd.
DR   InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR   PANTHER; PTHR19370; NADH-CYTOCHROME B5 REDUCTASE; 1.
DR   PANTHER; PTHR19370:SF185; NADH-CYTOCHROME B5 REDUCTASE; 1.
DR   Pfam; PF00175; NAD_binding_1; 1.
DR   PRINTS; PR00406; CYTB5RDTASE.
DR   PRINTS; PR00371; FPNCR.
DR   SUPFAM; SSF52343; Ferredoxin reductase-like, C-terminal NADP-linked domain; 1.
PE   2: Evidence at transcript level;
KW   FAD; Flavoprotein; Heme; Iron; Metal-binding; Molybdenum; NAD;
KW   Nitrate assimilation; Oxidoreductase.
FT   CHAIN           <1..160
FT                   /note="Nitrate reductase [NADH]"
FT                   /id="PRO_0000166060"
FT   BINDING         37
FT                   /ligand="FAD"
FT                   /ligand_id="ChEBI:CHEBI:57692"
FT                   /evidence="ECO:0000250|UniProtKB:A0A286R227"
FT   NON_TER         1
SQ   SEQUENCE   160 AA;  17728 MW;  D6CFC1447393A932 CRC64;
     KGPLGHIEYT GKGNFIAHGE PKFARRLAML AGGTGITPIY QVIQAVLKDP DDRTEMFFAV
     YSNKTESDIL LREELDAWAK KHSDRFKVWY VVDKAGNDWA FSTGRVNESI MRVHLPGPSD
     ALALACGPPP INSAYGWQPS LENIGYKKDD VPVFMATTQS
//